Comparative Evaluation of Different Test Combinations for Diagnosis of<i>Mycobacterium avium</i>Subspecies<i>paratuberculosis</i>Infecting Dairy Herds in India

Garg, Rajni; Patil, Prasanna Kumar; Singh, Shoor Vir; Sharma, Shukriti; Gandham, Ravi Kumar; Singh, Ajay Vir; Filia, Gursimran; Singh, Pravin Kumar; Jayaraman, Sujatha; Gupta, Saurabh; Chaubey, Kundan Kumar; Tiwari, Ruchi; Saminathan, Mani; Dhama, Kuldeep; Sohal, Jagdip Singh

doi:10.1155/2015/983978

Cited by 22 publications

(17 citation statements)

References 39 publications

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“…The present findings were in agreement with Garg et al (2015) who also reported good agreement between serum ELISA and faecal PCR and fair agreement between microscopy and faecal PCR in dairy animals. Whereas, Singh et al (2013) reported substantial agreement between "microscopy" and blood PCR.…”

Section: Advances In Animal and Veterinary Sciencessupporting

confidence: 93%

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Prevalence of Ovine Johnes Disease in Bannur Breed of Sheep in Organized Farm using Multiple Diagnostic Tests

Mukartal¹,

Rathnamma²,

Narayanaswamy³

et al. 2016

Adv. Anim. Vet. Sci.

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Section: Advances In Animal and Veterinary Sciencessupporting

confidence: 93%

“…Sensitivity depends on the stages of disease within the flock and the antigens used in the serologic test (Hilbink et al, 1994). Present findings were in accordance with Garg et al (2015) who also reported higher sensitivity of ELISA-PCR compared to microscopy-PCR.…”

Section: Advances In Animal and Veterinary Sciencessupporting

confidence: 90%

Prevalence of Ovine Johnes Disease in Bannur Breed of Sheep in Organized Farm using Multiple Diagnostic Tests

Mukartal¹,

Rathnamma²,

Narayanaswamy³

et al. 2016

Adv. Anim. Vet. Sci.

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“…In another study, sensitivity and specificity of indigenous ELISA kit was 66.6 and 75.0% and 68.1 and 66.6% with tissue culture and tissue PCR, respectively (Singh et al, 2008). For estimating sensitivity and specificity of ELISA in case of MAP infection is by comparing serum ELISA with fecal culture (Garg et al, 2015). Similar comparative studies were carried out by Singh et al (2009b), where 'Indigenous g-ELISA kit' had superior diagnostic potential and high specificity as compared to commercial ELISA kit.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic Potential of Three Antigens from Geographically Different Regions of the World for the Diagnosis of Ovine Johne’s Disease in India

Chaubey¹,

Singh²,

Gupta³

et al. 2015

Asian J. of Animal and Veterinary Advances

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Cross reactivity of three antigens of Mycobacterium avium subspecies paratuberculosis with sera of sheep endemic for Johne's disease was evaluated. Out of 40 sheep tested by fecal microscopy, 72.5% were shedding MAP. Using protoplasmic antigens (PPA) from three MAP strains isolated from different livestock species and geographical regions, 90, 77.5 and 2.5% sheep were positive in goat (Indigenous g-ELISA) and cattle (b-ELISA) based ELISA kits and ELISA kit for small ruminant (sr-ELISA), respectively. Only 2.5 and 10% sheep were positive and negative in all the four tests. Native species specific (goat origin novel 'Indian Bison Type' MAP) semi-purified whole cell PPA based ELISA (Indigenous g-ELISA) was superior in reacting with sera of native sheep than the commercial PPA of bovine origin (Allied Monitor Inc., USA) and commercial ELISA kit for small ruminants (ID Vet, France). Lower cross reactivity of antigens originated from US and France emphasized the need to develop tests based on local strain of MAP than strains from different livestock species and geographical regions. This is an important finding against the use of 'Global kits' without validating in local conditions. Study showed that kits developed from local strains of MAP were not only superior but also cost effective and will significantly contribute in programs for the control of JD in native sheep population.

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“…An AC electrokinetic impedance sensing can be used for rapid and sensitive detection of specific antibodies in serum samples and this could be a basis for development of a point of care diagnostic device for human and bovine tuberculosis (Cui et al, 2013). Garg et al (2015) recently reported that the combination of milk ELISA and milk PCR may be adopted as a model strategy for the screening and diagnosis of JD in lactating cattle herds in Indian conditions.…”

Section: Discussionmentioning

confidence: 99%

Immunoreactivity to Culture Filtrate Proteins of Mycobacterium avium Subspecies paratuberculosis in Naturally Infected Goat and Sheep Sera

Gupta¹

2015

Adv. Anim. Vet. Sci.

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INTRODUCTIONM ycobacterium avium subspecies paratuberculosis (MAP), a cause of Johne's disease (Paratuberculosis) is the most wide-spread and highly prevalent due to difficulties in diagnosing pre-clinical cases. MAP has wide host range including domestic and wild ruminants, free grazing animals and also the human beings (Singh et al., 2010;Singh et al., 2014a;Singh et al., 2014b). Paratuberculosis has high economic impact on dairy industry and > USD 250 million economic losses has been reported in US alone (Ott et al., 1999). Though disease is endemic in India and economic losses in dairy farm was estimated from reproductive disorders (Rs. 23400.0/cow/year), forced removal (Rs. 41,750.0/cow/year), reduced milk yield (Rs. 5,712.0/cow/ year) and increased mortality (Rs. 11,666.0/ cow/year) (Rawat et al., 2014). Control of paratuberculosis has been hindered due to lack of efficient and accurate diagnostic tests. Lower specificity of tests can also be a problem due to the sharing of antigens or epitopes among MAP and other mycobacteria (Collins et al., 1991). Sensitivity of commercially available ELISA kits prepared using protoplasmic antigen has been reported comparatively low, 13.6-33.3% (Singh et al., 2007).Recent studies have focused on developing improved serodiagnostics using species-specific multiple protein antigens (Singh et al., 2014c). Microfluidics and Lab-on-Chip are some of the recent technologies that can predicate development of laboratory-free diagnostic devices for Research ArticleAbstract | Mycobacterium avium subspecies paratuberculosis (MAP), the cause of granulomatous chronic enteritis in ruminants ( Johne's disease) is under reported due to difficulties in diagnosing pre-clinical cases. Compromised specificity is a problem due to extensive sharing of antigens /epitopes among MAP and other mycobacterial strains. Culture filtrate (CF) proteins profile of native 'Indian Bison Type' strain of MAP was studied at different harvesting times (2-8 weeks of growth) in Middlebrook 7H9 medium supplemented with ADC, PANTA antibiotics and mycobactin J. Analysis of harvested CF proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed that the greater part of CF proteins had molecular masses (<70 kDa) as 14, 19, 26, 34-41, 52-55, and 70 kDa. Immunoblotting showed reactivity of CF proteins commonly recognised (28, 34-36, 38-42, 45, and 56 kDa) with all four MAP infected goat and sheep sera at 2-8 weeks of growth. Collectively, these immunoreactive MAP CF proteins could be the potential targets for developing diagnostics against Johne's disease with improved sensitivity and high specificity instead of whole cell sonicated crude protoplasmic extracts (PPA). Keywords

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Comparative Evaluation of Different Test Combinations for Diagnosis ofMycobacterium aviumSubspeciesparatuberculosisInfecting Dairy Herds in India

Cited by 22 publications

References 39 publications

Prevalence of Ovine Johnes Disease in Bannur Breed of Sheep in Organized Farm using Multiple Diagnostic Tests

Prevalence of Ovine Johnes Disease in Bannur Breed of Sheep in Organized Farm using Multiple Diagnostic Tests

Diagnostic Potential of Three Antigens from Geographically Different Regions of the World for the Diagnosis of Ovine Johne’s Disease in India

Immunoreactivity to Culture Filtrate Proteins of Mycobacterium avium Subspecies paratuberculosis in Naturally Infected Goat and Sheep Sera

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