Comparative Evaluation of Polyphenol Contents and Biological Activities of Five Cistus L. Species Native to Turkey

Abstract

In this study phytochemical compounds and antioxidant capacity, cytotoxic, antimicrobial and anti-biofilm activities of hydroethanolic extracts of five Cistus species (C. creticus L., C. laurifolius L., C. monspeliensis L., C. parviflorus Lam. and C. salviifolius L.) distributed in Turkey were investigated. (+)-catechin, epigallocatechin gallate, quercetin-3-O-rutinoside, quercetin-3-O-glucoside, kaempferol-3-O-glucoside, luteolin were detected in different amounts. Strongest antioxidant capacities were observed with C. creticus, and C. parvifolius (0.476 and 0.452, respectively). Minimum inhibitory concentrations (MIC) of the extracts were determined between 32 and 128 μg/mL against different bacteria and Candida strains. C. monspeliensis and C. laurifolius extracts were inhibited the biofilm production levels of three Gram-negative bacteria (E. coli, S. enterica, P. aeruginosa), two Gram-positive bacteria (S. aureus, B. subtilis) and three Candida strains (C. albicans, C. parapsilosis, C. krusei). C. creticus extract showed strongest cytotoxic activity against human breast adenocarcinoma (MCF-7) and prostate cell lines (PC-3) (IC 50 : 14.04 � 2.78 μg/mL and 34.04 � 2.74 μg/mL, respectively) among all plants tested.

Polyphenol Fingerprint, Biological Activities, and In Silico Studies of the Medicinal Plant Cistus parviflorus L. Extract

“…The analysis also revealed the presence of epigallocatechin gallate, luteolin, quercetin-3-glucoside, and catechin in the extract of C. parviflorus at concentrations of 5.78, 15.21, 0.84, and 5.35, respectively . The findings of the study by Onal et al demonstrated the plant’s biogenetic capacity to synthesize various flavonoids and demonstrated that C.…”

“…In addition, they have detected kaempferol-3-glucoside only in C. criticus . The analysis also revealed the presence of epigallocatechin gallate, luteolin, quercetin-3-glucoside, and catechin in the extract of C.…”

Efficacy of Two Moroccan Cistus Species Extracts against Acne Vulgaris: Phytochemical Profile, Antioxidant, Anti-Inflammatory and Antimicrobial Activities

“…Moreover, Álvarez-Martínez et al [ 35 ] investigated the activity of C. salviifolius against 100 S. aureus clinical isolates and referred MIC 50 values ranging between 50–80 μg/mL. The authors also documented that the Cistus extracts containing hydrolysable tannins and flavonoids such as myricetin and quercetin derivatives showed the higher activity against MRSA isolates.Among the Cistus extracts, C. laurifolius exhibited antibacterial efficacy against S. aureus and E. coli [ 36 ] and inhibitory activity against microbial biofilm production [ 37 ].…”

Staphylococcus aureus biofilm: Formulation, regulatory, and emerging natural products-derived therapeutics

“…The hydroethanolic extracts of numerous plants have been reported to significantly reduce the S. aureus biofilm formation, which could be explained by the presence of chlorogenic, quercetin, and rutin [ [111] , [112] , [113] ]. Likewise, due to rich and diverse phytochemical compositions, the methanolic extract of Capsicum annuum at 64 μg/mL showed an inhibition rate of 53.8 % against S. aureus biofilm, comparable to amoxicillin [ 114 ].…”