Comparative evaluation of remineralization potential of casein phosphopeptide- amorphous calcium fluoride phosphate and novamin on artificially demineralized human enamel: An In vitro study

Manoharan, Vidya; Kumar, R Krishna; Sivanraj, Arun Kumar; Arumugam, Selva Balaji

doi:10.4103/ccd.ccd_28_18

Cited by 26 publications

(34 citation statements)

References 15 publications

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“…The use of milk and milk products, having a protective effect against the development of dental caries, has been a novel concept in remineralization. The anticariogenic properties of milk is due to the presence of casein, calcium and phosphate, which are responsible for resistance to acid dissolution (5,6). One such, remineralizing agent which has been extensively studied and accepted is casein phosphopeptide amorphous calcium phosphate (CPP-ACP).…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of the remineralization potential of CPP–ACP with Fluoride, Tri-Calcium Phosphate and Nano Hydroxyapatite using SEM/EDX – An in vitro study

Thimmaiah¹,

Shetty

et al. 2019

J Clin Exp Dent

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BackgroundIn recent years, the non-invasive management of non cavitated caries lesions using remineralization systems to repair the enamel have received more attention from the scientific community. Aim: To quantitatively evaluate the remineralization potential of Casein phosphopeptide-amorphous calcium phosphate-fluoride(CPP-ACPF), Tri-calcium phosphate(TCP) & Nano-hydroxyapatite(nHAP) using Scanning Electron Microscopy(SEM) and Energy dispersive X-ray Analysis(EDX).Material and Methods40 enamel specimens were prepared, and immersed in demineralising solution at a pH of 4.4 for 96 hours at 37°C, to induce artificial carious lesions. Remineralization was carried out for a period of 30 days using CPP-ACPF, TCP, nHAP. The specimens were evaluated for calcium and phosphorus content using SEM-EDX.ResultsThe Ca/P mass % after remineralization was significantly higher with CPP-ACP-F and TCP-F followed by nHAP.ConclusionsCPP-ACP-F and TCP can promote significant remineralization of incipient carious lesions. These are excellent delivery vehicles available in a slow release amorphous form to localize calcium, phosphate and fluoride at the tooth surface. Key words:Remineralization, in vitro; CPP-ACP fluoride, Nano-hydroxyapatite, Tri-calcium phosphate, SEM/ EDX.

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Section: Introductionmentioning

confidence: 99%

Comparative analysis of the remineralization potential of CPP–ACP with Fluoride, Tri-Calcium Phosphate and Nano Hydroxyapatite using SEM/EDX – An in vitro study

Thimmaiah¹,

Shetty

et al. 2019

J Clin Exp Dent

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“…As the remineralization of enamel requires the availability of Ca and P in the oral environment, materials providing essential elements for remineralization are needed. Among these, CPP-ACP (Recaldent™) emerged as a novel material that prevented demineralization, and showed enhanced remineralization of initial enamel lesions in vitro and in vivo studies [18,[20][21][22][23][24][25]. Additionally, the concomitant use of CPP-ACP (Recaldent™) JOURNAL OF ORAL & MAXILLOFACIAL RESEARCH Zeitouny et al…”

Section: Discussionmentioning

confidence: 99%

The Efficacy of Soprolife® in Detecting in Vitro Remineralization of Early Caries Lesions

Zeitouny¹,

Cuisinier²,

Tassery³

et al. 2020

JOMR

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Objectives This randomized controlled in vitro 4-arm trial study aimed to evaluate the efficacy of SoproLife ® in detecting and quantifying in vitro remineralization with early caries lesions. Material and Methods Sixty human teeth were randomly assigned into four equal groups. Groups 1 and 2 were prophylactically cleaned; groups 3 and 4 were not. Group 1 received treatment with MI Varnish ® and Recaldent™ for 30 days. Group 2 was treated similarly, but without MI Varnish ® . Group 3 was treated as 1 and Group 4 as 2. Mineral composition was obtained using scanning electron microscopy with energy dispersive X-ray analysis SoproLife ® camera images on the occlusal surfaces were analysed for grey value distribution and difference in mean intensity values (DI). Paired t-test and Mann-Whitney-U test were used for intragroup comparison between baseline and T1. Kruskal-Wallis followed by Mann-Whitney-U tests were used for inter-group comparisons at T1. Results All groups exhibited a significant increase in calcium content and calcium-to-phosphorus ratio (P < 0.05), except Group 4 (Group 1 showed the greatest increase, then Groups 3 and 2). Grey intensity values decreased in all groups (P < 0.05). Group 1 showed the greatest change in DI (16.82 [SD 12.07]), followed by Group 3 (12.46 [SD 9.41]), 2 (10.45 [SD 7.76]), and 4 (6.46 [SD 6.21]). The difference in DI was different between the compared groups (P = 0.038); Groups 1 and 3 exhibited a greater DI compared with 4 (P < 0.01). Conclusions Within the limitations of this study, SoproLife ® is effective for early detection and for longitudinally monitoring the remineralization after Recaldent™ therapy.

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“…In the oral environment, CPP-ACP binds to biofilms, plaque, bacteria, hydroxyapatite, and soft tissue, buffers the free Ca and phosphate ion activities and increases the degree of saturation by Ca and P with respect to hydroxyapatite. Thus CPP-ACP localizes bioavailable ions and subsequently ACP on the tooth surface [15]. Only, a very limited number of studies had evaluated factors modifying the remineralization potential of CPP-ACP quantitatively, especially the Ca and P content, and Ca/P of enamel [18,19].…”

Section: Discussionmentioning

confidence: 99%

“…CPP-ACP adheres intraorally to plaque pellicle, hydroxyapatite as well as soft tissues, and was shown to buffer the free calcium and phosphate ion activities, maintain a state of super-saturation with respect to tooth enamel, prevent enamel demineralization, and promote remineralization [14]. A series of studies and systematic reviews [15,16] have reported an anti-caries effect of CPP-ACP. CCP-ACP with 0.2% sodium fluoride (Recaldent™ GC Corporation; Tokyo, Japan) is delivered in many forms including tooth mousse, toothpastes, mouth rinses, chewing gums, sports drinks, as well as in dental varnishes [11,17].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Influence of Prophylaxis Cleaning on Enamel Remineralization with Casein Phosphopeptide-Amorphous Calcium Phosphate

Zeitouny

Fayyad-Kazan

Hervé

et al. 2019

JOMR

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Objectives: This randomized controlled in vitro 4-arm trial study aimed to study the remineralization potential of Recaldent™ and assess the effects of prophylaxis cleaning and MI Varnish ® on enhancing this remineralization potential. Material and Methods: Sixty human teeth were randomly assigned into equal samples (A/B). Sample A was prophylactically cleaned, randomly divided into equal samples (1/2). Sample A1 received treatment with MI Varnish ® and Recaldent™ for 30 days. Sample A2 was treated similarly but without MI Varnish ® . Sample B did not receive prophylaxis cleaning and was divided into equal samples (1/2). Sample B1 was treated as A1 and sample B2 as A2. The teeth were examined for mineral composition at baseline, after the interventions (T1), and after prophylaxis cleaning (T2). Study outcomes were mineral content (% weight of carbon [C], phosphorus [P], calcium [Ca], oxygen [O], chlorine [Cl], sodium [Na] and silicon [Si]) and calcium-phosphorus ratio (Ca/P). Results: All groups had similar mineral composition at baseline. At T1, sample B2 exhibited least P, Ca and Ca/P content. Samples A1 and B1 showed higher content of P and Ca, compared to B2 (A1 only exhibited higher Ca/P). Sample A2 exhibited lowest Cl and Na content. At T2, sample A1 exhibited lowest C, P, O and Si content (highest Ca/P). Sample A2 showed least Ca/P, and highest Na content. Conclusions: Teeth treated by Recaldent™ proceeded by prophylaxis cleaning or MI Varnish ® showed remineralization, especially when receiving both interventions. This superior effect persisted even after a second cleaning. Further trials are necessary to provide conclusive evidence in humans.

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Comparative evaluation of remineralization potential of casein phosphopeptide- amorphous calcium fluoride phosphate and novamin on artificially demineralized human enamel: An In vitro study

Cited by 26 publications

References 15 publications

Comparative analysis of the remineralization potential of CPP–ACP with Fluoride, Tri-Calcium Phosphate and Nano Hydroxyapatite using SEM/EDX – An in vitro study

Comparative analysis of the remineralization potential of CPP–ACP with Fluoride, Tri-Calcium Phosphate and Nano Hydroxyapatite using SEM/EDX – An in vitro study

The Efficacy of Soprolife® in Detecting in Vitro Remineralization of Early Caries Lesions

In Vitro Influence of Prophylaxis Cleaning on Enamel Remineralization with Casein Phosphopeptide-Amorphous Calcium Phosphate

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