Comparative Evaluation of the Antibacterial Efficacy of Aloe Vera, 3% Sodium Hypochlorite, and 2% Chlorhexidine Gluconate Against Enterococcus faecalis: An In Vitro Study

Abstract

AimTo compare the antibacterial efficacy of irrigants (aloe vera, 3% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), and saline) against Enterococcus faecalis using the turbidometric analysis and colony count method.Materials and methodsEighty freshly extracted, single-rooted, human mandibular premolar teeth were taken. Access opening was done, instrumented, and autoclaved. Samples were inoculated with 10 µL of an Enterococcus faecalis (E. faecalis) bacterial suspension and incubated at 37◦C for three days. Samples were divided into four groups of 20 teeth each based on the type of irrigating solution used. Group 1 was irrigated with 3% sodium hypochlorite; Group 2 with 2% chlorhexidine; group 3 with aloe vera; and Group 4 with 0.9% saline (the control group). Ten teeth from each group were subjected to a turbidity analysis by spectrophotometer and the remaining 10 teeth from each group were tested for colony-forming units (CFU)/mL. The plates were incubated at 37◦C for 24 hours and CFU that were grown were counted using a bacterial colony counter. Results were subjected to an analysis of variance (ANOVA) followed by a post hoc Games-Howell test.ResultsAll the tested irrigating solutions demonstrated an antibacterial effect against E. faecalis. The greatest antimicrobial effects were observed in samples treated with 2% CHX (p<0.001). No statistically significant difference was found between 3% NaOCl and aloe vera (p > 0.001) against E. faecalis.ConclusionTwo percent chlorhexidine exhibited good antimicrobial efficacy against E. faecalis. Three percent NaOCl and aloe vera showed a similar antimicrobial efficacy against E. faecalis. Aloe vera can be used as an antibacterial agent in novel drugs for the treatment of bacterial diseases.

In vitro evaluation of antibacterial effect of combination of honey and Aloe vera extract against Enterococcus faecalis and Escherichia coli

“…The results of colony count showed that H-Av mixture at a concentration of 25% completely inhibited the growth of the studied bacteria. In studies examining the antibacterial effect of Aloe vera and chlorhexidine against E.faecalis, chlorhexidine has shown better results than aloe vera (24)(25)(26). In the agar well diffusion test, H-Av mixture at a concentration of 25% did not show significant difference in the diameter of inhibitory zone compared to 2% chlorhexidine.…”

Efektivitas Antibakteri Hidrogel Lidah Buaya pada Treponema denticola dan Tannerela forsythia Bakteri: In Vitro

“…Pada penelitian ini, chlorhexidine bertindak sebagai kontrol positif. Menurut (Goud et al, 2018), chlorhexidine adalah antibakteri yang baik dengan toksisitas minimal. Pada konsentrasi rendah, bahan ini berefek bakteriostatik.…”

“…Secara tradisional, Aloe vera telah digunakan untuk penyembuhan luka, antiinflamasi dan antimikroba (Sánchez et al, 2020). Pada penelitian ini, efektifivitas antibakteri dari hydrogel loe vera pada bakteri Treponema denticola dan Tannerela forsythia dapat disebabkan oleh senyawa-senyawa aktif yang terkandung didalamnya yang berkhasiat sebagai antibakteri antara lain asam p-kumarat, asam askorbat, pirokatekol, dan asam sinamat (Goud et al, 2018). Sel parenkim dalam lidah buaya mengeluarkan gel mucilaginous yang tidak berwarna yang mengandung 98-99% air dan 1-2% senyawa aktif seperti aloesin, aloin, aloe-emodin, aloe-mannan, flavonoid, saponin, sterol, asam amino, tanin, dan vitamin.…”

Influência De Substâncias Irrigadoras Endodônticas Na Microdureza Da Dentina Radicular - Revisão Integrativa

“…Assim, o gluconato de clorexidina (CHX) tem sido proposto como um substituto para o NaOCl. O CHX não pode dissolver o tecido pulpar, mas possui uma excelente capacidade antimicrobiana e substantividade que mantêm um efeito antibacteriano por longos períodos de tempo 2,5 .…”