Comparative Evaluation of Antimicrobial Activity of Selected Three Herbal Plants Extract with Digital Image Processing Technique

Abstract

Background and Objectives: The development in the technology have witnessed that there is a revival of interest in drug discovery from medicinal plants for treatment of the most destructive diseases. Our investigation characterizes the usage of digital image processing techniques in Matlab to process and analyze the antimicrobial effects of the selected herbal plants. Methods: The first stage of our investigation involves the extraction of components with methanol from the selected three herbal plants-Solanum xanthocarpum, Solanum nigrum and Helianthus annuus by using soxhlet apparatus. These plant extracts were assayed for antimicrobial activity against 4 different bacterial and fungal species using disk diffusion method. Results: Notable cell growth inhibitions were observed from the selected microbes. Solanum xanthocarpum exhibits better antibacterial properties on comparison to other two extracts.Solanum nigrum and Helianthus annuus exhibit better antifungal properties by being sensitive factor towards fungal medium. The obtained images were processed using color coding techniques to determine the activity of the extract by isolating the region of inhibition area. The region of inhibition was measured using matlab code and tabulation was compiled to compare the manually measured distances to the automated measurements. Conclusions:The results provided evidence that the studied plant extracts might indeed be potential sources of natural antimicrobial agents and the introduction of an evaluation technique using image processing was shown to be suitable for the purpose of accurate measurements of zone of inhibition.

Melissa officinalis Essential Oil: Chemical Compositions, Antioxidant Potential, Total Phenolic Content and Antimicrobial Activity

“…These substances are generally found in multiple parts of the herbs, including leaves, roots, shoots, blossoms, flowers and barks. Many herbs have thus become the origins of major drugs and the pharmaceutical industries have become interested in these herbs as sources of bioactive factors [44][45][46]. Results of this study plainly revealed that the M. officinalis EO antibacterial activities on the growth of clinical and commercial strains causing infections.…”

The Comparison Among Antibacterial Activity of Mespilus germanica Extracts and Number of Common Therapeutic Antibiotics “In Vitro”

“…Many plant extracts owe their potency to the presence of substances such as tannins, phenolics, micro elements, essential oils, peptides, unsaturated long chain aldehydes, alkaloids and so on. These substances are usually found in various parts of the plants like roots, leaves, shoots and bark [18]. Many plants have therefore become sources of important drugs and the pharmaceutical industries have come to consider them as a source of bioactive agents that can be used in the preparation of synthetic medicine [19].…”

UJI AKTIVITAS ANTIBAKTERI FRAKSI N-HEKSAN, ETIL ASETAT dan AIR DAUN LEUNCA (Solanum nigrum L) TERHADAP BAKTERI (Staphylococus aureus dan Escherichia coli)

“…buah solanum memiliki kandungan metabolit sekunder yaitu alkaloid, saponin, tannin, flavonoid dan steroid. Menurut penelitian [5] menunjukkan bahwa (solanum nigrum L) memiliki aktivitas antibakteri meneliti bahwa ekstrak methanol dari biji Solanum nigrum L menunjukkan aktivitas antibakteri terhadap bakteri Salmonella thypi, Bacilus subtilis, Staphylococus aureus.…”