Comparative Evaluation of Soluble and Insoluble-Bound Phenolics and Antioxidant Activity of Two Chinese Mistletoes

Abstract

Mistletoes are used medicinally in order to treat various human illnesses. Few studies have reported on the phenolic content and antioxidant properties of Chinese mistletoes (CMs). In this work, the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities of soluble and insoluble-bound phenolic extracts from CMs hosted by Camellia assamica (Mast.) Chang (CMC) and Pyrus, i, f. (CMP) were compared. Phenolic compounds in CMC and CMP were identified and quantified using high-performance liquid chromatography (HPLC). The results indicated that the TPC of soluble phenolic extracts was higher than insoluble-bound phenolic counterparts in both CMC and CMP. In addition, the TPC of soluble, insoluble-bound and total phenolic fractions (9.91 ± 0.23, 4.59 ± 0.27 and 14.50 ± 0.35 μmol ferulic acid equivalents per gram (FAE/g) dry sample) extracted from CMP were higher than those extracted from CMC. The soluble phenolic extracts in CMP showed higher antioxidant activities than those in CMC. Eighteen phenolic compounds from soluble and insoluble-bound phenolic extracts from the CMs were identified and quantified by HPLC. This study indicates that CMC and CMP, especially the latter, could be sources of antioxidants in human health care.

Impact of Carbon Nanomaterials on the Antioxidant System of Tomato Seedlings

“…Phenolic compounds are a chemically diverse group of secondary metabolites that occur ubiquitously in plants [37]. They can be divided into several groups, including phenolic acids, flavonoids, stilbenes, and lignans, depending on their chemical structure [38]. They commonly act as chemical and physical barriers to protect plants against biotic and abiotic stress [39], provide protection against excess sunlight by absorbing high energy wavelengths [40], and they can also protect plants from various pests and diseases [41].…”

Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae

“…The compositions of bound phenolic components in the SDF and IDF were analyzed according to a published method (Li et al., 2018). In brief, after the extract was filtered through a 0.45 µm filter, phenolic analysis was carried out using a high‐performance liquid chromatography system (1260 Infinity; Agilent Technologies, Waldbronn, Germany) connected to a diode array detector (G1315D DAD) and equipped with a reversed‐phase C18 column (250 mm*4.6 mm, 5 µm, YMC Pack, Agilent Technologies, Santa Clara, CA, USA).…”

Effect of in vitro gastrointestinal digestion on phenolic compounds and antioxidant properties of soluble and insoluble dietary fibers derived from hulless barley

“…Phenolic compounds are health‐promoting phytochemicals in plants due to their well‐documented antioxidative, anti‐inflammatory, anticarcinogenic, antimutagenic, and antiviral activities (Devi, Vijayabharathi, Sathyabama, Malleshi, & Priyadarisini, 2014; Dueñas et al., 2016; Pozuelo et al., 2012; Villanueva‐Carvajal, Bernal‐Martínez, García‐Gasca, & Dominguez‐Lopez, 2013). Depending on their solubility characteristics, phenolic compounds occur in soluble and insoluble‐bound forms (Chandrasekara & Shahidi, 2010; Li et al., 2018). Soluble phenolic compounds are generally extracted by aqueous or aqueous‐organic solvent mixtures such as methanol–water or acetone–water (Acosta‐Estrada, Gutiérrez‐Uribe, & Serna‐Saldívar, 2014; Devi et al., 2014; Pellegrini et al., 2017a).…”

“…f.) has been identified as a rich source of DF and phenolic compounds as it is grown under harsh environments (Liang et al., 2017). Li et al., 2018) and Yang, Dang, & Fan, 2018 have reported that a large number of polyphenols with effective antioxidant activities were determined in different colored Tibetan hulless barley and cultivated Qinghai‐Tibet highland barley varieties. Our research team has reported that the contents of insoluble‐bound phenolics are 14.30 ± 0.04 and 5.90 ± 0.67 µmol ferulic acid equivalents (FAE)/g dry sample (DS) for IDF and SDF derived from hulless barley, respectively (Li et al., 2019).…”