Comparative evaluation of the clonogenic capacity of periodontal ligament fibroblasts in Hank's balanced salt solution and egg albumen: An in vitro study

Shah, Dimpi; Sulkshane, Prasad; Lalwani, Rutika; Pawar, Sagar; Teni, Tanuja; Kakade, Adesh

doi:10.1111/edt.12408

Cited by 2 publications

(1 citation statement)

References 30 publications

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“…The first form of evidence of cell growth was seen within four weeks of culture, and the cells were about 80% confluent by the eighth week of culture. The cells that grew were elongated and spindle-shaped, and they attained a whirled pattern at confluence, just as described in previous studies 18,27 . The cells were passaged into T 75 culture flasks for further exponential growth and elimination of other possible cell types.…”

Section: Isolation and Cell Culturesupporting

confidence: 72%

Viability of periodontal ligament cells in selected transport media for avulsed teeth

Williams,

Denloye,

Popoola

et al. 2023

Braz. J. Oral Sci.

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To compare the viability of periodontal ligament (PDL) cells stored in Hanks Balanced Salt Solution (HBSS) with those in readily available transport media over a variable period of time. Methods: Periodontal ligament cells harvested from premolars freshly extracted for orthodontic reasons were cultured for exponential growth. The cells were exposed to egg white, evaporated milk, water and Hanks Balanced Salt Solution (HBSS) at room temperature. Their viability was evaluated after 30 minutes, 1 hour and 3 hours with the tetrazolium salt-based colorimetric assay (MTT assay). Statistical analysis was done using the IBM® SPSS version 23.0 software. Comparison between the Mean Optical Densities (MODs) of the cells stored in HBSS and other media at each time interval was done using the independent t test. Repeated measure ANOVA and Tukey post-hoc test were also carried out to compare the MOD of cells within each medium over time. The level of significance was set at p <0.05. Result: The PDL cells stored in egg white had higher MODs than those in HBSS at 30 minutes and 1 hour. Conversely, the MODs of the cells stored in milk and water were lower than those in HBSS at all the studied points. There was a significant difference in the viability of the cells stored in HBSS and water at all the time points (p<0.05). Conclusion: For up to an hour, egg white was found to perform better than HBSS in supporting the viability of PDL cells.

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Section: Isolation and Cell Culturesupporting

confidence: 72%

Viability of periodontal ligament cells in selected transport media for avulsed teeth

Williams,

Denloye,

Popoola

et al. 2023

Braz. J. Oral Sci.

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Solutions for the preservation of injured teeth: a systematic review

Mitkina,

Strelnikova,

Gorbatova

et al. 2024

Russian Journal of Dentistry

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Rationale: Traumatic dental injuries resulting from mechanical impact account for approximately 20% of oral and maxillofacial system diseases. Currently, the method of replantation presents a viable alternative to prosthetics and implantation. The success of replantation is largely determined not only by the speed at which the patient seeks dental care but also by the conditions under which the avulsed tooth is transported. Objective: To search for and analyze scientific literature on solutions that can preserve the viability of teeth following traumatic injuries. Materials and methods: PubMed and eLibrary databeses were utilized for the literature search. The review included studies that analyzed the biological properties of solutions used to maintain the viability of traumatized teeth. After screening and analyzing the full-text articles, 36 articles focused on comparing the efficacy of various transport media were included in the review. Results: In the reviewed literature, we identified 34 different solutions, with their effectiveness evaluated by counting viable periodontal cells. The most frequently studied solutions were HBSS, various types of milk, propolis, and coconut water. The studies demonstrated significant differences in the effectiveness of the solutions used, storage conditions, and methods for diagnosing cell viability. Laboratory tests such as mtt and trypan blue were most commonly used to assess metabolic activity. HBSS and milk are the most effective media for preserving the viability of tooth tissues. Conclusion: The research findings on the efficacy of solutions for maintaining the viability of cells in traumatized teeth highlight the need for the development of more accessible and effective transport media due to contradictory information regarding their biological effectiveness and indicate potential avenues for further research.

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Comparative evaluation of the clonogenic capacity of periodontal ligament fibroblasts in Hank's balanced salt solution and egg albumen: An in vitro study

Abstract: Due to its limited ability to support the clonogenicity of PDLF's, egg albumen is a poor storage medium for an avulsed tooth compared to either DMEM or HBSS.

Cited by 2 publications

References 30 publications

Viability of periodontal ligament cells in selected transport media for avulsed teeth

Viability of periodontal ligament cells in selected transport media for avulsed teeth

Solutions for the preservation of injured teeth: a systematic review

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