Background and objectives: Controlling mechanical plaque is a crucial component of periodontal treatment. In the current research, the effectiveness of using ozone water irrigation in addition to scale and root planing to treat generalised chronic periodontitis was assessed.Materials and methods: After scaling and root planing, the twenty-four chronic periodontitis patients chosen for the research were randomly assigned to Group A or Group B, getting irrigation with ozone water or distilled water, respectively. To assess the "red complex" periodontal pathogens, subgingival plaque was taken from the chosen investigational teeth and examined using the BANA-ZymeTM Processor. At baseline, 14 days, 21 days, and 2 months, the clinical and microbiological data were noted.Results: At the baseline, Group A and Group B's mean probing pocket depth scores were 6.833 1.193 and 7.833 1.276, respectively; on Day 14, they were 6.616 1.403 and 7.083 1.378; on Day 21, they were 5.166 0.937 and 6.083 1.443; and on Month 2, they were 4.500 0.797 and 5.166 1.029. In Group A, 9 samples at the second month showed BANA negativity, 3 samples showed BANA positivity, and in Group B, 12 samples at the second month showed BANA negativity, 0 samples showed BANA positivity. In both groups, the microbiological study revealed a decrease in periodontal infections.Conclusion: An effective complement to scaling and root planing in the treatment of chronic periodontitis, subgingival ozonated water irrigation has shown significant improvement in both clinical and microbiological indices.