Comparative genome and transcriptome analysis reveals distinctive surface characteristics and unique physiological potentials of Pseudomonas aeruginosa ATCC 27853

Cao, Huiluo; Lai, Yong; Bougouffa, Salim; Xu, Zeling; Yan, Aixin

doi:10.1186/s12864-017-3842-z

Cited by 48 publications

(42 citation statements)

References 73 publications

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“…In the co‐culture of Pseudomonas sp #74 with P. aeruginosa ATCC 27853, the diameter of the halo was more than double in relation to the axenic culture ( anova , P < 0·05). In addition to a stimulation of the producer, this may indicate a contribution of the inducer to BSF release since P. aeruginosa ATCC 27853 has Las and Rhl quorum‐sensing genes mediating rhamnolipid synthesis (Pearson et al ; Cao et al ). This may also explain the large halos produced by co‐cultures of B. licheniformis with P. aeruginosa ATCC 27853, considering that with this test, BSF production was not detected in the corresponding axenic culture.…”

Section: Resultsmentioning

confidence: 99%

Increase in bacterial biosurfactant production by co‐cultivation with biofilm‐forming bacteria

Alves

Sequeira

2019

Lett Appl Microbiol

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Considering that bacterial biosurfactants (BSFs) are released as secondary metabolites involved in biotic relations within mixed bacterial assemblages, the hypothesis that the co‐cultivation of BSF producing bacteria with biofilm‐forming strains would enhance BSF synthesis was tested. Environmental BSF producing strains of Bacillus licheniformis and Pseudomonas sp. were cultivated with reference biofilm‐forming strains (Pseudomonas aeruginosa and Listeria innocua). BSF production and quorum‐quenching effects were tested in solid media. Tensioactive and anionic BSFs were also quantified in cell‐free extracts (CFEs). BSF production increased in co‐cultures with inducer strains although this was not demonstrated by all screening methods. Increased concentrations of anionic BSF were detected in CFEs of co‐cultures in which Pseudomonas aeruginosa was included as inducer, which is in accordance with the observation of larger halos in cetyl trimethylammonium bromide‐methylene blue agar. The results demonstrate that co‐cultivation positively affects the efficiency of BSF production and that higher production yields may be attained by selecting convenient inducer partners in designed consortia. Significance and Impact of the Study The high production cost of biosurfactants (BSFs) still represents a major limitation to the industrial use of these otherwise advantageous alternatives to chemical surfactants. This work demonstrates that the co‐cultivation of consortia of biosurfactant‐producer and biofilm‐forming bacteria enhances BSF production and may contribute to the cost‐effectiveness of biosurfactant‐based products.

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Section: Resultsmentioning

confidence: 99%

Increase in bacterial biosurfactant production by co‐cultivation with biofilm‐forming bacteria

Alves

Sequeira

2019

Lett Appl Microbiol

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“…Genomic DNA extraction. Extraction of the genomic DNA of PA154197 was performed following the description in a previous study (51). Briefly, PA154197 was cultivated in Luria-Bertani (LB) broth overnight with shaking (220 rpm) at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Uncoupled Quorum Sensing Modulates the Interplay of Virulence and Resistance in a Multidrug-Resistant Clinical Pseudomonas aeruginosa Isolate Belonging to the MLST550 Clonal Complex

Cao

Xia

et al. 2019

Antimicrob Agents Chemother

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Pseudomonas aeruginosa is a prevalent and pernicious pathogen equipped with extraordinary capabilities both to infect the host and to develop antimicrobial resistance (AMR). Monitoring the emergence of AMR high-risk clones and understanding the interplay of their pathogenicity and antibiotic resistance is of paramount importance to avoid resistance dissemination and to control P. aeruginosa infections. In this study, we report the identification of a multidrug-resistant (MDR) P. aeruginosa strain PA154197 isolated from a blood stream infection in Hong Kong. PA154197 belongs to a distinctive MLST550 clonal complex shared by two other international P. aeruginosa isolates VW0289 and AUS544. Comparative genome and transcriptome analysis of PA154197 with the reference strain PAO1 led to the identification of a variety of genetic variations in antibiotic resistance genes and the hyperexpression of three multidrug efflux pumps MexAB-OprM, MexEF-OprN, and MexGHI-OpmD in PA154197. Unexpectedly, the strain does not display a metabolic cost and a compromised virulence compared to PAO1. Characterizing its various physiological and virulence traits demonstrated that PA154197 produces a substantially higher level of the P. aeruginosa major virulence factor pyocyanin (PYO) than PAO1, but it produces a decreased level of pyoverdine and displays decreased biofilm formation compared with PAO1. Further analysis revealed that the secondary quorum-sensing (QS) system Pqs that primarily controls the PYO production is hyperactive in PA154197 independent of the master QS systems Las and Rhl. Together, these investigations disclose a unique, uncoupled QS mediated pathoadaptation mechanism in clinical P. aeruginosa which may account for the high pathogenic potentials and antibiotic resistance in the MDR isolate PA154197.

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“…Extraction of the genomic DNA of PA154197 was performed following the description in a previous study (44). Briefly, PA154197 was cultivated in Luria-Bertani (LB) broth overnight with shaking (220 rpm) at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…RNA extraction, quality control, and RNA-Seq were performed in PA154197 and the reference strain PAO1 with three biological replicates following our previous descriptions (44). Stranded libraries for all RNA samples were constructed using Kapa Biosystems RNA library preparation chemistry in Georgia Genomics Facility at University of Georgia.…”

Section: Methodsmentioning

confidence: 99%

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A multidrug resistant clinical P. aeruginosa isolate in the MLST550 clonal complex: uncoupled quorum sensing modulates the interplay of virulence and resistance

Cao¹,

Xia²,

Li³

et al. 2018

Preprint

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22Pseudomonas aeruginosa is a prevalent and pernicious pathogen equipped with both extraordinary 23 capabilities to infect the host and to develop antimicrobials resistance (AMR). Monitoring the 24 emergence of AMR high risk clones and understanding the interplay of their pathogenicity and 25 antibiotic resistance is of paramount importance to avoid resistance dissemination and to control 26 P. aeruginosa infections. In this study, we report the identification of a multidrug resistant (MDR) 27 P. aeruginosa strain PA154197 isolated from a blood stream infection in Hong Kong. PA154197 28 belongs to a distinctive MLST550 clonal complex shared by two international P. aeruginosa 29 isolates VW0289 and AUS544. Comparative genome and transcriptome analysis with the 30 reference strain PAO1 led to the identification of a variety of genetic variations in antibiotic 31 resistance genes and the hyper-expression of three multidrug efflux pumps MexAB-OprM, 32 MexEF-OprN, and MexGHI-OpmD in PA154197. Unlike many resistant isolates displaying an 33 attenuated virulence, PA154197 produces a significantly high level of the P. aeruginosa major 34 virulence factor pyocyanin (PYO) and displays an uncompromised virulence compared to PAO1. 35 Further analysis revealed that the secondary quorum sensing system Pqs which primarily controls 36 the PYO production is hyper-active in PA154197 independent of the master QS systems Las and 37 Rhl. Together, these investigations disclose a unique, uncoupled QS mediated pathoadaptation 38 mechanism in clinical P. aeruginosa which may account for the high pathogenic potentials and 39 antibiotics resistance in the MDR isolate PA154197. 40 41 Pseudomonas aeruginosa is a ubiquitous Gram-negative pathogen that causes a variety of 42 notorious infections in humans such as ventilator-associated pneumonia, lung infections of cystic 43 fibrosis (CF) patients, burn wound infection, and various sepsis syndromes. It is the second leading 44 cause of hospital-acquired infections and is especially problematic in ICUs, where it is the leading 45 cause of pneumonia among pediatric patients and is responsible for a large number of urinary tract 46 (10% in US and 19% in Europe), blood stream (3% in US and 10% in Europe), eye, ear, nose, and47 throat infections (1-3). Compounding the burden of these infections is the extraordinary capability 48 of the pathogen to develop antibiotic and multidrug resistance (MDR) even during the course of 49 antibiotics therapy. P. aeruginosa is one of the "ESKAPE" (Enterococcus spp., Staphylococcus 50 aureus, Klebsiella spp., Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter 51 spp.) organisms which are recognized by the WHO as an alarming threat to the global public heath 52 associated with antimicrobial resistance (AMR). As a consequence, the diseases outcome of the P. 53 aeruginosa infections is the complex interplay of the pathogen (its pathogenicity and virulence), 54 hospital environments (antibiotic therapies and the emergence of AMR), and the patie...

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Comparative genome and transcriptome analysis reveals distinctive surface characteristics and unique physiological potentials of Pseudomonas aeruginosa ATCC 27853

Cited by 48 publications

References 73 publications

Increase in bacterial biosurfactant production by co‐cultivation with biofilm‐forming bacteria

Increase in bacterial biosurfactant production by co‐cultivation with biofilm‐forming bacteria

Uncoupled Quorum Sensing Modulates the Interplay of Virulence and Resistance in a Multidrug-Resistant Clinical Pseudomonas aeruginosa Isolate Belonging to the MLST550 Clonal Complex

A multidrug resistant clinical P. aeruginosa isolate in the MLST550 clonal complex: uncoupled quorum sensing modulates the interplay of virulence and resistance

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