Background: Patients often complain about the esthetic problems of gingival pigmentation. The aim of this study was to compare the effectiveness and recurrence of diode laser 810 nm and cryosurgery for gingival depigmentation. Methods: A total of 12 patients with physiologic gingival pigmentation were selected. The inclusion criterion was the presence of gingival pigmentation of maxillary incisors and the exclusion criteria was the presence of systemic diseases or use of any drugs associated with gingival pigmentation. For further evaluation, photographs were taken before and after treatment within the same position and light. Color analysis was done using the Adobe Photoshop software. Patients were treated by diode laser 810 nm -2 w and on the opposite side cryosurgery was performed. Treatment was done under topical anesthesia if required. The laser beam was activated using the brush technique and in contact mode. Application period differed according to the degree of melanin pigmentation and epithelial thickness of the treated area. Cryosurgery was performed by a cotton swab. The swab was gently touched and rolled on the area for 10 to 15 seconds until the disappearance of gingival pigmentation. All patients were examined 1, 3, and 6 months after treatment. Results: Data were analyzed using the SAS software version 19. The degree of pigmentation was considered as follows: score1: mild pigmentation, score 2: average pigmentation, and score 3: intense pigmentation. The treatment of gingival pigmentation with laser and cryosurgery showed marked improvement of gingival pigmentation in both methods as well as no significant difference between the 2 methods after 1, 3, and 6 months of treatment. Conclusions: Both methods are appropriate for the treatment of gingival depigmentation and no significant recurrence was observed during the 6 months follow -up.