Comparative FISH mapping of bovine cosmids to reindeer chromosomes demonstrates conservation of the X-chromosome

Prakash, B.; Kuosku, V.; Olsaker, I.; Gustavsson, I.; Chowdhary, Bhanu P.

doi:10.1007/bf02254962

Cited by 9 publications

(6 citation statements)

References 15 publications

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“…For instance, Gallagher et al confirmed the conservation of X chromosome homology regions among members of Bovini, Boselaphini, Tragelaphini, domestic sheep and two deer species using BAC probes [ 27 ]. Comparative mapping of three X chromosome-specific bovine cosmids was employed in Rangifer tarandus gonosomes [ 33 ]. The results of another detailed study concerning conserved syntenies of the X chromosome in four Cervidae species are currently available [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

Karyotype relationships among selected deer species and cattle revealed by bovine FISH probes

et al. 2017

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The Cervidae family comprises more than fifty species divided into three subfamilies: Capreolinae, Cervinae and Hydropotinae. A characteristic attribute for the species included in this family is the great karyotype diversity, with the chromosomal numbers ranging from 2n = 6 observed in female Muntiacus muntjak vaginalis to 2n = 70 found in Mazama gouazoubira as a result of numerous Robertsonian and tandem fusions. This work reports chromosomal homologies between cattle (Bos taurus, 2n = 60) and nine cervid species using a combination of whole chromosome and region-specific paints and BAC clones derived from cattle. We show that despite the great diversity of karyotypes in the studied species, the number of conserved chromosomal segments detected by 29 cattle whole chromosome painting probes was 35 for all Cervidae samples. The detailed analysis of the X chromosomes revealed two different morphological types within Cervidae. The first one, present in the Capreolinae is a sub/metacentric X with the structure more similar to the bovine X. The second type found in Cervini and Muntiacini is an acrocentric X which shows rearrangements in the proximal part that have not yet been identified within Ruminantia. Moreover, we characterised four repetitive sequences organized in heterochromatic blocks on sex chromosomes of the reindeer (Rangifer tarandus). We show that these repeats gave no hybridization signals to the chromosomes of the closely related moose (Alces alces) and are therefore specific to the reindeer.

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Section: Introductionmentioning

confidence: 99%

Karyotype relationships among selected deer species and cattle revealed by bovine FISH probes

et al. 2017

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“…Cosmid probes were labeled with biotin by nick-transla tion using the BioNick kit according to the supplier's (Life Technologies) instructions. FISH was carried out as described previously (Scherthan et al, 1994, Prakash et al. 1996.…”

Section: Probes Labeling and Fishmentioning

confidence: 99%

“…To our knowl edge, only five genes have been assigned by blot hybridization to Indian muntjac chromosomes 2, X, Yh and Y2 (Shows et al, 1976;Levy et al, 1992). Recently, Prakash et al (1996) showed that bovine cosmid probes can serve as infraorder FISH map ping tools among Pecora species. Three bovine X-chromosome specific cosmids were comparatively mapped to the Rangifer tarandus X chromosome (Prakash et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, Prakash et al (1996) showed that bovine cosmid probes can serve as infraorder FISH map ping tools among Pecora species. Three bovine X-chromosome specific cosmids were comparatively mapped to the Rangifer tarandus X chromosome (Prakash et al, 1996). Extending this approach, we have obtained comparative mapping data among Muntiacus species and the cattle genome and report on the comparative assignment of five heterologous cattle cosmids to Indian and Chinese muntjac karyotypes.…”

Section: Introductionmentioning

confidence: 99%

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Segmental homology among cattle (Bos taurus), Indian muntjac (Muntiacus muntjak vaginalis), and Chinese muntjac (M. reevesi) karyotypes

Frönicke

Chowdhary²,

Scherthan³

1997

Cytogenet Genome Res

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In an attempt to examine homologies between Indian and Chinese muntjac karyotypes at a subchromosomal level, five bovine cosmids were comparatively mapped by heterologous fluorescence in situ hybridization (FISH). In the Indian muntjac (2n = 6) all cosmids mapped to chromosome 1, whereas in the Chinese muntjac (2n = 46) two cosmids mapped to chromosome 3 and one cosmid each mapped to chromosomes 1, 7, and 17. These markers have maintained their intrachromosomal position relative to a centromere/telomere axis in cattle and in Chinese and Indian muntjac chromosomal arms. Our results corroborate the tandem-fusion hypothesis for muntjac karyotypic evolution and establish orientational homology between the involved Chinese muntjac chromosomes and the discrete segments on Indian muntjac chromosome 1. Furthermore, our data disclose regional homologies between cattle and muntjac genomes and demonstrate the validity of intergeneric cosmid-FISH for investigations on karyotype evolution.

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“…This has been due to a great variety of evolutionary interspecific rearrangements, which include obvious changes in centromere placement, heterochromatic variation, and autosomal translocation. Additionally, there is evidence of intrachromosomal rearrangement of the X with interpretations ranging from subtle band differences (Buckland and Evans, 1978a) to multiple, and probably quite complex, chromosomal changes (Hayes et al, 1991;Gallagher et al, 1994;Prakash et al, 1996Prakash et al, , 1997Robinson et al, 1997).…”

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confidence: 99%

A molecular cytogenetic analysis of X chromosome repatterning in the Bovidae: transpositions, inversions, and phylogenetic inference

Robinson

Harrison

León

et al. 1998

Cytogenet Genome Res

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Chromosomal homologies among the X chromosomes of species representative of eight bovid subfamilies and most of the recognized tribes were established using a combination of FISH and conventional G- and C-banding. Our analyses allowed for the delimitation of three X chromosome types represented, respectively, by cattle (Bovinae, tribe Bovini), the tragelaphines (Bovinae, tribe Tragelaphini), and a large assemblage comprising all the remaining subfamilies and their tribes (the Cephalophinae, Hippotraginae, Alcelaphinae, Antilopinae, Aepycerotinae, Peleinae, and Caprinae). The use of the bacterial artificial chromosome probe BAC 101 (which maps to Xp12 in cattle) and an Xp painting probe comprising sequences specific for the short arm of cattle Xp (Xp24→p12) allowed us to orient this region, which has moved as a conserved euchromatic block during the evolution of the bovid X chromosome. We show that the differences between the three chromosomal types are attributable to a transposition, two inversions, and heterochromatic additions/deletions. A paucity of comparative mapping data precludes the assignment of the sequences contained in cattle Xp to either the presumed conserved (XCR) or the recently added (XAR) region of the eutherian X chromosome, and the reasons for the retention of these sequences as an evolutionarily conserved unit in the intrachromosomal restructuring of the bovid X across lineages remain enigmatic.

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Comparative FISH mapping of bovine cosmids to reindeer chromosomes demonstrates conservation of the X-chromosome

Cited by 9 publications

References 15 publications

Karyotype relationships among selected deer species and cattle revealed by bovine FISH probes

Karyotype relationships among selected deer species and cattle revealed by bovine FISH probes

Segmental homology among cattle (<i>Bos taurus</i>), Indian muntjac (<i>Muntiacus muntjak vaginali</i><i>s</i>), and Chinese muntjac (<i>M. reeves</i><i>i</i>) karyotypes

A molecular cytogenetic analysis of X chromosome repatterning in the Bovidae: transpositions, inversions, and phylogenetic inference

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