Comparative evaluation of antimicrobial efficacy on Enterococcus faecalis and smear layer removal in curved canals by different irrigation techniques: An in vitro study

Nair, RevathyM; Jayasree, S; Poornima, ESruthi; Ashique, M

doi:10.4103/jcd.jcd_224_22

Cited by 4 publications

(1 citation statement)

References 19 publications

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“…concluded that Er,Cr:YSGG laser-assisted irrigation offered better biofilm elimination in the apical root portions compared to syringe and ultrasonic irrigant agitation techniques. [ 19 20 ]…”

Section: Discussionmentioning

confidence: 99%

Effect of ultrasonic and Er,Cr:YSGG laser-activated irrigation protocol on dual-species root canal biofilm removal: An in vitro study

Datla,

Uppalapati,

Pilli

et al. 2024

Journal of Conservative Dentistry and Endodontics

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Aim: The aim of the study was to investigate the disinfecting efficacy of a standardized irrigating solution activated by ultrasonics or laser irradiation on mature dual-species biofilms at different root levels in vitro. Materials and Methods: Conventional access cavity preparations were done on 160 single-rooted mandibular premolar teeth with single canals. Freshly extracted oral microbial strains of Staphylococcus aureus, Streptococcus mutans, Enterococcus faecalis, and Candida albicans after biochemical confirmation were used to generate two discrete dual-species microbial inoculums. The sterilized tooth samples were randomly segregated into two groups (n = 80) and inoculated with a mixed inoculum of S. aureus + E. faecalis strains (Group 1) and S. mutans + C. albicans strains (Group 2), respectively. Following the 21-day incubation period under aerobic conditions, the infected specimens in each group were divided into four subgroups (n = 20) and subjected to experimental treatment protocols. This included a positive control (no treatment of biofilms), syringe irrigation alone with TruNatomy needle, passive ultrasonically activated irrigation with 20# Irrisafe tip, and laser agitation of irrigant with Er,Cr:YSGG laser using RFT 2 laser tip. Root canals of experimental specimens (except the control samples) are instrumented with TruNatomy rotary file system using 1:1 mixture of 3% NaOCl and 18% etidronic acid as irrigants. The quantitative assessment of reduction in viable biofilm microbes after treatment was done using colony-forming unit counts and confocal laser scanning microscopy image analysis. The obtained data were analyzed statistically with a significant level set at 0.05. Results: Laser-assisted irrigation has shown a considerably higher mean percentage reduction of microbes compared to ultrasonic agitation and the syringe irrigation showed the least microbial reduction (P = 0.001). No significant difference was noted between the three root regions of ultrasonic and laser groups (P > 0.05), whereas in the syringe groups, apical portions showed higher microbial counts compared to cervical and mid-root regions (P = 0.001). Conclusion: Erbium laser-assisted irrigation has performed superior to ultrasonic agitation against both the experimental dual-species biofilms, while the syringe irrigation showed the least microbial reduction specifically at apical root portions.

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Section: Discussionmentioning

confidence: 99%

Effect of ultrasonic and Er,Cr:YSGG laser-activated irrigation protocol on dual-species root canal biofilm removal: An in vitro study

Datla,

Uppalapati,

Pilli

et al. 2024

Journal of Conservative Dentistry and Endodontics

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Antibacterial Effect of Silver Nanoparticles on Polymicrobial Biofilms in Endodontics: Systematic Review and Meta Analysis

Olivares-Acosta,

Romero-Sánchez,

Marín

et al. 2024

Journal of Nanotechnology

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This systematic review investigates the antibacterial effects of silver nanoparticles (AgNPs) on polymicrobial biofilms in endodontics, emphasizing their potential enhancement of traditional treatment methods. Recognizing the limitations of current endodontic disinfectants in fully eradicating complex biofilms, this study focuses on the synergistic potential of AgNPs to improve antimicrobial efficacy. Utilizing a systematic approach based on PRISMA guidelines, the review draws on studies from databases like PubMed, Web of Science, and SCOPUS up to 2024. The included research specifically examines the role of AgNPs in various endodontic applications against resilient biofilms, adhering to strict inclusion criteria that demand clear methodology and measurable outcomes. The results suggest that AgNPs can significantly augment the biofilm disruption capabilities of conventional antimicrobials, potentially due to their ability to modify the biofilm matrix and enhance agent penetration. However, the effectiveness varies depending on the nanoparticle concentration and application technique, indicating a need for further optimized studies. In conclusion, while AgNPs show promise in enhancing endodontic antimicrobial protocols, additional in vivo research is necessary to establish standardized, effective, and safe clinical applications. The review highlights the complex interactions between nanoparticle properties and biofilm biology, underscoring the importance of further investigation in this area.

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Photon-Induced Photo-Acoustic Streaming vs. Shock Wave-Enhanced Emission Photo-Acoustic Streaming—The Effect of Three Final Irrigation Protocols on the Bond Strength of an Individually Formed Fiber Post

Lupita,

Bojoga,

Del Vecchio

et al. 2024

Dentistry Journal

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(1) Background: This study aimed to evaluate how laser-activated irrigation (LAI) influences the retention of a fiber post when used before an endodontic filling, as well as after post space preparation. (2) Materials and Methods: Sixty freshly extracted human incisors were selected. The teeth were randomly assigned to three groups—CONVENTIONAL (CONV), PIPS or SWEEPS—and treated endodontically. Each group received irrigation with 1 × 5 mL EDTA (17%) and 3 × 5 mL NaOCl (5.25%). In the first group, the irrigants were not activated, while in the second and third group, LAI was adopted using PIPS and SWEEPS protocols (Lightwalker from Fotona, Ljubliana, Slovenia). After post space preparation, each group received the same irrigation protocol initially established. Sticky posts (everStick Post, GC AUSTRIA GmbH Swiss) were individually adapted to the corresponding post spaces and cemented using dual cure resin cement (Gradia Core, GC Austria GmbH Swiss). All specimens were vertically embedded into self-curing acrylate (Duracryl plus, Spofa Dent, Europe), and each was sectioned into three segments of type A and type B samples for debonding through push-out and pull-out tests. The results were statistically analyzed. (3) Results: The pull-out test showed the superiority of the SWEEPS group, with a mean fracture force of 133.0 ± 50.7 N, followed by the PIPS group, with 102 N, with a lower standard deviation of ± 34.5 N. The CONV group registered the lowest fracture force. Concerning the push-out test, the SWEEPS group showed superior shear stress in comparison to the other two groups (13.45 ± 4.29 MPa); the CONV group was inferior, with shear tension values of 8.31 ± 4.67 MPa. (4) Conclusions: It can be stated that the SWEEPS and PIPS protocols resulted in considerably higher fiber post retention than the conventional method, whereas the SWEEPS protocol was superior to the PIPS protocol.

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Comparative evaluation of antimicrobial efficacy on Enterococcus faecalis and smear layer removal in curved canals by different irrigation techniques: An in vitro study

Cited by 4 publications

References 19 publications

Effect of ultrasonic and Er,Cr:YSGG laser-activated irrigation protocol on dual-species root canal biofilm removal: An in vitro study

Effect of ultrasonic and Er,Cr:YSGG laser-activated irrigation protocol on dual-species root canal biofilm removal: An in vitro study

Antibacterial Effect of Silver Nanoparticles on Polymicrobial Biofilms in Endodontics: Systematic Review and Meta Analysis

Photon-Induced Photo-Acoustic Streaming vs. Shock Wave-Enhanced Emission Photo-Acoustic Streaming—The Effect of Three Final Irrigation Protocols on the Bond Strength of an Individually Formed Fiber Post

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