Comparative evaluation of inorganic and organic amendments for their flocculation efficiency of selected microalgae

Rakesh, Suchitra; Saxena, Shalini; Dhar, Dolly Wattal; Prasanna, Radha; Saxena, Anil Kumar

doi:10.1007/s10811-013-0114-4

Cited by 48 publications

(16 citation statements)

References 42 publications

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“…On the contrary, no flocculation or settling was found for Chlorella zofingiensis cultures in the pH range of 1.82-11.97 (Wyatt et al, 2012). Therefore, harvesting of microalgae biomass using pH adjustment could be strain specific (Rakesh et al, 2013). Additionally, adjustment of culture pH to extreme level may require excess amount of chemicals as found in this study; similar amount of chemicals would be necessary to neutralize the supernatant.…”

Section: Comparison With High and Low Ph Induced Coagulationflocculationmentioning

confidence: 61%

“…In many previous studies, ferric chloride was used effectively to harvest different microalgae biomass (Rakesh et al, 2013;Seo et al, 2015;Wyatt et al, 2012). However, a downside effect of ferric chloride (or other coagulant) based microalgae harvesting is that a major fraction of the coagulant is expected to be associated with the microalgae biomass.…”

Section: Introductionmentioning

confidence: 99%

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Microalgae harvesting by pH adjusted coagulation-flocculation, recycling of the coagulant and the growth media

Das

Thaher

Hakim

et al. 2016

Bioresource Technology

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Section: Comparison With High and Low Ph Induced Coagulationflocculationmentioning

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Microalgae harvesting by pH adjusted coagulation-flocculation, recycling of the coagulant and the growth media

Das

Thaher

Hakim

et al. 2016

Bioresource Technology

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“…urea, single super phosphate, and muriate of potash) as given in Rakesh et al. . Batch cultures were maintained by inoculating a known volume of fresh medium every two weeks for use as seed culture.…”

Section: Methodsmentioning

confidence: 99%

“…These cultures were grown in trays under greenhouse conditions of 25˚C temperature and natural sunlight (60.8-67.5 μmol photons m −2 s −1 ) in formulated commercial medium containing NPK fertilizers (i.e. urea, single super phosphate, and muriate of potash) as given in Rakesh et al [10]. Batch cultures were maintained by inoculating a known volume of fresh medium every two weeks for use as seed culture.…”

Section: Methodsmentioning

confidence: 99%

Cell disruption methods for improving lipid extraction efficiency in unicellular microalgae

Rakesh

Dhar

Prasanna

et al. 2015

Engineering in Life Sciences

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Identification of cost‐effective cell disruption methods to facilitate lipid extraction from microalgae represents a crucial step in identifying promising biofuel‐producing species. Various cell disruption methods including autoclaving, microwave, osmotic shock, and pasteurization were tested in the microalgae Chlorococcum sp. MCC30, Botryococcus sp. MCC31, Botryococcus sp. MCC32, and Chlorella sorokiniana MIC‐G5. Lipid content (on dry weight basis) from the four cultures on day 7 ranged from 11.15 to 48.33%, and on day 14 from 11.42 to 44.26%. Among the methods tested, enhanced lipid extraction was achieved through osmotic shock (15% NaCl) for Botryococcus sp. MCC32, microwave (6 min) for Botryococcus sp. MCC31, osmotic shock (5% NaCl) for Chlorella sorokiniana MIC‐G5 and microwave (2 min) for Chlorococcum sp. MCC30. The highest palmitate (16:0) contents (25.64% and 34.20%) were recorded with osmotic shock (15% NaCl) treatment for Botryococcus sp. MCC32 and microwave (6 min) for Botryococcus sp. MCC31, respectively. Two strains, along with their respective cell disruption methods, were identified as promising oil blends or nutraceuticals due to their high unsaturated fatty acid (UFA) content: Botryococcus sp. MCC31 (37.6% oleic acid content; 39.37% UFA) after autoclaving and Botryococcus sp. MCC32 after osmotic shock of 15% NaCl treatment (19.95% oleic acid content; 38.17% UFA).

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“…Examples of natural positively charged natural flocculants are poly-γ-glutamic acid, a polymer produced by Bacillus subtilis (Zheng et al 2012), and flour from the seeds of the Moringa oleifera tree (Teixeira et al 2012). Some studies suggest that uncharged natural polymers such as starch may also be capable of inducing flocculation in microalgae (Rakesh et al 2013). Uncharged natural biopolymers may be modified with cationic functional groups to improve flocculation efficiency.…”

Section: Polymer Flocculationmentioning

confidence: 99%

Harvesting of Microalgae by Means of Flocculation

Muylaert

Vandamme

Foubert

et al. 2015

Biofuel and Biorefinery Technologies

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Large-scale production of microalgae for biofuels is still facing several major challenges to become competitive with other forms of renewable and nonrenewable energy. A major challenge is harvesting, which requires the separation of a low amount of biomass consisting of small individual cells from a large volume of culture medium. Flocculation is seen as a promising low-cost harvesting method for microalgae biomass. In this chapter, the challenges and potential advantages of using flocculation as a harvesting method for microalgae are reviewed.

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Comparative evaluation of inorganic and organic amendments for their flocculation efficiency of selected microalgae

Cited by 48 publications

References 42 publications

Microalgae harvesting by pH adjusted coagulation-flocculation, recycling of the coagulant and the growth media

Microalgae harvesting by pH adjusted coagulation-flocculation, recycling of the coagulant and the growth media

Cell disruption methods for improving lipid extraction efficiency in unicellular microalgae

Harvesting of Microalgae by Means of Flocculation

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