Comparative Expression Profiling of Snf2 Family Genes During Reproductive Development and Stress Responses in Rice

Abstract

Sucrose non-fermenting 2 (Snf2) protein family, as chromatin remodeling factors, is an enormous and the most diverse protein family, which contributes to biological processes of replication, transcription, and DNA repair using the energy of adenosine triphosphate (ATP) hydrolysis. The members of Snf2 family proteins have been well characterized in Arabidopsis, rice, and tomato. Although this family received significant attention, few genes were identified uniquely for their roles in mediating reproductive development and stress tolerance in rice. In the present study, we comprehensively analyzed the expression profiling of Snf2 genes during reproductive development and biotic/abiotic stresses. Our results showed that five proteins (OsCHR712/715/720/726/739) were mainly localized in the nucleus, while OsCHR715/739 were also slightly expressed in the cell membrane. There were abundant cis-acting elements in the putative promoter of Snf2 genes, including dehydration, MeJA, MYB binding site for drought, ABA-responsive, and stress-responsive element. Most of the genes were induced immediately after Magnaporthe oryzae infection at 12 h post-infection (hpi). About 55% of the total genes were upregulated under salt and drought stresses during the entire time, and 22–35% of the total genes were upregulated at 3 h. It was noteworthy that the seven genes (OsCHR705, OsCHR706, OsCHR710, OsCHR714, OsCHR721, OsCHR726, and OsCHR737) were upregulated, and one gene (OsCHR712) was downregulated under salt and drought stresses, respectively. The deficiency of OsCHR726 mutations displayed a hypersensitive phenotype under salt stress. These results will be significantly useful features for the validation of the rice Snf2 genes and facilitate understanding of the genetic engineering of crops with improved biotic and abiotic stresses.

Linkage Mapping and Discovery of Candidate Genes for Drought Tolerance in Rice During the Vegetative Growth Period

“…Fu et al (2012) andChen et al (2023). In a previous study, qRT-PCR analysis of 14-day-old subjected to drought stress revealed that OsCHR731 was up-regulated after 12 h of treatment(Guo et al 2022), which is consistent with our ndings.Os04g0574600 encodes ae ZOS4-12-C2H2 zinc nger protein. C2H2 zinc nger proteins play an essential role in various developmental processes in plants, such as regulating plant growth, hormone signaling, and responses to biotic and abiotic stresses (Zhang et al 2016).…”

Guavacv.Allahabad Safeda Chromosome scale assembly and comparative genomics decodes breeders’ choice marker trait association for pink pulp colour

“…Among the stica, rsica, inella with clustered together, but also exhibited significant similarity to MYBs of other species. Among 23 TR classes, SNF2, playing roles in plant development, DNA repair and transcription regulation (Guo et al, 2022), was the most represented followed by PHD TR with role in histone binding, pathogen defense responses and plant development (Mouriz et al, 2015).…”

SNP Loci and Favorable Haplotype Mining for Alkalinity Tolerance in the Rice Bud Stage

“…At least six genes associated with cloned alkalinity tolerance in rice have been cloned [18][19][20][21][22][23][24][25]. OsLOL5 is a rice LSD1-like-type zinc finger protein (ZFP) gene containing two C2C2 domains, similar to LSD1.…”

“…ALT1 encodes the chromatin-remodeling ATPase of the Snf2 family. Studies on the alkali-resistant mutant alt1 in rice revealed that this mutant exhibits low hydrogen peroxide levels and tolerance to methyl purpurine treatment under alkalinity stress, which can reduce the production of ROS and play a positive role in alkalinity resistance by defending against oxidative damage to improve the alkalinity tolerance of rice [25]. OsSAP is a stress-associated protein that regulates soda saline-alkalinity tolerance through reactive oxygen species (ROS) homeostasis.…”