2019
DOI: 10.1002/biot.201900009
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Comparative Fluxome and Metabolome Analysis of Formate as an Auxiliary Substrate for Penicillin Production in Glucose‐Limited Cultivation of Penicillium chrysogenum

Abstract: During glucose-limited growth, a substantial input of adenosine triphosphate (ATP) is required for the production of β-lactams by the filamentous fungus Penicillium chrysogenum. Formate dehydrogenase has been confirmed in P. chrysogenum for formate oxidation allowing an extra supply of ATP, and coassimilation of glucose and formate has the potential to increase penicillin production and biomass yield. In this study, the steady-state metabolite levels and fluxes in response to cofeeding of formate as an auxilia… Show more

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Cited by 9 publications
(9 citation statements)
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“…An Agilent Zorbax SB-C 18 reversed-phase column (150 mm × 4.6 mm ID, 5 µm) was used to determine the concentrations of PAA, PenG, and byproducts in the penicillin biosynthetic pathway using equal gradient, reversed-phase high-performance liquid chromatography (HPLC). The mobile phase was 0.44 g/L potassium dihydrogen phosphate in acetonitrile/water (65/35, v/v); the injection volume was 5 µL, the detection wavelength was 214 nm, the flow rate was 1.5 mL/min and the column temperature was 25 • C [52].…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…An Agilent Zorbax SB-C 18 reversed-phase column (150 mm × 4.6 mm ID, 5 µm) was used to determine the concentrations of PAA, PenG, and byproducts in the penicillin biosynthetic pathway using equal gradient, reversed-phase high-performance liquid chromatography (HPLC). The mobile phase was 0.44 g/L potassium dihydrogen phosphate in acetonitrile/water (65/35, v/v); the injection volume was 5 µL, the detection wavelength was 214 nm, the flow rate was 1.5 mL/min and the column temperature was 25 • C [52].…”
Section: Methodsmentioning
confidence: 99%
“…For extracellular sampling, the cold steel-bead method, combined with liquid nitrogen, was used for the fast filtration and quenching of extracellular enzyme activities, as described previously [52]. For intracellular sampling, approximately 1.2 mL of broth was taken from the bioreactor into a tube containing the quenching solution (−27.5 • C, 40% v/v aqueous methanol).…”
Section: Rapid Sampling Quenching and Metabolite Extractionmentioning
confidence: 99%
“…For extracellular sampling, the cold steel-bead method combined with liquid nitrogen was used for the fast filtration and quenching of extracellular enzyme activities, as described previously [27]. For intracellular sampling, about 1 mL of broth was taken from the bioreactor into a tube containing the quenching solution (−27.5 • C, 40% V/V aqueous methanol).…”
Section: Rapid Sampling Quenching and Metabolite Extractionmentioning
confidence: 99%
“…The mobile phase consisted of 0.44 g KH 2 PO 4 per liter in the acetonitrile/water solution (65/35, V/V). The sample injection volume, the detection wave length, the flow rate and the column temperature were 5 µL, 214 nm, 1.5 mL/min and 25 • C, respectively [27].…”
Section: Analytical Proceduresmentioning
confidence: 99%
“…The functioning of the complex biological system can be understood by either top‐down (e.g., constraint‐based genome‐scale metabolic models) or bottom‐up approach (dynamic models based on differential equations; Kerkhoven, Lahtvee, & Nielsen, ). Targeted, quantitative metabolomics can yield significant insights into molecular mechanisms and metabolic regulation, which can be implemented to set up metabolic models describing intracellular activities (Cakir et al, ; Wang, Wang, et al, ). Strikingly, by combing metabolic flux analysis methods with other “omics” data sets (proteomics, transcriptomics), this new “omics” platform technology can help to provide a more complete understanding of a biological system (Buescher et al, ; Jang, Chen, & Rabinowitz, ; Matsuda et al, ; Toya & Shimizu, ; Wiechert & Noh, ; Zamboni, Saghatelian, & Patti, ).…”
Section: Quantitative Metabolomics and Its Application In Systems Metmentioning
confidence: 99%