Comparative evaluation of periodontal ligament cell viability of permanent teeth in five different storage media followed by simulated avulsion injury

Abstract

Aim: To evaluate and compare the viability of periodontal ligament cells of avulsed teeth in five different storage media followed by simulated avulsion injury. Settings and Design: Seventy-five premolars extracted for orthodontic therapeutic purposes were randomly and equally ivided into five groups based on storage media used [A: HBSS (control), B: Milk (experimental), C: Aloe Vera (experimental), D: Egg white (experimental), E: Coconut water (experimental)].Methods and Material: Following extractions, the teeth were placed in one of the five different storage media for 60 minutes, following which the scrapings of the PDL were collected in Falcon tubes which already contained collagenase enzyme in 2.5 mL of Phosphate buffered saline. The tubes were subsequently incubated and centrifuged. Then acquired PDL cells were stained with Trypan Blue dye and were counted under an optical microscope. Statistical Analysis Used: Results were subjected to statistical analysis using ANOVA test and Post Hoc Tukey test. p-value < 0.05 is considered to be significant. Results: HBSS showed the highest percentage of viable cells (80.14%), followed by Egg white (74.01%) and Aloe Vera (73.68%). Milk and Coconut water showed the least percent of viable cells 63.20%, 63.58% respectively.

Assessment of Periodontal Ligament Fibroblast Viability in Dymatize® Elite Casein as a Transport Medium for Avulsed Teeth at Different Time Intervals (An in - vitro study)

“…In this study, the experimental groups were (30 teeth) for each:  HBSS group: In this group, teeth were immersed in Hank`s balanced salt solution directly after extraction and stored for different time intervals (1 hr., 4 hr., and 8 hr. ), 10 teeth for each time interval (Patel et al, 2022).  Dymatize® Elite Casein (micellar casein) group: In this group, teeth were immersed in this solution as a storage medium for time intervals of 1hr.,4hr., and 8 hr., 10 teeth for each time interval.…”