Comparative Evaluation of RUT, PCR and ELISA Tests for Detection of Infection with Cytotoxigenic H. pylori

Jalalypour, Farzaneh; Farajnia, Safar; Somi, Mohammad Hossein; Hojabri, Zoya; Yousefzadeh, Rana; Saeedi, Nazli

doi:10.15171/apb.2016.036

Cited by 15 publications

(13 citation statements)

References 34 publications

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“…The current results regarding serum H. pylori IgG, revealed a sensitivity of 75% and specificity of 79%, but Khalilpour et al found the sensitivity and specificity of serum IgG up to 100%, which was substantially more than us ( 20 ). An investigation in Iran, showed high sensitivity (90.2%) and low specificity (61.1%) of serum H. pylori IgG, which were incompatible with our results ( 21 ). Rahman et al reported sensitivity and specificity of serum IgG against H. pylori about 96.7% and 42.8% respectively, that the sensitivity was more than our findings and the specificity was lesser than our results ( 22 ).…”

Section: Discussioncontrasting

confidence: 99%

Comparison of the serum and salivary antibodies to detect gastric Helicobacter pylori infection in Kashan (Iran)

et al. 2017

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Background and aimHelicobacter pylori (H. pylori) is an important and common contagious human pathogen which may cause peptic ulcer and also gastric cancer. The definite diagnosis of it is made through invasive tests. Recently, non-invasive tests including serologic tests of serum and saliva have been conducted for diagnosis of H. pylori infection. In this research, the diagnostic values of serum and salivary serology were compared together to use salivary anti-H. pylori test as an alternative method in the future.MethodsDuring this prospective case-control study on patients who were candidates for endoscopy and gastric biopsy from March 2015 to April 2016 in Shahid Beheshti hospital, Kashan, Iran, serum and salivary samples were obtained for measurement of Immunoglobulin G (IgG) antibody levels against H. pylori by enzyme-linked immunosorbent assay (ELISA). Histopathology was the gold standard test. Statistical analysis was performed by SPSS software version 16. Statistical tests included Kolmogorov-Smirnov, independent-samples t-test, Chi-square, Mann-Whitney U, Kruskal-Wallis, McNemar and correlation.ResultsOf 123 patients, sixty-one patients (49.6%) were H. pylori-positive according to histology. The median levels of anti-H. pylori antibodies in serum (p<0.001) and saliva (p<0.001) of H. pylori-positive cases were significantly higher than H. pylori-negative cases. Sensitivity, specificity, positive likelihood ratio, negative likelihood ratio and accuracy of serologic tests in serum were 75%, 79%, 3.5, 0.3, 77% and for saliva were 85%, 82%, 4.7, 0.18, 84% respectively.ConclusionDiagnostic values of salivary ELISA are comparable to serum ELISA and can be used as an alternative modality for diagnosis of H. pylori infection.

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Section: Discussioncontrasting

confidence: 99%

Comparison of the serum and salivary antibodies to detect gastric Helicobacter pylori infection in Kashan (Iran)

et al. 2017

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“…Several studies considered that one invasive test could be used for diagnosis of H. pylori infection and confirmation of H. pylori eradication after treatment [ 26 , 27 ]. On the other hand, other studies considered that the combination of two positive invasive tests is required for diagnosis of H. pylori infection [ 28 ]. The present study used the combination of RUT and PCR for diagnosis of H. pylori infection.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Helicobacter pylori cagA and iceA Genes and Their Association with Gastrointestinal Diseases

Abutaleb

Abdelattef

Abdel-Hady

et al. 2018

International Journal of Microbiology

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H. pylori infection causes peptic ulcer, chronic gastritis, mucosa-associated lymphoid tissue lymphoma, and gastric carcinoma. It has several virulence factors such as cytotoxin-associated gene A(cagA) and the induced by contact with epithelium antigen (iceA). We aimed to explore the relationship between cagA and iceA of H. pylori and gastrointestinal diseases. One hundred and eighteen patients who attended Gastrointestinal Endoscopy Unit at Zagazig University Hospitals, Egypt, were included in this study. Two gastric biopsies were collected and evaluated by rapid urease test (RUT) and PCR. cagA and iceA genes were amplified by PCR. We found that 54 patients (45.76%) were positive by both RUT and PCR. cagA and iceA genes were present in 57.4% and 46.29% of the studied patients, respectively. cagA was the most prevalent gene in gastritis (33.3%) and peptic ulcer (68.7%). iceA1/iceA2 positive genes were the most prevalent in gastric cancer (75%). iceA1 gene was present in 38.7% of cagA positive cases, but iceA2 gene was present in 45.2% of cagA positive cases. iceA1/iceA2 positive genes were present in 29% of cagA positive cases. In conclusion, cagA and iceA genes could be used as markers for severe gastrointestinal diseases. iceA gene was strongly related to cagA gene.

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“…Recently, emphasis has been placed on the use of non-invasive diagnostic methods for the detection of H. pylori in pediatric population [ 23 , 24 ]; however, their use is not yet standardized in routine clinical practice. The PCR is among the most promising techniques for being faster, more accurate and specificity [ 16 , 25 ]. This method can detect H. pylori regardless of their viability with high sensitivity and specificity arriving to 95% [ 26 ].…”

Section: Main Textmentioning

confidence: 99%

“…This method can detect H. pylori regardless of their viability with high sensitivity and specificity arriving to 95% [ 26 ]. PCR presents greater specificity than the rapid urease test (RUT) [ 25 ]. In addition, PCR has the advantage of identifying H. pylori with a limited quantity of bacterial load allowing to recognize the infection when other tests give negative results [ 27 ].…”

Section: Main Textmentioning

confidence: 99%

Highly clarithromycin-resistant Helicobacter pylori infection in asymptomatic children from a rural community of Cajamarca-Peru

et al. 2018

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ObjectiveThe objective of this study was to determine the prevalence of clarithromycin-resistant Helicobacter pylori in asymptomatic children in a rural community of Cajamarca (northern Peru).ResultsHelicobacter pylori was detected in 17.2% (49/285) of the samples. Unboiled water consumption the most frequent associated factor in patients with positive PCR for H. pylori infection (93.9%). Clarithromycin resistant mutations were found in 79.6% (39/49) of the positive samples for H. pylori. The most frequent mutation was A2142G (46.9%), followed by the double-mutation A2142G–A2143G (28.6%).

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Comparative Evaluation of RUT, PCR and ELISA Tests for Detection of Infection with Cytotoxigenic H. pylori

Cited by 15 publications

References 34 publications

Comparison of the serum and salivary antibodies to detect gastric Helicobacter pylori infection in Kashan (Iran)

Comparison of the serum and salivary antibodies to detect gastric Helicobacter pylori infection in Kashan (Iran)

Prevalence of Helicobacter pylori cagA and iceA Genes and Their Association with Gastrointestinal Diseases

Highly clarithromycin-resistant Helicobacter pylori infection in asymptomatic children from a rural community of Cajamarca-Peru

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