Comparative Functional Analysis of Wheat (Triticum aestivum) Zinc Finger-Containing Glycine-Rich RNA-Binding Proteins in Response to Abiotic Stresses

Xu, Tao; Gu, Lili; Choi, Min Ji; Kim, Ryeo Jin; Suh, Mi Chung; Kang, Hunseung

doi:10.1371/journal.pone.0096877

Cited by 26 publications

(27 citation statements)

References 26 publications

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“…Because wheat transformation is technically difficult, we investigated TaRZ1 functions using transgenic Arabidopsis plants that express TaRZ1 under control of the cauliflower mosaic virus 35S promoter. Generation of multiple T 3 homozygote transgenic lines and confirmation of TaRZ1 expression in the transgenic lines were described in the previous report (Xu et al 2014). When the wild-type and transgenic Arabidopsis plants were grown in soil under normal conditions, all plants grew well with no noticeable differences until 2 weeks after germination.…”

Section: Wheat Tarz1 Inhibits Arabidopsis Growth and Reduces Seed Yiementioning

confidence: 98%

“…Seeds were harvested and plated on Murashige and Skoog (MS) medium containing hygromycin (50 lg ml -1 ) and carbenicillin (250 lg ml -1 ) to identify the transgenic plants. Detailed procedures for the generation of multiple T 3 homozygote transgenic lines and for the confirmation of TaRZ1 expression in the transgenic lines were described in the previous report (Xu et al 2014). …”

Section: Vector Construction and Generation Of Transgenic Arabidopsismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…A recent report demonstrated that TaRZs play negative roles in seed germination under salt or dehydration stress conditions and that seedling growth of TaRZ1-or TaRZ2-expressing transgenic Arabidopsis plants is inhibited under cold or salt stress conditions (Xu et al 2014). TaRZ2 was shown to possess RNA chaperone activity (Xu et al 2013) and to confer freeze tolerance in Arabidopsis plant (Xu et al 2014). To further determine the functional role of TaRZ1 in plant growth and biotic stress responses, transgenic Arabidopsis plants that express TaRZ1 under control of the cauliflower mosaic virus 35S promoter were generated, and growth of the transgenic plants was examined under normal conditions.…”

Section: Introductionmentioning

confidence: 99%

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Wheat (Triticum aestivum) zinc finger-containing glycine-rich RNA-binding protein TaRZ1 affects plant growth and defense response in Arabidopsis thaliana

Lee

et al. 2014

Plant Growth Regul

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Although the importance of zinc finger-containing glycine-rich RNA-binding proteins (RZs) during plant growth and stress adaptation has been determined in diverse plant species, the function and importance of RZs in wheat (Triticum aestivum) are largely unknown. Here, functional role of wheat TaRZ1 in the growth and defense response was evaluated using transgenic Arabidopsis thaliana. Ectopic expression of TaRZ1 in Arabidopsis resulted in retarded plant growth and reduced seed yield under normal growth conditions. Moreover, TaRZ1-expressing transgenic plants displayed increased resistance against the pathogenic bacteria Pseudomonas syringae DC3000. Microarray analysis showed that many genes related to plant growth, metabolism, and stress responses are differently up-or down-regulated in Arabidopsis by TaRZ1 expression. Taken together, these results reveal that wheat TaRZ1 has the ability to inhibit the growth of pathogenic bacteria and Arabidopsis, and suggest that TaRZ1 plays diverse roles in the growth and defense of plants.

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Section: Wheat Tarz1 Inhibits Arabidopsis Growth and Reduces Seed Yiementioning

confidence: 98%

Section: Vector Construction and Generation Of Transgenic Arabidopsismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Wheat (Triticum aestivum) zinc finger-containing glycine-rich RNA-binding protein TaRZ1 affects plant growth and defense response in Arabidopsis thaliana

Lee

et al. 2014

Plant Growth Regul

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“…These results suggested that the BrGRPs of class Ⅰ & class Ⅱ, Class Ⅳ & Class Ⅴ, each have the same ancestors which may develop additional conserved domain and get new functions during the evolution process. In addition, we also identified three Chinese cabbage Zinc Finger-ContainingGlycine-Rich RNA-Binding Proteins(RZs)[38] from the Class Ⅲ proteins, including Bra032933, Bra025250, Bra005798, all of which contained three single structural domains RRM, CSD, and CCHC, their function is still unknown.…”

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confidence: 99%

Genome-wide identification and expression analysis of glycine-rich protein genes in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

Gao

Cheng

et al. 2019

Preprint

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Background Plant Glycine-rich proteins, a superfamily with a glycine-rich domain, play an important role in various stress such as low temperature, drought, high salt, and so on. Although the research of GRP genes has been reported in many plants, the GRP gene has seldom reported in Chinese cabbage so far. Research results made a guide to further understand the function of BrGRP genes in Chinese cabbage. Results In this study, a total of 141 glycine-rich protein genes were identified in Chinese cabbage by homology comparative analysis. A further prediction of physical and chemical characteristics revealed that 58.3% of BrGRPs were alkalines, 63.1% of BrGRPs were unstable, and 73.8% were hydrophilic. Conserved domain analysis showed that 110 BrGRPs contained 18 same conserved motifs, and could be classified into five main subclasses which the evolutionary relationship and gene structure may be conserved while the other 31 BrGRPs, including Bra014168 , Bra040002 , etc, may gain new functions or gradually lost gene functions according to the evolution process. These identified BrGRP genes were also located in ten chromosomes and three different subgenomes of Chinese cabbage, and 101 pairs of orthologous GRP genes were found between Chinese cabbage and Arabidopsis. According to the opened transcriptome data, we found that 138 BrGRP genes showed abnormal expression at high temperature, 108 BrGRP genes showed abnormal expression at low temperature, and 74 at drought stress, 47 at soft rot stress, while only 3 and 7 genes at ozone and salt stress, respectively. Further promoter motif analysis found that a large number of stress-related cis-acting elements, such as DRE, MYC, MYB, and ABRE, were identified in their promoter regions, which were in correspondence with previous differential expression. In addition, some BrGRP genes were involved in multiple stresses suggested their broad-spectrum resistance. Conclusion A total of 141 GRP genes were identified in Chinese cabbage, which suggested their potential roles in plant stress response. But the molecular mechanisms by which BrGRP genes respond and resist biotic and abiotic stress remain unclear. These results may provide an important basis for the study of their function in Chinese cabbage.

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RNA chaperones: Potential candidates for engineering salt tolerance in rice

Ganie

2020

Crop Science

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RNA chaperone activity is crucial for proper folding and expression of messenger RNAs under abiotic stresses. There is functional conservation of plant and bacterial RNA chaperones, and they have been characterized mainly with respect to cold stress. The aim of this article was to compile all published rice (Oryza sativa L.) RNA chaperone genes, and to logically associate their RNA chaperone activity with salt tolerance. Based on the effects on transgenic plants under other abiotic stresses and the related published literature about the role of RNA chaperones in other plant species, I speculate that rice RNA chaperones are promising candidates for engineering salt tolerance in rice. Existing microarray data, showing that RNA chaperones of rice are highly responsive to salinity, are reviewed. Future functional studies will be required to characterize the range of functions of RNA chaperones in rice and other plants.Research avenues proposed in this review article provide a potential path to exciting research on rice salt tolerance.

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Comparative Functional Analysis of Wheat (Triticum aestivum) Zinc Finger-Containing Glycine-Rich RNA-Binding Proteins in Response to Abiotic Stresses

Cited by 26 publications

References 26 publications

Wheat (Triticum aestivum) zinc finger-containing glycine-rich RNA-binding protein TaRZ1 affects plant growth and defense response in Arabidopsis thaliana

Wheat (Triticum aestivum) zinc finger-containing glycine-rich RNA-binding protein TaRZ1 affects plant growth and defense response in Arabidopsis thaliana

Genome-wide identification and expression analysis of glycine-rich protein genes in Chinese cabbage (Brassica rapa L. ssp. pekinensis)

RNA chaperones: Potential candidates for engineering salt tolerance in rice

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