Comparative evaluation of micronuclei in exfoliated oral epithelial cells in potentially malignant disorders and malignant lesions using special stains

Gupta, Neha; Rakshit, Apurva; Srivastava, Smriti; Suryawanshi, Hema; Kumar, Pramod; Naik, Rupali

doi:10.4103/jomfp.jomfp_164_17

Cited by 5 publications

(1 citation statement)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore the increase in MN was associated with genotoxic damage. 22 This event is related to the evidence found in a systematic review that supports the evaluation of MN in the oral epithelium as a biomarker of genotoxic damage since these MN would be a consequence of chromosomal instability caused by exposure to radiation and toxic chemical agents. 23 In this sense, our results could reflect a certain degree of genotoxicity in buccal epithelial cells related to work activity and open possibilities for the generation of future studies that assess not only the genotoxicity induced by artisanal mining activity but also its evaluation as a rapid, reliable, and valid effect biomarker in the assessment of cancer risk.…”

Section: Discussionmentioning

confidence: 82%

Micronuclei in the exfoliated oral epithelial cell: a cross-sectional study in Peruvian artisanal miners

Pizarro-Rojas,

Rabanal-Sanchez,

Soncco-Llulluy

et al. 2024

Int J Occup Saf Health

View full text Add to dashboard Cite

Introduction: Artisanal mining in Peru is an activity that generates a risk of exposure to different compounds, among which mercury and particulate matter stand out. The use of laboratory indicators to assess genotoxicity induced by work activity is a priority need. Our objective was to determine the micronucleus count in buccal cells of artisanal miners in Peru during 2020. Methods: We designed a cross-sectional study where 80 artisanal miners were evaluated who underwent scraping in the inner area of the cheek to obtain epithelial cells that were stained with Feulgen's staining, and micronuclei and nuclear alterations were identified on a count of 2000 cells. Results: We found that the mean micronucleus count was 27.5±8.0 (CI95: 25.7 – 29.3, min. 15, max. 48). For the other nuclear alterations such as nucleoplasmic bridges, budding, and binucleation, only up to one alteration was evidenced for each total count. The number of years of work (p=0.004) and age (p<0.001) were the only variables associated with the micronucleus count. Conclusion: The nuclear alterations in buccal cells of artisanal miners exposed to particulate material were micronuclei, nucleoplasmic bridges, budding, and binucleations, the most frequent being the presence of micronuclei, with a mean value of 27.5 micronuclei per 2000 cells counted.

show abstract

Section: Discussionmentioning

confidence: 82%

Micronuclei in the exfoliated oral epithelial cell: a cross-sectional study in Peruvian artisanal miners

Pizarro-Rojas,

Rabanal-Sanchez,

Soncco-Llulluy

et al. 2024

Int J Occup Saf Health

View full text Add to dashboard Cite

show abstract

Pathology of Oral Submucous Fibrosis

Ranganathan

Kavitha

2023

Textbooks in Contemporary Dentistry

View full text Add to dashboard Cite

Exploring Possible Diagnostic Precancerous Biomarkers for Oral Submucous Fibrosis: A Narrative Review

You,

Chen,

Hsieh

et al. 2023

Cancers

View full text Add to dashboard Cite

Oral submucous fibrosis (OSF) stands as a progressive oral ailment, designated as a potentially malignant disorder. OSF has gained widespread recognition as a significant precursor to malignant transformation. In the pursuit of dependable, straightforward, and non-invasive diagnostic measures for the early detection of oral malignant progression, research has delved into potential diagnostic biomarkers of OSF. This comprehensive review delves into current investigations that explore the correlation between various biomarkers and OSF. The molecular biomarkers of OSF are categorized based on cytology and sampling methods. Moreover, this review encompasses pertinent studies detailing how these biomarkers are acquired and processed. Within this scope, we scrutinize four potential biomarkers that hold the promise of facilitating the development of diagnostic tools for detecting early-stage OSF.

show abstract

Comparative evaluation of micronuclei in exfoliated oral epithelial cells in potentially malignant disorders and malignant lesions using special stains

Cited by 5 publications

References 8 publications

Micronuclei in the exfoliated oral epithelial cell: a cross-sectional study in Peruvian artisanal miners

Micronuclei in the exfoliated oral epithelial cell: a cross-sectional study in Peruvian artisanal miners

Pathology of Oral Submucous Fibrosis

Exploring Possible Diagnostic Precancerous Biomarkers for Oral Submucous Fibrosis: A Narrative Review

Contact Info

Product

Resources

About