Comparative Evaluation of Nimesulide-Loaded Nanoparticles for Anticancer Activity Against Breast Cancer Cells

Abstract

Recent clinical and epidemiological researches have declared that non-steroidal anti-inflammatory agents may display as antineoplastic agents and indicate pro-apoptotic and antiproliferative effects on cancer cells. The major purpose of this research was to develop a novel poly(ethyleneglycol)-block-poly(ε-caprolactone) (PEG-b-PCL) nano-sized particles encapsulated with nimesulide (NMS), a selective COX-2 inhibitor, and to evaluate its anticancer activity against MCF-7 breast cancer cells. NMS-encapsulated PEG-b-PCL nanoparticles were fabricated using three different production techniques: (i) by emulsion-solvent evaporation using a high shear homogenizer, (ii) by emulsion-solvent evaporation using an ultrasonicator, and (iii) by nanoprecipitation. Nanoparticles were evaluated with respect to the entrapment efficiency, size characteristics, drug release rates, thermal behavior, cell viability assays, and apoptosis. The resulting nanoparticles were found to be spherical shapes with negative surface charges. The average diameter of all nanoparticles ranged between 148.5 and 307.2 nm. In vitro release profiles showed that all nanoparticles exhibited a biphasic release pattern. NMS-loaded PEG-b-PCL nanoparticles demonstrated significant anticancer activity against MCF-7 breast cancer cells in a dose-dependent manner, and the effects of nanoparticles on cell proliferation were significantly affected by the preparation techniques. The nanoparticles developed in this work displayed higher potential for the NMS delivery against breast cancer treatment for the future.

Albumin binding and anticancer effect of magnesium oxide nanoparticles

“…Also, NPs can be used as drugs to induce antiproliferative activity against cancerous cells. 4,5 They can mediate reactive oxygen species (ROS) formation in the cancerous cells and switch on apoptotic, necrotic, and autophagic pathways. 6 Indeed, NPs due to their high accessible area are prone to interact with cell and organelle membranes leading to membrane leakage and inducing cancer cell ablation.…”

Formulation of nimesulide-loaded polylactide/poly(lactic-co-glycolic acid) nanoparticles and the evaluation of release kinetics

“…Its pain-relieving effect starts 15 min after oral application and it is used in the case of many pain symptoms [9]. NIM has already been the subject of research from the emulsions and solid nanostructures field of science [10][11][12][13][14][15][16][17][18][19]. Nimesulide-loaded nanoparticles have been formulated in two types of microemulsion: Epikuron 170 (E170)/isopropyl myristate/water/n-butanol (or isopropanol) [10].…”

“…Moreover, these systems showed a good loading capacity and drug release profile, and an in vitro antitumor activity comparable to a non-loaded one [15]. The novel poly(ethyleneglycol)-block-poly(ε-caprolactone) ( PEG-b-PCL) nano-sized particles encapsulated with NIM, a selective COX-2 inhibitor, were prepared to evaluate its anticancer activity against MCF-7 breast cancer cells [16]. These NIM-encapsulated PEG-b-PCL nanoparticles were fabricated using three different production techniques: (i) by emulsion-solvent evaporation using a high shear homogenizer, (ii) by emulsion-solvent evaporation using an ultrasonicator, and (iii) by nanoprecipitation [16].…”

“…The novel poly(ethyleneglycol)-block-poly(ε-caprolactone) ( PEG-b-PCL) nano-sized particles encapsulated with NIM, a selective COX-2 inhibitor, were prepared to evaluate its anticancer activity against MCF-7 breast cancer cells [16]. These NIM-encapsulated PEG-b-PCL nanoparticles were fabricated using three different production techniques: (i) by emulsion-solvent evaporation using a high shear homogenizer, (ii) by emulsion-solvent evaporation using an ultrasonicator, and (iii) by nanoprecipitation [16]. The nanoparticles were found to be of spherical shape with the average diameter of all nanoparticles ranging between 148.5 and 307.2 nm.…”

Synthesis and Different Effects of Biotinylated PAMAM G3 Dendrimer Substituted with Nimesulide in Human Normal Fibroblasts and Squamous Carcinoma Cells

“…Proposed targeted therapy with N includes conjugates with various vehicles. Among them, significant activity was shown with poly(ethyleneglycol)-block-poly(ε-caprolactone) (PEG-b-PCL) particles against MCF-7 breast cancer cells [30]. Using hyaluronic acid as carrier, the inhibition of colorectal tumor growth and induced cell death via apoptosis was observed in vivo in nude mice, without symptoms of hepatotoxicity [31].…”