Comparative evaluation of the sealing ability of filling materials on root end cavities treated with smear layer removing agents: A confocal laser scanning microscopic study

Abstract

Aim: This study aims to evaluate and compare the sealing ability of Biodentine™ and mineral trioxide aggregate (MTA) plus ® on root end cavities treated with 17% ethylene diamine tetraacetic acid (EDTA), 0.2% Chitosan and 1% Phytic acid using Confocal Laser Scanning Microscope (CLSM)-An in vitro study. Materials and Methods: Sixty extracted single rooted teeth were instrumented and obturated with gutta-percha. The apical 3 mm of each tooth was resected and 3 mm root-end preparation was made using ultrasonic tip. 17% EDTA ( n = 20), 0.2% Chitosan ( n = 20) and 1% Phytic acid ( n = 20) was used as a smear layer removing agent and each above group was further subdivided and restored with a root end filling material, Biodentine ( n = 10) and MTA Plus ( n = 10). The samples were coated with varnish except at the root end and after drying, they were immersed in Rhodamine B dye for 24 h. The teeth were then rinsed, sectioned longitudinally, and observed under CLSM. Results: In the present study, MTA Plus® treated with 1% Phytic acid showed the least microleakage followed by Biodentine™ treated with 1% Phytic acid which was statistically not significant. MTA Plus ® treated with 17% EDTA showed the highest microleakage when compared to other tested groups. There was a significant difference in microleakage between MTA Plus® and Biodentine™ when treated with 17% EDTA and 0.2% Chitosan. However, more microleakage was seen with Biodentine™ group than MTA plus® group. Conclusion: Root end cavities restored with MTA plus and treated with Phyitc acid showed superior sealing ability. Furthermore, smear layer removing agents will aid in better adaptability of root end filling material.

Comparison of push‐out bond strength and apical microleakage of different calcium silicate‐based cements after using EDTA, chitosan and phytic acid irrigations

“…Shah et al (2019), BD and MTA Plus materials were applied to samples prepared with retrograde cavity principles, which were irrigated with 17% EDTA, 0.2% chitosan and 1% phytic acid, and evaluated the apical microleakage with Rhodamine B dye. As a result of the study, it was reported that 1% phytic acid was the chelator with the lowest microleakage for both materials.…”

“…As a result of the study, it was reported that 1% phytic acid was the chelator with the lowest microleakage for both materials. Shah et al (2019) reported that the possible reason for this finding may be due to the strong binding affinity of phytic acid to important minerals such as calcium, iron and zinc. They also noted that binding of calcium with phytic acid is pH dependent, which may lead to better chelating ability than conventional chelating agents.…”

Evaluation of the Effect of Different Chelation Agents on the Bond Strength of Filling Materials in Retrograde Cavities: An In Vitro Study

“…Literatürde, kitosanın endodontide şelasyon ajanı olarak kullanımıyla ilgili sınırlı sayıda çalışma mevcuttur. 24,25 Silva ve ark., yaptıkları çalışmada, farklı konsantrasyonlardaki kitosanın 3 ve 5 dk'lık uygulamadan sonra smear tabakası üzerindeki etkilerini değerlendirmişlerdir. Bu çalışmanın sonucunda 3 dk boyunca uygulanan %0,2 kitosanın, smear tabakası uzaklaştırma etkinliğinin %15 EDTA ve %10 sitrik asit ile benzer bir etki gösterdiği görülmüştür.…”