Comparative Evaluation of Lipofectamine and Dendrimer for Transfection of Short RNA Into Human T47D and MCF-10A Cell Lines

Jahanafrooz, Zohreh; Bakhshandeh, Behnaz; Shirzadi, Erfan

doi:10.34172/apb.2023.022

Cited by 5 publications

(2 citation statements)

References 34 publications

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“…Comparing this figure with silibinin, a principal active constituent of the SM, is encouraging. Our findings accord with previous studies, which suggested that silibinin and SM nanoformulation could be potent inducers of BAX and inhibitors of the BCL2 gene expression. ,− BAX / BCL2 ratio was increased after the SW480 cell treatment with the SM–tin(IV) complex. Proapoptotic BAX to antiapoptotic BCL2 is a crucial hallmark of cell susceptibility to apoptosis. − It seems that the alterations above in the expression of BAX and BCL2 go hand in hand to sensitize cells to death.…”

Section: Discussionsupporting

confidence: 92%

Silymarin-Loaded Tin(IV) Nanoparticles Exhibit Enhanced Bioavailability and Antiproliferative Effects on Colorectal Cancer Cells

Abbasinia,

Heshmati,

Yousefi

et al. 2023

ACS Appl. Bio Mater.

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Silymarin (SM) exhibits potential therapeutic effects due to having antioxidant activity. However, the low solubility and bioavailability of SM restrict its biological performance. To overcome this limitation, this study aimed to develop a nanoformulation composed of SM and dimethyltindichloride and investigate the effect of SM-loaded Sn nanoparticles on cancer cell growth and survival. An SM−Sn complex was synthesized and then characterized using X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FTIR), EDS-MAP, dynamic light scattering (DLS), and ζ-potential analysis. After that, the SW480 colorectal cancer cell line was treated with different concentrations of SM and the SM−Sn complex. Cell viability was examined through the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, analyzing apoptosis, and live−dead assay. The lipid peroxidation rate was assessed through the measurement of thiobarbituric acid (TBA). Intracellular reactive oxygen species (ROS) level and cell population in the cell cycle were measured using a flow cytometry instrument. To evaluate the colonization ability of SW480 cells, a colony formation assay was performed. Gene expression analysis was also conducted using a real-time polymerase chain reaction (PCR) technique. The findings of this study revealed the effectiveness of the SM−Sn complex in decreasing SW480 cell viability by inducing cell death-associated mechanisms. We found that the SM−Sn complex increases intracellular ROS level and malondialdehyde (MDA) content. It was also revealed that the SM−Sn complex induces cell cycle arrest and the expression of apoptotic genes. In addition, the SM−Sn complex could effectively hinder SW480 cells from constituting colonies. We conclude that the use of tin(IV) as a scaffold for enhanced delivery of SM could be considered an efficient option for inhibiting cancer cell proliferation and survival.

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Section: Discussionsupporting

confidence: 92%

Silymarin-Loaded Tin(IV) Nanoparticles Exhibit Enhanced Bioavailability and Antiproliferative Effects on Colorectal Cancer Cells

Abbasinia,

Heshmati,

Yousefi

et al. 2023

ACS Appl. Bio Mater.

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“…Many studies have reported the inhibition of proliferation and cell cycle progression by silibinin in numerous cancers (6,7). It has also been reported that silibinin alone can induce apoptosis and cell cycle arrest in the paclitaxel-resistant ovarian carcinoma cell line and sensitize these cells to paclitaxel (8)(9)(10)(11)(12)(13). Silibinin is well-tolerated by the body and has proven less toxic than other anticancer therapeutics (7).…”

Section: Introductionmentioning

confidence: 99%

Silibinin Induces Apoptosis and G2/M Cell Cycle Arrest in Human Ovarian Cancer SKOV-3 Cell Line

Baghal Sadriforoush,

Jahanafrooz,

Motamed

2023

Jentashapir J Cell Mol Biol

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Background: Silibinin, an herbal polyphenolic flavonolignan, has antioxidant and anticancer properties. Objectives: This study aimed to evaluate some cellular and molecular effects of silibinin on the human ovarian cancer SKOV-3 cell line. Methods: For cytotoxicity investigations of silibinin, MTT assay was used at 24, 48, and 72 hours. Apoptosis and cell cycle were studied by flow cytometry. The effect of silibinin on mRNA expression of B-cell lymphoma 2 (Bcl-2), cyclin E, and S-phase kinase-associated protein 2 (SKP2) was determined by Quantitative reverse transcription polymerase chain reaction (QRT-PCR). Results: Silibinin administration in lower concentrations (12.5 and 25 µg/mL) did not lead to significant (P < 0.05) changes in cell viability and even slightly increased cell growth after 72 hours. However, silibinin in higher concentrations (≥ 50µg/mL) inhibited SKOV-3 cell proliferation in a dose- and time-dependent manner. The mode of cell growth inhibition was apoptosis induction and G2/M cell cycle arrest. Silibinin caused down-regulation of the anti-apoptotic gene, namely Bcl-2. Additionally, silibinin resulted in down-regulation of the major genes in the cell cycle, including cyclin E and SKP2. Conclusions: Overall, this study confirmed the ability of silibinin to suppress ovarian cancer progression through the induction of apoptosis and inhibition of G2/M phase transition. Silibinin may be considered an efficient and safe herbal medication for ovarian cancer.

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Recent Trends in the Application of Materials for Cancer Therapy and Diagnosis

Bakhshandeh,

Jahanafrooz,

Haghighi

et al. 2023

Interaction of Nanomaterials With Living Cells

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Comparative Evaluation of Lipofectamine and Dendrimer for Transfection of Short RNA Into Human T47D and MCF-10A Cell Lines

Cited by 5 publications

References 34 publications

Silymarin-Loaded Tin(IV) Nanoparticles Exhibit Enhanced Bioavailability and Antiproliferative Effects on Colorectal Cancer Cells

Silymarin-Loaded Tin(IV) Nanoparticles Exhibit Enhanced Bioavailability and Antiproliferative Effects on Colorectal Cancer Cells

Silibinin Induces Apoptosis and G2/M Cell Cycle Arrest in Human Ovarian Cancer SKOV-3 Cell Line

Recent Trends in the Application of Materials for Cancer Therapy and Diagnosis

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