Comparative evaluation of nutritional compositions between transgenic rice harboring the CaMsrB2 gene and the conventional counterpart

Cho, Yong-Hwa; Puligundla, Pradeep; Oh, Sung‐Dug; Park, Hyang-Mi; Kim, Kyung Min; Lee, Si-Myung; Ryu, Tae-Hun; Lee, Young‐Tack

doi:10.1007/s10068-016-0007-9

Cited by 8 publications

(7 citation statements)

References 25 publications

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“…The levels of these anti-nutrients like phytic acid, tannins and trypsin inhibitor in the Bt chickpea event were comparable to their non-transgenic chickpea seeds (Table 1) and were also within the range reported for chickpea varieties (Jukanti et al, 2012). Similar studies were also reported in transgenic pigeon pea (Mishra et al, 2017), rice (Cho et al, 2016) confirming them to be safe.…”

Section: Anti-nutrients Content In Bt Chickpea Seedssupporting

confidence: 79%

“…A comparative analysis is mostly adopted to evaluate that the transgenic and their non-transgenic counterparts are nutritionally equivalent. Several studies indicated that GM crops are considered safe if their nutritional composition is similar or data are within the range reported for their conventional counterparts (Mishra et al, 2017;Cho et al, 2016;Gayen et al, 2013;Junhua et al, 2005;Oberdoerfer et al, 2005;Wang et al, 2012). Therefore, we assessed the key composition determining seed protein quality of chickpea and compared the data with the nontransgenic parent (Jukanti et al, 2012), USDA, (2018).…”

Section: Resultsmentioning

confidence: 99%

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Assessment of Seed Protein Quality of A Transgenic Chickpea Event Expressing Cry2Aa Protein

Sarmah

Gupta

Acharjee

2020

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Background: Evaluation of the nutritional composition of genetically modified (GM) crops is mandatory for their deregulation. Chickpea is known for its high-quality protein and demonstrating that the seed protein quality of transgenic chickpea remains unaltered is important for its acceptance. Amino acid content, seed storage protein profile and the digestibility of chickpea protein are important determinants of seed protein quality. Thus, in the present study, we assessed the effect of Bt (Cry2Aa) gene expression on the Bt chickpea seed protein quality. Methods: We assessed the amino acid profile, in vitro protein digestibility and factors affecting protein digestibility like trypsin inhibitor, tannins and phytic acid contents of the transgenic Bt chickpea expressing a codon modified Cry2Aa gene and its non-transgenic counterpart. Furthermore, the seed storage proteins were also fractionated and separated on SDS-PAGE followed by mass spectroscopy of the major peptides. Result: Amino acid profile and factors affecting protein digestibility revealed no significant variations between transgenic and non-transgenic chickpeas. Seed storage protein profile confirmed the presence of legumin, vicilin and albumin. No potential change in the digestibility pattern of seed proteins was revealed. Our findings suggest no potential unintended changes in chickpea seed protein quality due to the expression of Cry2Aa gene.

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Section: Anti-nutrients Content In Bt Chickpea Seedssupporting

confidence: 79%

Section: Resultsmentioning

confidence: 99%

Assessment of Seed Protein Quality of A Transgenic Chickpea Event Expressing Cry2Aa Protein

Sarmah

Gupta

Acharjee

2020

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“…Similarly, AcoDREB16 and its homolog in rice (LOC_Os10g22600) are both highly expressed in roots. OsDREB2A, when overexpressed in rice, enhances salt stress tolerance (Mallikarjuna et al, 2011), without changing its total nutritional composition (Cornwell, 2014;Cho et al, 2016). Our analysis suggested that overexpression of some AcoDREBs in pineapple could help to develop new pineapple varieties with abiotic stress tolerance.…”

Section: Discussionmentioning

confidence: 82%

Identification and expression analysis of the DREB transcription factor family in pineapple (Ananas comosus (L.) Merr.)

Chai

Cheng

Yan

et al. 2020

PeerJ

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Background Dehydration responsive element-binding (DREB) transcription factors play a crucial role in plant growth, development and stress responses. Although DREB genes have been characterized in many plant species, genome-wide identification of the DREB gene family has not yet been reported in pineapple (Ananas comosus (L.) Merr.). Results Using comprehensive genome-wide screening, we identified 20 AcoDREB genes on 14 chromosomes. These were categorized into five subgroups. AcoDREBs within a group had similar gene structures and domain compositions. Using gene structure analysis, we showed that most AcoDREB genes (75%) lacked introns, and that the promoter regions of all 20 AcoDREB genes had at least one stress response-related cis-element. We identified four genes with high expression levels and six genes with low expression levels in all analyzed tissues. We detected expression changes under abiotic stress for eight selected AcoDREB genes. Conclusions This report presents the first genome-wide analysis of the DREB transcription factor family in pineapple. Our results provide preliminary data for future functional analysis of AcoDREB genes in pineapple, and useful information for developing new pineapple varieties with key agronomic traits such as stress tolerance.

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“…Mycotoxins, such as gibberellin and fumonisins, produced by F. f ujikuroi, not only affected the quality of rice but also did great harm to human and animal health. 5,6 Due to the production of gibberellin, the infected seedlings were slenderer than healthy seedlings, and the leaf sheaths were elongated abnormally. 7 Fungicide treatment of infected seeds is one of the most effective measures to control RBD.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Determination of Sensitivity of Fusarium fujikuroi to Fungicide Azoxystrobin and Investigation of Resistance Mechanism

Song

Chen

Sun

et al. 2022

J. Agric. Food Chem.

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Rice bakanae disease (RBD) caused by Fusarium fujikuroi is a widespread and destructive disease of rice. It is urgent to introduce a new class of fungicide to manage the fungicidal resistance problem and effectively control the disease. Azoxystrobin (AZO) is an active fungicide with a broad antifungal spectrum, while its activity against F. fujikuroi is not well investigated. In this study, the baseline sensitivity of F. fujikuroi to AZO was established by testing the sensitivity of 100 isolates, collected from Anhui Province of China. The mechanism of resistance to AZO was also investigated. AZO exhibited a strong activity against the 100 F. fujikuroi isolates with EC50 values of 0.822 ± 0.285 and 0.762 ± 0.283 μg/mL for mycelial growth and conidial germination, respectively, and both of the baseline sensitivity curves were validated as unimodal curves. To investigate the resistance mechanism, six mutants with resistance factor (RF) values >50 were generated from wild-type sensitive strains through UV mutagenesis, and sequence analysis showed that mutation G143A in cyt b conferred the resistance to AZO. Mycelial growth, conidia production, pathogenicity, and ATP production were decreased in all six resistant mutants as compared to the parental strains, indicating the fitness penalties in this phenotype of resistance mutation. In addition, the cross-resistance assay showed that there was no cross-resistance between AZO and carbendazim, prochloraz, phenamacril, or pydiflumetofen. AZO can be an efficient candidate to control RBD in China with moderate to low fungal resistance risk, but continuous resistance monitoring should be performed during the application of this fungicide.

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Comparative evaluation of nutritional compositions between transgenic rice harboring the CaMsrB2 gene and the conventional counterpart

Cited by 8 publications

References 25 publications

Assessment of Seed Protein Quality of A Transgenic Chickpea Event Expressing Cry2Aa Protein

Assessment of Seed Protein Quality of A Transgenic Chickpea Event Expressing Cry2Aa Protein

Identification and expression analysis of the DREB transcription factor family in pineapple (Ananas comosus (L.) Merr.)

In Vitro Determination of Sensitivity of Fusarium fujikuroi to Fungicide Azoxystrobin and Investigation of Resistance Mechanism

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