Comparative evaluation of the effect of decontamination protocol on the shear bond strength of eighth generation bonding agent to contaminated dentin: an in vitro study

Abstract

Introduction. This in vitro study aims to evaluate and compare the effect of decontamination protocol - based on sodium hypochlorite - NaOCl, as a decontaminating agent, followed by the application of a collagen cross-linking agent like sodium ascorbate [Na Ascorbate] or Proanthocyanidin [PA]) on the shear bond strength of eighth generation bonding agent to contaminated dentin (saliva, blood and blood followed by hemostatic agent).
 Methodology. The buccal surfaces of extracted premolars were flattened to expose the dentine. The teeth were then randomly divided into 3 groups based on the contaminant used (saliva, blood and blood followed by hemostatic agent). Decontamination protocol was carried out with sodium hypochlorite followed by application of either of the two collagen cross-linking agents (sodium ascorbate/proanthocyanidin) in each group. FuturaBond DC (eighth generation) bonding agent was used to bond composite to dentin surfaces which was subjected to shear bond strength testing. Data was analyzed by one-way ANOVA and Tukey test.
 Results All the groups (contaminated with saliva, blood, or hemostatic agent) showed a decrease in shear bond strength of composite resin to dentin. The shear bond strength of composite resin to dentin was least affected by contamination with saliva followed by contamination with blood and was most affected by contamination with a hemostatic agent.
 The decontamination protocol carried out showed that the combination of NaOCl and Proanthocyanidin improved the shear bond strength of composite to dentin significantly more than the combination of NaOCl and sodium ascorbate or ofsodium hypochlorite used alone.

Comparative evaluation of different surface treatments of the salivary contaminated dentin on the shear bond strength of self-etch adhesives: An in vitro study

“…Ethanol also reduces the surface tension of water present in the saliva and allows the spreading of the mixture along the surfaces that are coated with water. [ 14 ] This could be the reason for improved bond strength after using ethanol as a decontaminant in the present study. Our results are in agreement with a study by Yaman et al .…”

Avaliação da efetividade da aplicação de etanol previamente ao adesivo universal sob os diferentes terços dentinários

“…Levando em conta os fatores abordados previamente, ainda a literatura apresenta falta de estudos e consenso relacionado as estrategias para melhorar o desempenho adesivo em dentina, principalmente quando relaionado aos adesivos universais, principalmente relaconado a técnica de associação ao etanol . Além disso, a maioria dos estudos tem avaliado a adesão de materiais resinosos à dentina como um todo, sem considerar a influência da morfologia dentinária (profundidade em relação à câmara pulpar), que se torna mais complexa conforme sua profundidade na cavidade Sadek et al, 2008;Breschi et al, 2019;Yi L et al, 2019;Kewlani et al, 2020; Outro fator importante a ser considerado ao avaliar os sistemas adesivos está relacionado ao envelhecimento/longevidade. É importante avaliar o sistema adesivo a longo prazo (> 1 ano ou mais de uso, simulado com armazenamento de 1 ano em água / saliva ou termociclado, por exemplo) .…”