Comparative genome sequencing reveals insights into the dynamics of <i>Wolbachia</i> in native and invasive cherry fruit flies

Wolfe, Thomas M.; Bruzzese, Daniel J.; Klasson, Lisa; Corretto, Erika; Lečić, Sonja; Stauffer, Christian; Feder, Jeffrey L.; Schuler, Hannes

doi:10.1111/mec.15923

Cited by 19 publications

(17 citation statements)

References 137 publications

(191 reference statements)

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“…The w Di genome is largest among assembled Wolbachia genomes as compared with other Wolbachia from arthropods and nematodes. Previously, the largest Wolbachia genomes were from Folsomia candida (1.8 Mb) 19 , invasive cherry fruit fly Rhagoletis cingulata (1.53 Mb) 20 and embryos of Aedes albopictus (1.48 Mb) 25 .…”

Section: Resultsmentioning

confidence: 99%

“…In this study, we utilized HiCanu 18 for the complete assembly of genome sequences from w Di sample, which could resolve near-identical genomic repeats. The assembly resulted in a circular genome of 1.52 Mb which is the largest complete genome among assembled Wolbachia genomes to date among supergroup-B members, except for the complete Wolbachia genome from Folsomia candida ( w Fol) of 1.8 Mb 19 (supergroup-E), invasive cherry fruit fly Rhagoletis cingulata ( w Cin2) 20 of 1.53 Mb (supergroup-A). The genome dataset will enhance our ability to elucidate the interactions of w Di with its D. citri host and associated endosymbionts.…”

Section: Introductionmentioning

confidence: 99%

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Complete de novo assembly of Wolbachia endosymbiont of Diaphorina citri Kuwayama (Hemiptera: Liviidae) using long-read genome sequencing

Neupane

Bonilla

Manalo

et al. 2022

Sci Rep

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Wolbachia, a gram-negative $$\mathrm{\alpha }$$ α -proteobacterium, is an endosymbiont found in some arthropods and nematodes. Diaphorina citri Kuwayama, the vector of ‘Candidatus Liberibacter asiaticus’ (CLas), are naturally infected with a strain of Wolbachia (wDi), which has been shown to colocalize with the bacteria pathogens CLas, the pathogen associated with huanglongbing (HLB) disease of citrus. The relationship between wDi and CLas is poorly understood in part because the complete genome of wDi has not been available. Using high-quality long-read PacBio circular consensus sequences, we present the largest complete circular wDi genome among supergroup-B members. The assembled circular chromosome is 1.52 megabases with 95.7% genome completeness with contamination of 1.45%, as assessed by checkM. We identified Insertion Sequences (ISs) and prophage genes scattered throughout the genomes. The proteins were annotated using Pfam, eggNOG, and COG that assigned unique domains and functions. The wDi genome was compared with previously sequenced Wolbachia genomes using pangenome and phylogenetic analyses. The availability of a complete circular chromosome of wDi will facilitate understanding of its role within the insect vector, which may assist in developing tools for disease management. This information also provides a baseline for understanding phylogenetic relationships among Wolbachia of other insect vectors.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Complete de novo assembly of Wolbachia endosymbiont of Diaphorina citri Kuwayama (Hemiptera: Liviidae) using long-read genome sequencing

Neupane

Bonilla

Manalo

et al. 2022

Sci Rep

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“…w AlbB diversity has commonly been investigated using a limited number of markers such as the wsp gene and Multi-Locus Sequence Typing (MLST) genes with little to no variation observed between isolates from different locations (42–44). Whole-genome sequencing provides a higher resolution and has revealed significant genomic differences between closely-related Wolbachia strains (45–47). Using this method, we demonstrated the existence of at least two types of w AlbB variants that differ in genome synteny and gene content.…”

Section: Discussionmentioning

confidence: 99%

Genomic and phenotypic comparisons reveal distinct variants of Wolbachia strain wAlbB

Martinez¹,

Ross²,

Gu³

et al. 2022

Preprint

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The intracellular bacterium Wolbachia inhibits virus replication and is being harnessed around the world to fight mosquito-borne diseases through releases of mosquitoes carrying the symbiont. Wolbachia strains vary in their ability to invade mosquito populations and suppress viruses in part due to differences in their density within the insect and associated fitness costs. Using whole-genome sequencing, we demonstrate the existence of two variants in wAlbB, a Wolbachia strain being released in natural populations of Aedes aegypti mosquitoes. The two variants display striking differences in genome architecture and gene content. Differences in the presence/absence of 49 genes between variants include genes located in prophage regions and others potentially involved in controlling the symbiont’s density. Importantly, we show that these genetic differences correlate with variation in wAlbB density and its tolerance to heat stress, suggesting that different wAlbB variants may be better suited for field deployment depending on local environmental conditions. Finally, we found that the wAlbB genome remained stable following its introduction in a Malaysian mosquito population. Our results highlight the need for further genomic and phenotypic characterization of Wolbachia strains in order to inform ongoing Wolbachia-based programmes and improve the selection of optimal strains in future field interventions.

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“…We believe that further studies should focus in specific groups of ants to help elucidate the complex history and evolutionary relationship of Wolbachia with ants. Moreover, given the recent studies on Wolbachia genome and the low resolution on MLST approach [24,25], new approaches, such as comparative genome studies, should change substantially our knowledge of this complex history and help understand the real strain diversity and the evolutionary dynamics of Wolbachia and its host.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, to our knowledge there is no further investigations regarding Wolbachia diversity with a focus exclusively on native Solenopsis using MLST approach, which highlights the need to deepen studies with a more precise methodology to reconstruct the evolution and review the diversity of Wolbachia strains in Solenopsis species. Although genomic approches are more robust and suitable to undertand Wolbachia dynamics and the real strain diversity (see References [24][25][26]), the difficulty of accessing many genomes due to the high costs makes MLST a more accessible method for initial surveys of specific groups of host and larger samples. Furthermore, the number of Wolbachia ant genomes are still restricted [26,27], and the MLST has an extensive database for initial comparative analyses.…”

Section: Introductionmentioning

confidence: 99%

New Strains of Wolbachia Unveiling the Complexity of This Symbiotic Interaction in Solenopsis (Hymenoptera: Formicidae)

Martins¹,

Ramalho

Silva

et al. 2021

Microbiology Research

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Bacteria of the genus Wolbachia are widely distributed in arthropods, particularly in ants; nevertheless, it is still little explored with the Multilocus Sequence Typing (MLST) methodology, especially in the genus Solenopsis, which includes species native to South America. Ants from this genus have species distributed in a cosmopolitan way with some of them being native to South America. In Brazil, they are widely spread and preferentially associated with areas of human activity. This study aimed to investigate the diversity of Wolbachia in ants of the genus Solenopsis through the MLST approach and their phylogenetic relationship, including the relationship between mtDNA from the host and the related Wolbachia strain. We also tested the geographic correlation between the strains to infer transmission and distributional patterns. Fifteen new strains and eleven previously unknown alleles were obtained by sequencing and analyzing the five genes that make up the MLST. The phylogenetic relationship between the strains showed a polyphyletic pattern, indicative of the complexity of the evolutionary history of these bacteria in the analyzed species. We detected the correlation of host’s mitochondrial DNA with Wolbachia diversity which imply that related strains exist in related hosts, strongly suggesting the occurrence of vertical transfer. We found no specificity of the Wolbachia strain for a given geographic region that could indicate either that there is no horizontal transfer of the strain from the environment for the host or that the human action could be shuffling the distribution of the Solenopsis ants and the endosymbiont Wolbachia, as well. Our study highlights the complexity and novelty of Wolbachia diversity with this specific group of ants and the need for further studies that focus on understanding of this intricate relationship.

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Comparative genome sequencing reveals insights into the dynamics of Wolbachia in native and invasive cherry fruit flies

Cited by 19 publications

References 137 publications

Complete de novo assembly of Wolbachia endosymbiont of Diaphorina citri Kuwayama (Hemiptera: Liviidae) using long-read genome sequencing

Complete de novo assembly of Wolbachia endosymbiont of Diaphorina citri Kuwayama (Hemiptera: Liviidae) using long-read genome sequencing

Genomic and phenotypic comparisons reveal distinct variants of Wolbachia strain wAlbB

New Strains of Wolbachia Unveiling the Complexity of This Symbiotic Interaction in Solenopsis (Hymenoptera: Formicidae)

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