Comparative evaluation of antimicrobial effect of<i>Artemisia vulgaris</i>essential oils extracted from fresh and dried herb

Singh, Bhoj Raj; Singh, Vidya; Singh, Ripudaman; Toppo, Saroj; Haque, Nazrul; Ngullie, Ebibeni

doi:10.5958/j.0975-4261.4.2.003

Cited by 9 publications

(11 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, there was a significant difference in sensitivity of different bacteria to herbal drugs indicating that herbal drugs also kill bacteria selectively and are not broad spectra. Similar observations have also been made earlier and enteric bacteria are often reported more resistant than other bacteria to herbal drugs [7, 8, 14–16]. …”

Section: Discussionsupporting

confidence: 87%

Antimicrobial and Herbal Drug Resistance in Enteric Bacteria Isolated from Faecal Droppings of Common House Lizard/Gecko (Hemidactylus frenatus)

Singh

Ngullie

et al. 2013

International Journal of Microbiology

Self Cite

View full text Add to dashboard Cite

From 194 faecal dropping samples of common house geckos collected from offices (60), houses (88), integrated farm units (IFS,18) and hostels, guest houses, and dining rooms of different canteen/mess (HGM, 28), 326 bacterial isolates of enteric bacteria belonging to 17 genera and 34 species were detected. Escherichia coli were the most frequently (39) isolated followed by Citrobacter freundii (33), Klebsiella pneumonia (27), Salmonella indica (12), Enterobacter gergoviae (12), and Ent. agglomerans (11). Other important bacteria isolated from gecko droppings were Listonella damsela (2), Raoultella terrigena (3), S. salamae (2), S. houtenae (3), Edwardsiella tarda (4), Edwardsiella hoshinae (1), and Klebsiella oxytoca (2). Of the 223 isolates tested for antimicrobial drug sensitivity, 27 (12.1%) had multiple drug resistance (MDR). None of the salmonellae or edwardsiellae had MDR however, MDR strains were significantly more common among Escherichia spp. (P = 1.9 × 10−5) and isolates from IFS units (P = 3.58 × 10−23). The most effective herbal drug, Ageratum conyzoides extract, inhibited growth of only 27.8% of strains tested followed by ethanolic extract of Zanthoxylum rhetsa (13.9%), eucalyptus oil (5.4%), patchouli oil (5.4%), lemongrass oil (3.6%), and sandalwood oil (3.1%), and Artemisia vulgaris essential oil (3.1%).

show abstract

Section: Discussionsupporting

confidence: 87%

Antimicrobial and Herbal Drug Resistance in Enteric Bacteria Isolated from Faecal Droppings of Common House Lizard/Gecko (Hemidactylus frenatus)

Singh

Ngullie

et al. 2013

International Journal of Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Similar pattern of higher sensitivity of microbes of food (vegetable as well as animal origin) has also been reported earlier for essential oils of Artemisia vulgaris [25,26], lemon grass oil [24] indicating that variables responsible for persistence of microbes in animal system might be associated with herbal drug resistance or vice-versa, but needs more targeted research to lucidly understand.…”

Section: Discussionsupporting

confidence: 77%

“…Though A. conyzoides is usually not consumed by animals but in scarcity, it may be. Similar views have also been expressed earlier for higher resistance in bacterial isolates from such kinds of animals for A. vulgaris, lemon grass and citronella oil [21,22,[24][25][26].…”

Section: Discussionsupporting

confidence: 74%

“…In the present study, the most sensitive strains to ACME belonged to oxidase positive GPBs (62.5%) followed by oxidase negative GPBs (40.8%), oxidase positive GNBs (27.4%) and oxidase negative GNBs (4.9%). The role of oxidase production ability might be important in herbal drug resistance, similar observation have been made earlier with lemongrass oil [24], Artemesia vulgaris oil [25,26], geranium oil [21] and citronella oil [22].…”

Section: Discussionsupporting

confidence: 71%

See 1 more Smart Citation

Antimicrobial Activity of Methanolic Extract and Ether Extract of Ageratum conyzoides

Singh¹

2015

Pharm Anal Acta

View full text Add to dashboard Cite

Ageratum conyzoides, a weed prevalent in India, is known for its several therapeutic uses to control infections. In the present study we compared the antimicrobial potential of its ether extract and methanolic extract with ciprofloxacin on 294 strains of Gram positive bacteria (GPBs), 575 strains of Gram negative bacteria (GNBs), 15 yeast and 5 mould strains of clinical and nonclinical origin belonging to 49 genera and more than 155 species using disc diffusion assay. The microbial strains in the study were isolated from samples of abiotic (41) and biotic (101) environment, foods (81), clinically sick (441), dead (108) and healthy (75) animals and human beings, and 42 were reference strains. The study revealed that there was no appreciable difference in antimicrobial activity of ether extract (ACEE) or methanolic extract (ACME) of A. conyzoides. A total of 214 (24.1%) strains were sensitive to ACME while of the 697 strains tested for ciprofloxacin 551 (79.1%) were sensitive. Sensitivity to ACME among 294 GPBs (44.9%) was significantly (p<0.0001) higher than among 575 strains of GNBs (12.4%). There was no significant difference among GPBs and GNBs for ciprofloxacin (one of the most commonly used antibiotics in India) sensitivity, but oxidase negative GNBs (385) as well as GPBs (238) were about two times more commonly sensitive to ciprofloxacin than 190 oxidase positive GNBs (p = 0.001) and 56 oxidase positive GPBs (p, 0.03), respectively. For ACME oxidase positive strains had 2.4 times more odds (p < 0.0001) in their favour of being sensitive to ACME (53.4%) than oxidase negative strains (18.6%). The most sensitive strains to ACME belonged to oxidase positive GPBs (62.5%) followed by oxidase negative GPBs (40.8%), oxidase positive GNBs (27.4%) and oxidase negative GNBs (4.9%). All Aeromonas, Alcaligenes, Klebsiella, and Proteus species strains were resistant to ACME irrespective of source of isolation or association with illness. In contrast, majority of the strains of Burkholderia (76.9%), Bacillus (66.7%) and Brucella (53.8%) species were sensitive to ACME. The study revealed that A. conyzoides might be containing useful antimicrobial component(s) more active against oxidase positive potentially pathogenic strains often associated with systemic and deadly infections in animals as well as in humans.

show abstract

“…However, earlier studies have not made similar comparison; higher sensitivity of Axone origin strains than strains of animal origin has been reported [15][16][17][18]. Therefore, it might be attributed to carryover effect on resistant strains of bacteria from animals to their products and then to the food handlers or transfer of drug resistant bacteria from hands of food handlers to animal products [19,20].…”

Section: Effect Of Herbal Antimicrobials On Bacterial Strains Of Foodmentioning

confidence: 96%

Effect of Herbal Antimicrobials on Bacterial Strains of Foods of Vegetable and Animal Origin

Singh¹,

Sinha²,

Kumar³

2016

J Food Chem Nanotechnol

View full text Add to dashboard Cite

At the face of emergence of multiple antibiotic resistant strains, herbal antimicrobials are looked as the future drugs for therapeutics, and also as food preservative. This study was undertaken to understand the antimicrobial activity of some selected herbal preparations on bacteria of food origin. In the study 464 (254 from foods of vegetable origin, 134 from foods of animal origin, 14 from food handlers, 62 reference) strains belonging to more than 104 species of 33 genera of Gram negative (194) bacteria (GNB) and Gram positive (270) bacteria (GPB) were tested for their sensitivity to methanolic extract of Eupatorium odoratum (EOME), methanolic extract of Ageratum conyzoides (ACME), methanolic extract of Zanthoxylum rhetsa seed coat (ZRME), lemon grass (Cymbopogon citratus) oil (LGO), sandalwood (Santalum album) oil (SWO), patchouli (Pogostemon cablin) oil (PO) and agarwood (Aquilaria crassna) oil (AO) through disc diffusion method. Besides, minimum inhibitory concentration (MIC) of different herbal preparations was determined for test strains using agar-well dilution method. Sandalwood oil inhibited maximum number of strains (50.5%) followed by LGO (45.9%), EOME (43.8%), ACME (40.4%), PO (38%), ZEME (19.6%) and AO (17.5%). Enterobacteriaceae strains in general showed more resistance to herbal antimicrobials than strains of any other family. The MIC for different herbal preparations varied for different strains from 1 µg mL -1 to > 16.384 mg mL -1 . The study indicated that herbal antimicrobials being edible and acceptable since ages can be an option for use in foods to control spoilage and choice may depend on type of microbe causing the problem in the specific food unit.

show abstract

Comparative evaluation of antimicrobial effect ofArtemisia vulgarisessential oils extracted from fresh and dried herb

Cited by 9 publications

References 0 publications

Antimicrobial and Herbal Drug Resistance in Enteric Bacteria Isolated from Faecal Droppings of Common House Lizard/Gecko (Hemidactylus frenatus)

Antimicrobial and Herbal Drug Resistance in Enteric Bacteria Isolated from Faecal Droppings of Common House Lizard/Gecko (Hemidactylus frenatus)

Antimicrobial Activity of Methanolic Extract and Ether Extract of Ageratum conyzoides

Effect of Herbal Antimicrobials on Bacterial Strains of Foods of Vegetable and Animal Origin

Contact Info

Product

Resources

About