Comparative evaluation of platelet counts in two hematology analyzers and potential effects on prophylactic platelet transfusion decisions

Hummel, Kornelia; Sachse, Michaela; Hoffmann, J. J. M. L.; Dun, Ludi P.J.M. van

doi:10.1111/trf.14886

Cited by 17 publications

(17 citation statements)

References 25 publications

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“…However, if the RBC or platelet size histograms are abnormal or if the platelet count is below a preset limit, the PLT‐F method is performed as a reflex test to improve accuracy by clearly distinguishing platelets from other blood cells 11 . The performance of XN‐PLT‐F focusing on accurate detection of low platelet counts was evaluated in several studies 1,11‐13 . These studies reported that XN‐PLT‐F was a reliable method for low platelet counting and was preferred method to make appropriate decisions regarding platelet transfusions in thrombocytopenic patients, which was consistent with our result.…”

Section: Discussionsupporting

confidence: 86%

Performance evaluation of platelet counting of Abbott Alinity hq and Sysmex XN‐9000 automated hematology analyzer compared with international reference method

Kim

Bang

Cho

et al. 2020

Int J Lab Hematology

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Introduction Accurate platelet counting is essential for risk assessment of bleeding and thrombosis. Abbott Alinity hq hematology analyzer was recently introduced, and its performance in platelet counting has yet to be evaluated comprehensively. In this study, we evaluated the performance of the optical platelet counting of Abbott Alinity hq (Alinity‐PLT) and the impedance and fluorescent platelet counting of Sysmex XN‐9000 (XN‐PLT‐I and XN‐PLT‐F) compared with the international reference method. Methods Blood samples were analyzed via Alinity hq and XN‐9000 with PLT‐F channel. Immuno‐platelet (ImmnoPLT) reference method was performed with CD41/CD61 antibodies using FACSLyricTM flow cytometer (BD). Precision was determined using 10 replicates in a single run, and the platelet counts of Alinity‐PLT, XN‐PLT‐I, XN‐PLT‐F, and ImmnoPLT were compared. Results At a platelet count of 13 × 109/L, the CVs of Alinity‐PLT, XN‐PLT‐I, and XN‐PLT‐F were 4.2%, 6.7%, and 4.3%, respectively, and at a platelet count of 44 × 109/L, all showed a CV of less than 3%. For the total 210 samples, all three methods showed a very strong correlation with ImmunoPLT (r > 0.99). For platelet levels below 20 × 109/L, XN‐PLT‐F showed the strongest correlation with ImmunoPLT (r = 0.975), and for platelet levels of 20‐100 × 109/L, Alinity‐PLT and XN‐PLT‐I were comparable to ImmunoPLT. For platelet levels of 100‐450 × 109/L, XN‐PLT‐I was the most comparable to ImmunoPLT, and for platelet levels above 450 × 109/L, Alinity‐PLT was comparable to ImmunoPLT. Conclusions All three methods were highly correlated with ImmunoPLT, and each method had different performance advantages according to the platelet levels.

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Section: Discussionsupporting

confidence: 86%

Performance evaluation of platelet counting of Abbott Alinity hq and Sysmex XN‐9000 automated hematology analyzer compared with international reference method

Kim

Bang

Cho

et al. 2020

Int J Lab Hematology

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“…The prophylactic transfusion threshold in low-risk patients is generally considered to be 10 or 20 × 10 9 /L, 1-3 making the accuracy of the PLT count by automated hematology analyzers a fundamental issue for physicians to provide effective care. [4][5][6] Despite significant advancements in PLT enumeration accuracy and precision, 7 approximately two-third of hematology analyzers have been shown to overestimate the PLT concentration in thrombocytopenic…”

mentioning

confidence: 99%

Alinity hq platelet results are equivalent with the international reference method in thrombocytopenic samples

Lifson

Wakui

Arai

et al. 2021

Int J Lab Hematology

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Providing an accurate platelet (PLT) count is essential for properly managing thrombocytopenic patients at risk of bleeding and for determining the need for PLT transfusion. The prophylactic transfusion threshold in low-risk patients is generally considered to be 10 or 20 × 10 9 /L, 1-3 making the accuracy of the PLT count by automated hematology analyzers a fundamental issue for physicians to provide effective care. [4][5][6] Despite significant advancements in PLT enumeration accuracy and precision, 7 approximately two-third of hematology analyzers have been shown to overestimate the PLT concentration in thrombocytopenic

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“…red cell "ghosts") in the scatterplot of cell size (forward light scatter) vs. RNA content (fluorescence intensity). The XN-series also shows improved precision of platelet counts below 50 × 10 9 /L and better correlation (r 2 ≥ 0.80) with the flow cytometric reference method for platelet enumeration due to a 5 times larger sample volume analyzed [24,27,28,[30][31][32]. This progress in the XN-series may account for the moderate correlation of IPF between the XE-2100 and the XN-2000 (r 2 ≥ 0.60) [24,27] and potentially improves the utility of IPF especially in hematological patients with very low platelet counts and in clinical settings with interference of aberrant cell populations and debris (e.g.…”

Section: Automated Determination Of Reticulated Plateletsmentioning

confidence: 93%

Reticulated platelets – clinical application and future perspectives

Meintker

Krause

2020

Journal of Laboratory Medicine

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Reticulated platelets are immature platelets freshly released from the bone marrow into the circulation and contain vestigial amounts of ribonucleic acid. Thus, they can serve as an indicator for the activity of thrombopoiesis. Despite the current lack of a standardized reference method, two types of hematology analyzers have incorporated a fully automated measurement of reticulated platelets. The “immature platelet fraction” (IPF; Sysmex XE-/XN-series) has some clinical utility in the differential diagnosis of thrombocytopenia. This is less clear for “reticulated platelets” (retPLT; Abbott CELL-DYN Sapphire/Alinity HQ). The usefulness of these parameters in the prediction of platelet recovery after chemotherapy or stem cell transplantation and as a decision aid for platelet transfusions has not been unequivocally confirmed. Recent findings have shown an association of reticulated platelets with an adverse risk in patients with coronary artery disease and stroke as well as resistance to anti-platelet therapy. Furthermore, a role of reticulated platelets for the prediction of sepsis was indicated. However, validation in larger prospective trials is necessary to establish the clinical benefit of reticulated platelets in these conditions. This review gives an overview of the available analytical methods and summarizes the current knowledge regarding the clinical application of reticulated platelets.

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Comparative evaluation of platelet counts in two hematology analyzers and potential effects on prophylactic platelet transfusion decisions

Cited by 17 publications

References 25 publications

Performance evaluation of platelet counting of Abbott Alinity hq and Sysmex XN‐9000 automated hematology analyzer compared with international reference method

Performance evaluation of platelet counting of Abbott Alinity hq and Sysmex XN‐9000 automated hematology analyzer compared with international reference method

Alinity hq platelet results are equivalent with the international reference method in thrombocytopenic samples

Reticulated platelets – clinical application and future perspectives

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