Comparative expression profile of micro<scp>RNA</scp>s and pi<scp>RNA</scp>s in three ruminant species testes using next‐generation sequencing

H, Wang; Zhong, Jincheng; Chai, Zhixin; Zhu, Jiangjiang; Xin, Jinwei

doi:10.1111/rda.13195

Cited by 15 publications

(12 citation statements)

References 47 publications

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“…To identify the novel miRNAs in indicine genome, 18687 mature miRNA sequences from 51 families of genus chordates were selected and mapped to the indicine genome. MiREAP was employed for extraction of potential new miRNA precursors [ 42 – 44 ]. Furthermore, the conserved new miRNA was subjected to phylogenetic analysis by MEGA7.0 [ 45 ].…”

Section: Methodsmentioning

confidence: 99%

In silico identification of conserved miRNAs and their selective target gene prediction in indicine (Bos indicus) cattle

et al. 2018

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The modern cattle was domesticated from aurochs, sharing its physiological traits into two subspecies Bos taurus and Bos indicus. MicroRNAs (miRNAs) are a class of non-coding short RNAs of ~22nt which have a key role in the regulation of many cellular and physiological processes in the animal. The current study was aimed to predict and annotate the potential mutations in indicine miRNAs throughout the genome using de novo and homology-based in silico approaches. Genome-wide mapping was performed in available indicine assembly by the homology-based approach and 768 miRNAs were recovered out of 808 reported taurine miRNAs belonging to 521 unique mature miRNA families. While 42 precursors were dropped due to lack of secondary miRNA structure, increasing stringency or decreasing similarity between the two genomes’ miRNA. Increasing tendency of miRNAs incidence was observed on chr5, chr7, chr8, chr12 and chr21 with 19 polycistronic miRNA within 1-kilobase distance throughout the indicine genome. Notably, 12 miRNAs showed copy number variation. Eighteen miRNAs showed a mutation in their mature sequences in which eight were found in their seed region. Whilst in de novo based approach, 12 novel potential miRNAs on Y chromosome in indicine cattle along with a new miRNA (bind-miR-1264) on chrX were found. The final data set is annotated and explains the impending target genes that are responsible for enhanced immunity, heat tolerance and disease tolerance regulation in indicine. The study conforms to better understanding and perceptive approach towards indicine genome.

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Section: Methodsmentioning

confidence: 99%

In silico identification of conserved miRNAs and their selective target gene prediction in indicine (Bos indicus) cattle

et al. 2018

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“…In bovine, several miRNAs were identified in male reproduction, which explored its differential expression in the process of spermatogenic arrest in cattleyak [21][22][23]. Moreover, some studies reported the expression of miRNA in bull sperm [24,25].…”

Section: Introductionmentioning

confidence: 99%

MiRNAs Expression Profiling of Bovine (Bos taurus) Testes and Effect of bta-miR-146b on Proliferation and Apoptosis in Bovine Male Germline Stem Cells

Gao

Ren

et al. 2020

IJMS

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Spermatogenesis is a complex biological process regulated by well-coordinated gene regulation, including MicroRNAs (miRNAs). miRNAs are endogenous non-coding ribonucleic acids (ncRNAs) that mainly regulate the gene expression at post-transcriptional levels. Several studies have reported miRNAs expression in bull sperm and the process of spermatogenic arrest in cattle and yak. However, studies for the identification of differential miRNA expression and its mechanisms during the developmental stages of testis still remain uncertain. In the current study, we comprehensively analyzed the expression of miRNA in bovine testes at neonatal (3 days after birth, <i>n</i> = 3) and mature (13 months, n = 3) stages by RNA-seq. Moreover, the role of bta-miR-146b was also investigated in regulating the proliferation and apoptosis of bovine male germline stem cells (mGSCs) followed by a series of experiments. A total of 652 miRNAs (566 known and 86 novel miRNAs) were identified, whereas 223 miRNAs were differentially expressed between the two stages. Moreover, an elevated expression level of bta-miR-146b was found in bovine testis among nine tissues, and the functional studies indicated that the overexpression of bta-miR-146b inhibited the proliferation of bovine mGSCs and promoted apoptosis. Conversely, regulation of bta-miR-146b inhibitor promoted bovine mGSCs proliferation. This study provides a basis for understanding the regulation roles of miRNAs in bovine testis development and spermatogenesis.

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“…To explain genetic adaptability, many studies focused on the expression differences levels among tissues of domestic yaks or on differences between domestic yaks and domestic cattle (Bos Taurus) living in different altitude areas (Song et al, 2019;H. Wang et al, 2018;Kun Wang et al, 2014;Zi et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptome analysis of winter yaks in plateau and plain

Wang

Guan

Kangzhu

et al. 2021

Reprod Domestic Animals

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Yaks (Bos grunniens) are important animals for the ecological environment and the people living in the Tibetan Plateau (D. D. Wu et al., 2018;J. Wu, 2016). The Maiwa yak population is widely distributed in the Northeast of the Tibetan Plateau and ranks as the second major yak population in China (Fu et al., 2019;Lin, 2019;Zhong et al., 2006). Plateau adaptability (PA) has always been the major direction of yak genome research.The different expressed genes (DEGs) or candidate quantitative trait loci (QTLs) related to low atmospheric pressure, hypoxia and high ultraviolet radiation in the plateau were used to veal underlying molecular mechanism in the resistance under high-altitude environ-

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Comparative expression profile of microRNAs and piRNAs in three ruminant species testes using next‐generation sequencing

Cited by 15 publications

References 47 publications

In silico identification of conserved miRNAs and their selective target gene prediction in indicine (Bos indicus) cattle

In silico identification of conserved miRNAs and their selective target gene prediction in indicine (Bos indicus) cattle

MiRNAs Expression Profiling of Bovine (Bos taurus) Testes and Effect of bta-miR-146b on Proliferation and Apoptosis in Bovine Male Germline Stem Cells

Comparative transcriptome analysis of winter yaks in plateau and plain

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