Comparative evaluation of bacterial cellulose (nata) as a cryoprotectant and carrier support during the freeze drying process of probiotic lactic acid bacteria

Production for High-vitality Starter Culture of <i>Lactobacillus plantarum</i> NDC 75017 by High Cell-density Cultivation and Low-temperature Vacuum Drying

“…While Fig.6b illustrated that lyophilized cells were tightly aggregating in large cell clusters and the surface of cells shrank when 0.85% physiological saline was used as sole cryoprotectant. Similar results had been reported by Jagannath (2010), which showed that water and saline were poor cryoprotectants. However, in the presence of optimal cryoprotectants (Fig.…”

“…The number of viable counts decreased after lowtemperature vacuum drying, and a similar result was obtained by Jagannath et al (2010), who reported higher cell losses with 2 _ 3 log cycle reductions after low-temperature vacuum drying in the CFU compared with the original cell suspension in the case of all lactic acid bacteria. However, the level of cell viability after lowtemperature vacuum drying varied according to numerous factors including the strain and the efficacy of the protective agents used (Edward, et al, 2011;Hongpattarakere, et al, 2013;Jagannath, et al, 2010;Vinderola, et al, 2012). According to the orthogonal experimental design methodology (Shown in the microorganism proteins to improve the stabilisation of protein structure; (b) the ability of retaining amounts of residue moisture was improved during freeze-drying.…”

Hypothermia protection effect of antifreeze peptides from pigskin collagen on freeze-dried Streptococcus thermophiles and its possible action mechanism

“…We observed that the APP improved the viability more effectively than the commercial cryoprotectants used in this study. In comparison, similar viabilities have been reported that the initial cell suspension of individual lactic acid bacteria contained 10 9 e10 10 CFU/g which decreased to 10 7 CFU/g immediately after the freeze drying process, with 67e70 % of viability treated with 10% of skim milk (Jagannath, Raju, & Bawa, 2010). The viability of freeze dried Geotrichum candidum (G. candidum) was about 41% immediately after the freeze drying process in the presence of sucrose at 23% (w/v) (Hamoudi, Goulet, & Ratti, 2007).…”

Poly-γ-glutamic acid: production, properties and applications

“…c-PGA: production, properties and applications Lactobacillus paracasei significantly better than 10 % sucrose, which has been reported previously to offer better protection during freeze-drying of lactobacilli compared with trehalose and sorbitol (Siaterlis et al, 2009), and nata, a bacterial cellulose produced by Acetobacter xylinum (Jagannath et al, 2010). Lim et al (2012) investigated the effect of c-PGA on oil absorption and moisture loss in doughnuts during deep-fat frying.…”