Comparative evaluation of the antimicrobial profile of Moringa leaf and seed oil extracts against resistant strains of wound pathogens in orthopedic hospitals

Abstract

The antimicrobial profile of oil extracts from Moringa oleifera leaves and seeds on orthopaedic wound pathogens was tested and compared with the antimicrobial activity of some antibiotics. The pathogens were characterized using biochemical and morphological tests. Antimicrobial susceptibility test was done on the pathogens using paper discs diffusion method. Plasmid curing was done on the isolates that showed resistance to antibiotics and the Moringa extracts. Time of kill assay was done with modified plating technique. Staphylococcus aureus accounted for 43% of the pathogens followed by Proteus spp. (16%), Klebsiella spp. (15%), Citrobacter spp. (11%), Escherichia coli (8%) and Pseudomonas aeruginosa (6%). Over 80% of the organisms were resistant to the tested antibiotics and their resistance were of plasmid origin. The methanolic leaf oil extract showed highest antimicrobial activity. The activity of the aqueous seed oil extract was significantly higher than the methanolic and ethanolic seed oil extracts (P > 0.05). Viable cell counts of S. aureus and Klebsiella spp. were reduced by the methanolic leaf and aqueous seed oil extracts. The antimicrobial activity of the methanolic leaf and aqueous seed oil extracts compared favourably with the reference antibiotics and can be used as alternatives for treatment of orthopaedic wound infections.

The potential effect of Moringa oleifera ethanolic leaf extract against oxidative stress, immune response disruption induced by abamectin exposure in Oreochromis niloticus

“…Only four of the thirteen species are the subject of current research (Moringa oleifera, Moringa stenopetala, Moringa concanensis, and Moringa peregrina) (Abd Rani et al 2018). Moringa oleifera Lam is the most prominent species of the Moringa genus; it contains a variety of beneficial phytochemical substances such as phenolic and flavonoid compounds (Sankhalkar and Vernekar 2016), alkaloids, tannins, triterpenoids, sterols, isothiocyanate (Leone et al 2015), and moringyne (Dalei et al 2016;Ganatra et al 2012;Oyedara et al 2021) with potent anthelmintic (Hegazi et al 2018), antiviral (Biswas et al 2020;Xiong et al 2022), and antibacterial effects (Anthony et al 2017;El-Kholy et al 2018;Fouad et al 2019).…”

Potential impacts of edible coatings fortified by Moringa and /or Cedar extracts on quality and shelf life of chilled turkey fillets

“…The native Indian plant Moringa oleifera, (M. oleifera) usually called moringa, is valued for its high medical merits because of its curative qualities (Chandrappa et al 2015). They are also considered rich sources of several phytochemical substances, such as glucosinolates (Mgbeahuruike et al 2017). Known by most as drumstick, M. oleifera contains various active ingredients, including triterpenoids, alkaloids, tannins, flavonoids, and saponins (Wansi et al, 2013).…”