Comparative evaluation of safety and efficacy of a live Salmonella gallinarum vaccine candidate secreting an adjuvant protein with SG9R in chickens

Nandre, Rahul; Lee, John Hwa

doi:10.1016/j.vetimm.2014.08.014

Cited by 10 publications

(9 citation statements)

References 43 publications

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“…JOL1355 is an attenuated mutant S. Gallinarum strain (Δlon ΔcpxR Δasd) carrying a low copy number plasmid (p15A ori) with eltB encoding the LTB (Nandre & Lee, 2014). Prior to the infection of chicken DCs, bacteria were grown overnight in Luria-Bertani (LB) broth (Becton, Dickinson and Company, Sparks, MD, USA).…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

“…In our efforts to develop a safe and efficacious FT vaccine candidate, we have constructed a SG lon/cpxR deletion mutant (JOL1355) carrying an eltB gene expression plasmid for the production of Escherichia coli heat-labile enterotoxin B subunit protein (LTB) (Nandre & Lee, 2014). It is well established that vaccines do not react directly with the immune system but must first be captured, processed, and bound to antigen-presenting cells such as macrophages and DCs (Steinman & Pope, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Interaction of a live attenuatedSalmonellaGallinarum vaccine candidate with chicken bone marrow-derived dendritic cells

2016

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Salmonella enterica serovar Gallinarum (SG) is a Gram-negative intracellular host-adapted pathogen that causes fowl typhoid. Attenuated strains of SG are proven and widely used vaccine candidates because of advantages like induction of strong humoral and cell-mediated immune responses. In the present study, we investigated the interaction of chicken bone marrow-derived dendritic cells (chBM-DCs) with an attenuated SG (JOL1355) strain that secretes a heat-labile enterotoxin B subunit protein previously shown to successfully vaccinate chickens. ChBM-DCs were isolated and cultured in the presence of recombinant chicken GM-CSF and IL-4 cytokines. The chBM-DCs were infected with JOL1355 at an multiplicity of infection of 10. JOL1355 was able to invade dendritic cells (DCs); however, the survival of JOL1355 in DCs decreased over time. At 24 h post infection, IL-6, IL-10 and IFN-γ transcript levels were significantly increased in JOL1355-infected DCs compared to non-stimulated DCs. Flow cytometry analysis showed an increased proportion of cells producing CD40, CD80, and MHC class II in the JOL1355-infected cultures compared to the non-stimulated control. In addition, JOL1355-stimulated chBM-DCs could induce significant expression of IL-2 in co-culture with autologous CD4+ T cells. Based on these results, we conclude that chBM-DCs are capable of internalizing the live attenuated SG vaccine candidate and the infected chBM-DCs show signs of maturation as evidenced by the upregulated expression of costimulatory molecules and cytokines.

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Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Interaction of a live attenuatedSalmonellaGallinarum vaccine candidate with chicken bone marrow-derived dendritic cells

2016

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“…Live attenuated vaccines are frequently used for effective protection against fowl typhoid. However, safety is a major concern when using live vaccines due to their residual virulence (Kwon and Cho, 2011) and long persistence in the internal organs of immunised chickens, which may cause adverse effects in immunised hosts and contaminate the environment via shedding of live attenuated strains through faeces (Nandre and Lee, 2014). Thus, we have directed efforts towards the development of genetically inactivated bacterial ghost-based vaccines.…”

Section: Discussionmentioning

confidence: 99%

“…Gross hepatic and splenic lesions were scored as 0, 1, 2 or 3, using a scoring system described previously (Chaudhari et al, 2012). Detection of the challenge strain in the liver and spleen tissues was carried out as described previously (Nandre and Lee, 2014).…”

Section: Protection Against Virulent Efficacy Challengementioning

confidence: 99%

Evaluation of Salmonella Gallinarum ghost formulated with Montanide™ ISA 70 VG adjuvant as a vaccine against fowl typhoid

Jawale

Somsanith

et al. 2015

Acta Veterinaria Hungarica

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Escherichia coli heat-labile enterotoxin B subunit (LTB) protein is a potent adjuvant. Salmonella Gallinarum ghosts carrying LTB (S. Gallinarum-LTB ghosts) were genetically constructed using a plasmid, pJHL187-LTB, designed for the co-expression of the LTB and E lysis proteins. This study evaluates the immunopotentiating effects of Montanide TM ISA 70 VG on S. Gallinarum-LTB ghost vaccination against fowl typhoid. Five-week-old layer chickens were injected intramuscularly with sterile PBS (non-immunised control, Group A), S. Gallinarum-LTB ghost (Group B) or S. Gallinarum-LTB ghost emulsified with Montanide TM ISA 70 VG adjuvant (Group C). Chickens from both Groups B and C showed significant induction of antigen-specific systemic IgG response compared to controls; in addition, Group C showed enhanced induction of systemic IgG response compared to Group B. We observed significant induction of antigen-specific lymphocyte proliferative response and increased mRNA levels of Th1 cytokines (IFN-γ and IL2) in both Groups B and C. Furthermore, in the challenge experiment with a virulent strain of S. Gallinarum, Group C showed higher survival rates compared with other groups. These results indicate that vaccination with the S. Gallinarum-LTB ghost in combination with Montanide TM ISA 70 VG may enhance the protective immunity against fowl typhoid.

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“…가금티푸스의 예방은 주로 백신 접종을 통해 이루어지며, 이 질병에 대한 연구도 S. gallinarum에 대한 다양한 백신 개발에 집중되고 있다 (Geng et al, 2014;Jawale et al, 2014;Nandre and Lee, 2014). 그러나 고병원성 병원균 등의 원인으로 백신의 효과가 완전하지 못하여 알로에 추출물 등의 투여 (Waihenya et al, 2002) …”

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Isolation and characterization of Bacillus amyloliquefaciens TK3 inhibiting causative bacteria of atrophic rhinitis and fowl typhoid

Jung

Song

2015

The Korean Journal of Microbiology

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For prevention of atrophic rhinitis of swine by Bordetella bronchiseptica and fowl typhoid by Salmonella gallinarum, bacterial strains showing antimicrobial activity against those pathogenic bacteria were isolated from various samples collected at animal farms. Among 372 bacterial isolates strain TK3 showed the highest antibacterial activity against both pathogens, and was identified as Bacillus amyloliquefaciens by 16S rRNA gene sequence analysis. B. amyloliquefaciens TK3 could inhibit growth of both pathogens by secretion of antibacterial compounds such as siderophore, rhamnolipid and antimicrobial peptide. Production radius of siderophore on Chrome azurol S agar plate by strain TK3 was 0.53 cm after 14 days of incubation, and concentration of siderophore in King's B medium was 1.06 mmol/ml. It also secreted 82.4 mg/L of rhamnolipid, and antimicrobial peptide that completely inhibited growth of both pathogens at concentration of 30 μl/ml in LB medium.

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Comparative evaluation of safety and efficacy of a live Salmonella gallinarum vaccine candidate secreting an adjuvant protein with SG9R in chickens

Cited by 10 publications

References 43 publications

Interaction of a live attenuatedSalmonellaGallinarum vaccine candidate with chicken bone marrow-derived dendritic cells

Interaction of a live attenuatedSalmonellaGallinarum vaccine candidate with chicken bone marrow-derived dendritic cells

Evaluation of Salmonella Gallinarum ghost formulated with Montanide™ ISA 70 VG adjuvant as a vaccine against fowl typhoid

Isolation and characterization of Bacillus amyloliquefaciens TK3 inhibiting causative bacteria of atrophic rhinitis and fowl typhoid

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