Comparative expression analysis reveals differences in the regulation of intestinal paraoxonase family members

Precourt, Louis Philippe; Seidman, Ernest G.; Delvin, Edgard; Amre, Devendra; Deslandres, Colette; Dominguez, Michel; Sinnett, Daniel; Lévy, Émile

doi:10.1016/j.biocel.2009.02.013

Cited by 38 publications

(50 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Iron-Ascorbate Exposure-To determine infected and untreated cell response to oxidative stress, they were exposed to iron (0.2 mM)-ascorbate (2 mM) (1:10) system-mediated lipid peroxidation (29,30). At the end of a 6-h culture period, the degree of lipid peroxidation was evaluated by measuring MDA in cells.…”

Section: Estimation Of Lipid Peroxidationmentioning

confidence: 99%

Modification in Oxidative Stress, Inflammation, and Lipoprotein Assembly in Response to Hepatocyte Nuclear Factor 4α Knockdown in Intestinal Epithelial Cells

Marcil

Seidman

Sinnett

et al. 2010

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Estimation Of Lipid Peroxidationmentioning

confidence: 99%

Modification in Oxidative Stress, Inflammation, and Lipoprotein Assembly in Response to Hepatocyte Nuclear Factor 4α Knockdown in Intestinal Epithelial Cells

Marcil

Seidman

Sinnett

et al. 2010

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…LPS gibi proinflamatuar ajanların insan bağır-sak Caco-2/15 hücrelerinde PON2 mRNA ekspresyonunu düşerken protein miktarlarında artış olduğunu göstermiştir [24]. Çalışmamızda 4 saat LPS uygulanan KHDAK hücre hatlarında PON2 protein seviyelerinde artış gözlemlenmemiştir.…”

Section: Discussionunclassified

“…Precourt ve arkadaşları [24] Caco-2/15 hücre bağır-sak hattında demir-askorbat aracılı oluşturulan lipit peroksidasyonunun malondialdehit (MDA) düzey-lerini arttırdığını ve PON ekspresyonunu azalttığını, ek olarak PON3 protein miktarı değişmeden PON2 miktarının azaldığını göstermişlerdir. Bu hücrelerin troloks gibi güçlü antioksidanlarla pre-inkübasyonu PON ekspresyonlarındaki azalmayı durdurur.…”

Section: Discussionunclassified

Akciğer kanseri hücre dizilerinde hipoksi indüklenebilir faktör-1 (HIF-1) ve paraoksonaz enzim ilişkisinin araştırılması

Karagenc¹,

Meydancı²,

Kucuksayan³

2015

Dicle Med J

View full text Add to dashboard Cite

Objective: In this study, it was aimed to investigate that PON2, in hypoxia and normoxia in relation to HIF-1α transcription factor that has a role in tumour angiogenesis. Hypoxia inducible factor 1 alpha (HIF-1α) is a transcription factor which is sensitive to hypoxia, causes initiation of angiogenesis and metastasis by providing transcription of numerous genes. As well as hypoxia several inflammatory agents such as lipopolysaccharide (LPS) can regulate the expression of HIF-1α as well as PON2. Oxidative stress is known to have role in cancer. Paraoxonase 2 which is one of the members of paraoxonase family serves as intracellular anti-oxidant.Methods: H1299, A549 ve PC14 non-small cell lung carcinoma cell lines used in this study. Cells were cultured under hypoxia and normoxia conditions with LPS stimulation. HIF-1α and PON2 mRNA expression levels measured by real-time PCR. Western blot studies were performed for protein expression. Results:In this study, it was observed that LPS treatment stimulates HIF-1α expression which increases PON2 expression in NSCLC cell line in under hxpoxia conditions. Conclusion:This study shows that PON2 is regulated by HIF-1α in hypoxia and inflammation. The relationship between hypoxia and inflammation and oxidative status of cells requires further studies.Key words: HIF-1α, PON2, LPS, Hypoxia ÖZET Amaç: Bu çalışmada tümör anjiyogenezinde önemli rol oynayan hipoksi indüklenebilir faktör 1 (HIF-1) transkripsiyon faktörünün hipoksik ve normoksik koşullarda paraoksonaz 2 (PON2) ile ilişkisinin araştırılması hedeflenmiştir. Hipoksiye duyarlı bir transkripsiyon faktör olan HIF-1α çok sayıda genin transkripsiyonunu sağlayarak anjiyogenez ve metastazın başlamasına yol açar. Hipoksinin yanı sıra lipopolisakkarit (LPS) gibi çeşitli inflamatuvar ajanlar da HIF-1 α'nın ve PON2 nin ekspresyonunu düzenleyebi-lirler. Oksidatif stres karsinogenezde önemli etiyolojik faktörlerden birisidir. Paraoksonaz ailesi üyelerinden paraoksonaz 2 (PON2) nin hücre içi antioksidan olarak görev yaptığı bilinmektedir.Yöntemler: H1299, A549 ve PC14 akciğer hücre dizileri normoksi ve hipoksi ortamlarında kültürü yapılarak ve lipopolisakkaritle indüklenerek HIF-1 ve PON2 mRNA ekspresyonları ve western blot tayinleri çalışılmıştır. Bulgular: Çalışma sonucunda LPS uygulamasının HIF-1α ekspresyonunu uyardığı ve hipoksi koşullarında akciğer hücre dizilerinde PON2 ekspresyonunun arttığı göz-lendi.Sonuç: Bu çalışmada PON 2 enzim ekspresyonunun HIF-1α aracılığıyla hipoksi ve normoksi koşullarında regüle edildiği gözlenmiş olup hipoksi, inflamasyon ve hüc-renin oksidatif durumu arasındaki ilişkinin aydınlatılması için ileri çalışmalar gerekebilir. Anahtar kelimeler: HIF-1α, PON2, LPS, Hipoksi GİRİŞBu çalışmada akciğer kanseri hücre hatları kullanı-larak, tümör anjiyogenezinde önemli bir rol oynayan hipoksi indüklenebilir faktör 1 transkripsiyon faktörünün (HIF-1α) hipoksik ve normoksik koşullarda PON 2 enzimiyle ilişkisinin araştırılması amaçlanmıştır.

show abstract

“…Caco-2/15 cells were grown at 37°C with 5% CO2 in MEM, as described previously (21,23,26,27,33). PON2 gene expression was silenced by infection of these intestinal cells with lentivirus by a procedure also described in our previous studies (21,23,26,27,33).…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, oxidative stress and inflammation selectively affect the expression of PONs, which suggests their potential implication in IBD (33). PON1 activities have been found to be reduced in the serum of patients with active CD (4) and UC (3).…”

mentioning

confidence: 99%

Antioxidative properties of paraoxonase 2 in intestinal epithelial cells

Précourt

Marcil

Ntimbane

et al. 2012

American Journal of Physiology-Gastrointestinal and Liver Physi

Self Cite

View full text Add to dashboard Cite

Paraoxonase (PON) family members seem central to a wide variety of human illnesses, but appreciation of their antioxidative function in the gastrointestinal tract is in its infancy. The major objective of the present work is to highlight the role of the ubiquitously expressed PON2 in the small intestine. With use of pLKO lentiviral vector containing short hairpin RNA (shRNA) lentivirus, PON2 expression was knocked down in intestinal Caco-2/15 cells, where antioxidative status, lipid peroxidation, and degree of inflammation were evaluated. As a consequence of PON2 inactivation in the epithelial cells, we observed 1) imbalanced primary and secondary antioxidative responses, characterized by increased superoxide dismutases and decreased catalase, 2) high concentrations of H2O2 and malondialdehyde, along with low glutathione-to-glutathione disulfide ratio, 3) upregulation of TNF-α, IL-6, and monocyte chemoattractant protein-1 gene expression after induction of oxidative stress, and 4) raised level of the activation of transcription factor NF-κB, which was likely implicated in exacerbation of the inflammatory activation. These results suggest that PON2 is involved in the antioxidative and anti-inflammatory response in intestinal epithelial cells.

show abstract

Comparative expression analysis reveals differences in the regulation of intestinal paraoxonase family members

Cited by 38 publications

References 39 publications

Modification in Oxidative Stress, Inflammation, and Lipoprotein Assembly in Response to Hepatocyte Nuclear Factor 4α Knockdown in Intestinal Epithelial Cells

Modification in Oxidative Stress, Inflammation, and Lipoprotein Assembly in Response to Hepatocyte Nuclear Factor 4α Knockdown in Intestinal Epithelial Cells

Akciğer kanseri hücre dizilerinde hipoksi indüklenebilir faktör-1 (HIF-1) ve paraoksonaz enzim ilişkisinin araştırılması

Antioxidative properties of paraoxonase 2 in intestinal epithelial cells

Contact Info

Product

Resources

About