Comparative evaluation of immunoperoxidase versus immunofluorescent techniques in interpretation of kidney biopsies

Shubham, Swasti; Bhardwaj, Minakshi; Mahapatra, Himansu Sekhar

doi:10.4103/0377-4929.188105

Cited by 5 publications

(1 citation statement)

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“…Histological evaluation using light microscopy (LM), immunohistochemistry, and electron microscopy (EM) is usually required for the interpretation of medical renal biopsies [ 1 ]. For more than 50 years, immunofluorescence on frozen tissue (IF–F), which was first used by Coons and his colleagues in 1942 [ 2 ], has been the best immunohistochemical method for finding immunoglobulins (Igs) and complement components in the kidney.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Accuracy of Direct Immunofluorescence Test on Paraffin-Embedded Blocks in Comparison with Frozen Section Blocks in Renal Biopsies

Mohammadzadeh

Aghakhaninejad

Azad

et al. 2022

International Journal of Nephrology

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Background. In several published research, the evaluation of renal disorders using immunofluorescence on formalin-fixed, paraffin-embedded (FFPE) tissue sections versus immunofluorescence on frozen sections was compared. Each technique’s accuracy varies greatly. This study’s objective was to assess IF-P as a potential replacement for IF-F in the diagnosis of renal biopsy specimens. Materials and Methods. To show immunoglobulin IgA, IgG, IgM, and C3 immune deposits, proteinase K digestion of paraffin-embedded renal biopsy was standardized and used in 51 renal biopsies. Sensitivity, specificity, false-positive, and false-negative values were calculated. Results. IF-P showed a sensitivity of 93.1%, 76.9%, 63.6%, and 33.3%, and a specificity of 100%, 97.3%, 95%, and 100% for IgG, IgA, IgM, and C3, respectively. Compared to cases that had both routine IF and IF-P, 50 of 51 showed either the same amount of staining for the diagnostic immunoglobulin/complement or a small amount of difference. In most of the cases (49 of 51), diagnostic findings were found. Conclusion. IF-P is a sensitive and precise approach for assessing immune deposits in renal tissue biopsies. We come to the conclusion that IF-P serves as a beneficial salvage immunohistochemistry method for renal biopsies that do not contain enough cortical tissue for IF-F.

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Section: Introductionmentioning

confidence: 99%