Comparative Expression Profiles of Midgut Genes in Dengue Virus Refractory and Susceptible Aedes aegypti across Critical Period for Virus Infection

Chauhan, Chitra; Behura, Susanta K.; deBruyn, Becky; Lovin, Diane D.; Harker, Brent W.; Gomez-Machorro, Consuelo; Mori, Akio; Romero‐Severson, Jeanne; Severson, David W.

doi:10.1371/journal.pone.0047350

Cited by 61 publications

(80 citation statements)

References 51 publications

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“…The contents, design of the custom cDNA microarray and the details of hybridization and scanning methods are described in Chauhan et al (2012). Briefly, microarrays were generated from 9,504 unique cDNA amplicons obtained from a various tissue sources to assist in the A. aegypti genome annotation effort (Nene et al 2007).…”

Section: Methodsmentioning

confidence: 99%

“…The data of the current study and a related study (Chauhan et al 2012) were compared to assess the role of vector genotype in transcriptional response to DENV infection. Both studies were carried out after infecting mosquitoes with same strain of virus (DENV-2 JAM1409) and the same refractory mosquito strain (Moyo-D).…”

Section: Methodsmentioning

confidence: 99%

“…Based on sequences of genes identified as responsive and non-responsive to the infection, it was found that different intrinsic features of A. aegypti genes are correlated with the transcriptional response to DENV infection (Behura and Severson 2012). Further, we observed that several responsive genes in a susceptible (Moyo-S) strain (Chauhan et al 2012) were also differentially expressed in another susceptible (Rockefeller) strain in response to dengue infection (Colpitt et al 2011). The primary goal of this study is to better understand the plasticity in transcriptome response to DENV infection among different A. aegypti genetic backgrounds.…”

Section: Introductionmentioning

confidence: 99%

“…However, the potential for differential response of these genes or pathways to DENV infection in refractory versus susceptible mosquito genotypes is unclear. In earlier studies, we employed microarrays to compare gene expression profiles in susceptible and refractory genotypes of A. aegypti and identified genes that are expressed in a highly networked manner to trigger a susceptible or refractory response to DENV infection (Behura et al 2011; Chauhan et al . 2012).…”

Section: Introductionmentioning

confidence: 99%

“…The primary goal of this study is to better understand the plasticity in transcriptome response to DENV infection among different A. aegypti genetic backgrounds. In an effort to achieve that objective, here we compare transcriptome profiles in the D2S3 (susceptible) and Moyo-D (refractory) strains of A. aegypti in response to DENV infection and compare results with that of Chauhan et al (2012) that identified gene expression patterns in Moyo-S (susceptible) vs. Moyo-D (refractory) strains. The results of this investigation show that a core set of genes are commonly differentially expressed in both D2S3/Moyo-D, as well as genes which are expressed in a susceptible or refractory strain-specific manner in response to DENV infection indicating that vector genotype plays an important role in conferring susceptible/refractory responses of the mosquito to dengue virus infection.…”

Section: Introductionmentioning

confidence: 99%

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Influence of mosquito genotype on transcriptional response to dengue virus infection

Behura

Gomez-Machorro

deBruyn

et al. 2014

Funct Integr Genomics

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The mosquito Aedes aegypti is the principal vector that transmits dengue virus (DENV) to humans. The primary factors that trigger a susceptible or refractory interaction of A. aegypti with DENV are not well understood. In this study, our aim is to characterize the influence of vector genotype on differential expression of susceptible vs. refractory A. aegypti strains to DENV infection. To accomplish that we identified differential expression of a set of cDNAs (n = 9,504) of the D2S3 (susceptible) and Moyo-D (refractory) strains of A. aegypti to dengue virus serotype 2 (JAM1409) and compared these results to the differential expression of cDNAs in a different susceptible vector genotype (Moyo-S) relative to the same refractory genotype (Moyo-D) identified from our previous study. We observed that, although the number of differentially expressed transcripts (DETs) were similar in both the studies, about ~95% of the DETs were distinct between Moyo-D/D2S3 vs. Moyo-D/Moyo-S. This suggested that A. aegypti response, to infection of a given genotype of dengue, is largely dependent upon the vector genotype. However, we observed a set of common DETs among the vector strains that were associated with predicted functions such as endocytosis, regulation of autophagy, peroxisome and lipid metabolism that may be relatively universal in conferring mosquito response to DENV infection.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Influence of mosquito genotype on transcriptional response to dengue virus infection

Behura

Gomez-Machorro

deBruyn

et al. 2014

Funct Integr Genomics

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Mosquito‐infecting virus Espirito Santo virus inhibits replication and spread of dengue virus

White

Ming

Mazzara

et al. 2020

Journal of Medical Virology

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The primary vector of dengue virus (DENV) is Aedes aegypti. The mosquito‐infecting virus, Espirito Santo virus (ESV), does not infect Vero (mammalian) cells and grows in mosquito (C6/36) cells without cytopathic effects. Effects of ESV infection on replication of DENV were explored in vitro and in vivo, analyzing protein, RNA genome expression, and plaque formation. ESV and DENV simultaneous coinfection did not block protein synthesis from either virus but did result in inhibition of DENV replication in mosquito cells. Furthermore, ESV superinfected with DENV resulted in inhibition of DENV replication and spread in A. aegypti, thus reducing vector competence. Tissue culture experiments on viral kinetics of ESV and DENV coinfection showed that neither virus significantly affects the replication of the other in Vero, HeLa, or HEK cells. Hence, ESV blocks DENV replication in insect cells, but not the mammalian cells evaluated here. Our study provides new insights into ESV‐induced suppression of DENV, a globally important pathogen impacting public health.

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Bicluster pattern of codon context usages between flavivirus and vector mosquito Aedes aegypti: relevance to infection and transcriptional response of mosquito genes

Behura

Severson

2014

Mol Genet Genomics

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The mosquito Aedes aegypti is the primary vector of dengue virus (DENV) infection in most of the subtropical and tropical countries. Besides DENV, yellow fever virus (YFV) is also transmitted by A. aegypti. Susceptibility of A. aegypti to West Nile virus (WNV) has also been confirmed. Although studies have indicated correlation of codon bias between flaviviridae and their animal/insect hosts, it is not clear if codon sequences have any relation to susceptibility of A. aegypti to DENV, YFV and WNV. In the current study, usages of codon context sequences (codon pairs for neighboring amino acids) of the vector (A. aegypti) genome as well as the flaviviral genomes are investigated. We used bioinformatics methods to quantify codon context bias in a genome-wide manner of A. aegypti as well as DENV, WNV and YFV sequences. Mutual information statistics was applied to perform bicluster analysis of codon context bias between vector and flaviviral sequences. Functional relevance of the bicluster pattern was inferred from published microarray data. Our study shows that codon context bias of DENV, WNV and YFV sequences varies in a bicluster manner with that of specific sets of genes of A. aegypti. Many of these mosquito genes are known to be differentially expressed in response to flaviviral infection suggesting that codon context sequences of A. aegypti and the flaviviruses may play a role in the susceptible interaction between flaviviruses and this mosquito. The bias inusages of codon context sequences likely has a functional association with susceptibility of A. aegypti to flaviviral infection. The results from this study will allow us to conduct hypothesis driven tests to examine the role of codon contexts bias in evolution of vector-virus interactions at the molecular level.

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Comparative Expression Profiles of Midgut Genes in Dengue Virus Refractory and Susceptible Aedes aegypti across Critical Period for Virus Infection

Cited by 61 publications

References 51 publications

Influence of mosquito genotype on transcriptional response to dengue virus infection

Influence of mosquito genotype on transcriptional response to dengue virus infection

Mosquito‐infecting virus Espirito Santo virus inhibits replication and spread of dengue virus

Bicluster pattern of codon context usages between flavivirus and vector mosquito Aedes aegypti: relevance to infection and transcriptional response of mosquito genes

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