Comparative Evaluation of the Loop-Mediated Isothermal Amplification Assay for Detecting Pulmonary Tuberculosis

Kim, Chang Ki; Cho, Eunah; Shin, Dong Mi; Choi, Sung Won; Shin, So Youn

doi:10.3343/alm.2018.38.2.119

Cited by 13 publications

(7 citation statements)

References 23 publications

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“…TB-LAMP inferiority concerning sensitivity compared with Xpert is probably explained by the lower volume of sputum used in the test (60 μL for TB-LAMP versus 1000 μL for Xpert) [11]. The superiority of TB-LAMP compared with smear microscopy is highlighted in our study by the finding that approximately 50% of smear-negative samples were TB-LAMP-positive, as previously observed [3,[12][13][14][15]. In addition to the sensitivity, TB-LAMP could distinguish fairly well between MOTT and MTB; hence, it avoids inappropriate anti-TB treatment for patient with NTM infection, which is observed around 10.8% and 5.6% among patients with presumptive TB and all smear-positive cases, respectively [16].…”

Section: Discussionsupporting

confidence: 77%

Evaluation of Loopamp Assay for the Diagnosis of Pulmonary Tuberculosis in Cambodia

Cheng

Pheng

Heng

et al. 2020

BioMed Research International

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The Loopamp™ MTBC kit (TB-LAMP) is recommended by WHO for Mycobacterium tuberculosis complex detection in low-income countries with a still low drug-resistant tuberculosis (TB) rate. This study is aimed at testing its feasibility in Cambodia on sputa collected from presumptive tuberculosis patients. 499 samples were tested at a smear microscopy center and 200 at a central-level mycobacteriology laboratory. Using mycobacterial cultures as reference, TB-LAMP results were compared with those of LED fluorescent microscopy (LED-FM) and Xpert® MTB/RIF. At the microscopy center, TB-LAMP sensitivity was higher than that of LED-FM (81.5% [95% CI, 74.5-87.6] versus 69.4% [95% CI, 62.2-76.6]), but lower than that of the Xpert assay (95.5% [95% CI 92.3-98.8]). At the central-level laboratory, TB-LAMP sensitivity (92.8% [95% CI, 87.6-97.9]) was comparable to that of Xpert (90.7% [95% CI, 85.0-96.5]) using stored sample. No significant difference in terms of specificity between TB-LAMP and Xpert assays was observed in both study sites. In conclusion, our data demonstrate that TB-LAMP could be implemented at microscopy centers in Cambodia to detect TB patients. In addition, TB-LAMP can be a better choice to replace smear microscopy for rapid TB diagnosis of new presumptive TB patients, in settings with relative low prevalence of drug-resistant TB and difficulties to implement Xpert assay.

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Section: Discussionsupporting

confidence: 77%

Evaluation of Loopamp Assay for the Diagnosis of Pulmonary Tuberculosis in Cambodia

Cheng

Pheng

Heng

et al. 2020

BioMed Research International

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“…According to previous studies, both had shown comparable performance in smear-positive samples, but higher sensitivity was shown in Xpert MTB/RIF than in the LAMP test [ 6 , 12 ]. Xpert MTB/RIF has been endorsed for use in the diagnosis of TB in many countries, including Thailand [ 4 , 31 ]. However, only a portion of patients, excluding foreigners and ethnic minorities, could reimburse the cost for Xpert MTB/RIF due to the regulation stated by The National Health Security Office (NHSO).…”

Section: Discussionmentioning

confidence: 99%

“…To better control the spread of TB, access to rapid diagnostic tools should be provided to all patients with symptoms suggestive of TB [3]. Thus, a LAMP assay may be more applicable in terms of accessibility and affordability, especially in the decentralized areas [4,32].…”

Section: Plos Onementioning

confidence: 99%

“…However, TB is still underdiagnosed and undertreated, especially in resource-limited countries, due to the lack of highly sensitive and specific diagnostic tools which are usually expensive and require adequate infrastructure [1,3]. Novel diagnostic methods with enough simplicity and cost-effectiveness are therefore necessary to improve the accurate identification of TB patients in those resource-limited settings [3,4].…”

Section: Introductionmentioning

confidence: 99%

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Pragmatic accuracy of an in-house loop-mediated isothermal amplification (LAMP) for diagnosis of pulmonary tuberculosis in a Thai community hospital

et al. 2020

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Background To improve the quality of diagnosing pulmonary tuberculosis (TB), WHO recommends the use of rapid molecular testing as an alternative to conventional microscopic methods. Loopmediated isothermal amplification assay (LAMP test) is a practical and cost-effective nucleic amplification technique. We evaluated the pragmatic accuracy of an in-house LAMP assay for the diagnosis of TB in a remote health care setting where an advanced rapid molecular test is not available. Methods A prospective diagnostic accuracy study was conducted. Patients with clinical symptoms suggestive of TB were consecutively enrolled from April to August 2016. Sputum samples were collected from each patient and were sent for microscopic examination (both acid-fast stain and fluorescence stain), in-house LAMP test, and TB culture. Results One hundred and seven patients with TB symptoms were used in the final analysis. This included 50 (46.7%) culture-positive TB patients and 57 (53.3%) culture-negative patients. The overall sensitivity of the in-house LAMP based on culture positivity was 88.8% (95/107) with a 95%CI of 81.2-94.1. The sensitivity was 90.9% (40/44) with a 95%CI of 78.3-97.5 for smear-positive, culture-positive patients, and was 16.7% (1/6) with a 95%CI of 0.4-64.1 for smear-negative, culture-positive patients. The overall sensitivity of the in-house LAMP test compared to smear microscopy methods were not significantly different (p = 0.375). The

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“…Notomi et al [14] first introduced a method to amplify DNA in an isothermic process called Loop-Mediated Isothermal Amplification (LAMP) in 2000. The method boasts simplicity and efficiency compared to PCR by requiring only a constant temperature of around 60-65 °C and has a comparable diagnostic accuracy, if not superior, to smear microscopy [12,15,81]. This method's high specificity is owed to four primers' DNA recognition at the start of the amplification and two at the next stage [14].…”

Section: Loop-mediated Isothermal Amplification and The Working Principlementioning

confidence: 99%

Combining LAMP and Au-Nanoprobe to detect INH-RIF resistance accurately in tuberculosis: an evidence-based review

Habiburrahman

Ariq

Handayani

2021

J Infect Dev Ctries

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Approximately 1.41 million people die annually due to tuberculosis. One of the main problems in Tuberculosis eradication is the development of resistance to various antibiotics. However, current efforts to detect resistances face challenges such as limited equipment, budget, and time. This evidence-based review investigated loop-mediated isothermal amplification, an alternative molecular diagnostic tool with promising performance and applicability in developing countries, and its use combined with Au-Nanoprobe to detect antibiotic resistance in tuberculosis. The literature search was conducted through four databases (Proquest, EBSCOhost, Scopus, and Pubmed) for useful articles on loop-mediated isothermal amplification and Au-Nanoprobe in detecting tuberculosis and tuberculosis resistance. After filtering the result with inclusion and exclusion criteria, the search produced three papers that best answer the clinical question. Loop-mediated isothermal amplification amplifies a target sequence, and Au-Nanoprobe responds to the DNA specific to the target mutant, producing an observable color change. Loop-mediated isothermal amplification and Au-Nanoprobe showed 100% sensitivity and specificity in detecting rifampicin and isoniazid resistance. Another study investigated its viability to detect tuberculosis and found 98.2% sensitivity and 88.2% specificity. Combining loop-mediated isothermal amplification and Au-Nanoprobe had a shorter time to get results and should also be relatively cheaper because it does not need a high temperature to work and requires less equipment. In conclusion, loop-mediated isothermal amplification and Au-Nanoprobe can be used as an efficient and accurate method to detect isoniazid and rifampicin-resistant tuberculosis strains. The new technology is promising for developing countries due to their high disease burden but facing several healthcare barriers.

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Comparative Evaluation of the Loop-Mediated Isothermal Amplification Assay for Detecting Pulmonary Tuberculosis

Cited by 13 publications

References 23 publications

Evaluation of Loopamp Assay for the Diagnosis of Pulmonary Tuberculosis in Cambodia

Evaluation of Loopamp Assay for the Diagnosis of Pulmonary Tuberculosis in Cambodia

Pragmatic accuracy of an in-house loop-mediated isothermal amplification (LAMP) for diagnosis of pulmonary tuberculosis in a Thai community hospital

Combining LAMP and Au-Nanoprobe to detect INH-RIF resistance accurately in tuberculosis: an evidence-based review

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