Comparative evaluation of the metabolic potentials of different strains of Peptostreptococcus productus: utilization and transformation of aromatic compounds

Abstract

Three strains of Peptostreptococcus productus were tested for growth at the expense of methoxylated aromatic compounds. Strain M8A-18 (human fecal isolate) was unable to utilize methoxylated aromatic compounds. While the type strain ATCC 27340 (human septicemia isolate) was capable of minimal growth with methoxylated aromatic compounds, ATCC 35244 (sewage sludge isolate) displayed significant growth on methoxylated aromatic compounds. Methoxylated phenols, benzoates, benzyl alcohol and phenylacrylates supported the growth of ATCC 35244 and were O-demethylated to their respective hydroxylated derivatives. During O-methyl- or CO-dependent growth, the double bond of the acrylate side chain of certain methoxylated and non-methoxylated phenylacrylates was reduced. Although other aromatic substituent groups (-COOH and -CH3) were transformed during CO-dependent growth, in short-term growth studies, the aromatic ring was not subject to reduction or degradation. Of the three strains tested, only strain M8A-18 failed to grow at the expense of carbon monoxide (CO).

Metabolism of aromatic aldehydes as cosubstrates by the acetogen Clostridium formicoaceticum

“…Since acetogenic bacteria utilize aromatic O-methyl groups (Bache and Pfennig 1981;Krumholz and Bryant 1985;Wu et al 1988;Daniel et al 1991;Kreft and Schink 1993;El Kasmi et al 1994;Kaufmann et al 1997), acrylate groups (Bache and Pfennig 1981;Tschech and Pfennig 1984;Parekh et al 1992;Lux and Drake 1992;Misoph et al 1996), carboxyl groups (Hsu et al 1990a, b), and aldehyde groups (Gößner et al 1994) for carbon, energy, and growth, they are probably involved in the anaerobic degradation of lignin-derived monomers and aromatic pollutants in certain environments (Kirk and Farrell 1987;Young and Frazer 1987;Evans and Fuchs 1988;Neilson et al 1991;Schink et al 1992;Taraban et al 1993;Diekert and Wohlfarth 1994;Drake 1994;Drake et al 1997). Under in situ conditions, it is very likely that most acetogens grow at the expense of complex mixtures of substrates.…”

Biodegradación anaerobia de compuestos petroquímicos

“…Tschech y Pfennig (1984), reportaron el crecimiento de Acetobacterium woodii asociado a la reducción de cafeato, en donde la parte acrílica de este compuesto se reduce a hidrocafeato utilizando como donadores de electrones al metanol o formiato, vainillato o ferulato. Sin embargo, otra bacteria anaerobia estricta (Peptostreptococcus productus) en presencia de CO, redujo la cadena acrílica de algunos compuestos aromáticos pero no utilizó el ácido acrílico, por lo que concluyen que la capacidad de los microorganismo para reducir la cadena acrílica de un aromático no esta relacionada a la habilidad para utilizar el ácido acrílico (Parekh et al, 1992).…”