Comparative genomic analyses of<i>Mycoplasma synoviae</i>vaccine strain MS-H and its wild-type parent strain 86079/7NS: implications for the identification of virulence factors and applications in diagnosis of<i>M. synoviae</i>

Zhu, Ling; Shahid, Muhammad Akbar; Markham, John; Browning, Glenn F.; Noormohammadi, Amir H.; Marenda, Marc S.

doi:10.1080/03079457.2019.1637514

Cited by 15 publications

(21 citation statements)

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“…1). Table 1 Nucleotide and coding differences identified among the genomes of 86,079/7NS, MS-H and 5 MS-H field isolates Nucleotide position a 86079/7NS MS-H b MS-H 3 b MS-H 4 b MS-H 5 b 101546 b 101731 b Protein/region Functional category Amino acid change G A A A A A A tRNA (Guanine37-N1) methyltransferase tRNA metabolism Val→Ile hydrophobic to hydrophobic alkyl to alkyl A T T T T T T Non-coding region G A A A A A A DNA T T T A T T T Hypothetical protein Unknown Asp→Val hydrophilic to hydrophobic acidic to alkyl G A A A A A A P80-related protein Unknown Pro→Ser hydrophobic to hydrophilic alkyl to neutral C A A A A A A Non-coding region G A A A A A A NAD-dependent glyceraldehyde -3-phosphate dehydrogenase Glucose metabolism Ala→Val hydrophobic to hydrophobic alkyl to alkyl G A A A A G A NAD-dependent glyceraldehyde -3-phosphate dehydrogenase Glucose metabolism Arg→Lys hydrophilic to hydrophilic basic to basic T T T T T [12], however the other 21 were found only in the 5 reisolates.…”

Section: Resultsmentioning

confidence: 99%

“…Partial sequence analysis of obg and oppF genes [8,12] in five MS-H isolates have found different combinations of obg and oppF genotypes. Of the five isolates, MS-H 3 , 101,564 and 101,731 had obg w (w = wild-type) and oppF v (v = vaccine-type), MS-H 4 had obg w and oppF w , and MS-H 5 had obg v and oppF w .…”

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confidence: 99%

“…Of the five isolates, MS-H 3 , 101,564 and 101,731 had obg w (w = wild-type) and oppF v (v = vaccine-type), MS-H 4 had obg w and oppF w , and MS-H 5 had obg v and oppF w . In this study the MS-H reisolates MS-H 3 , 101,564, 101, 731 MS-H 4 , and MS-H 5 were subjected to a comparative genome analysis to establish if any other mutations previously reported for the MS-H [12] may be reversible to the wild-type and evaluate if they could potentially influence MS-H attenuation.…”

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confidence: 99%

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Preliminary comparative analysis of the genomes of selected field reisolates of the Mycoplasma synoviae vaccine strain MS-H reveals both stable and unstable mutations after passage in vivo

et al. 2020

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Background Genomic comparison of Mycoplasma synoviae vaccine strain MS-H and the MS-H parental strain 86,079/7NS established a preliminary profile of genes related to attenuation of MS-H. In this study we aimed to identify the stability of mutations found in MS-H after passage in experimental or field chickens, and to evaluate if any reverse mutation may be associated with changes in characteristics of MS-H in vitro or in vivo. Results Whole genome sequence analysis of 5 selected MS-H field reisolates revealed that out of 32 mutations reported previously in MS-H, 28 remained stable, while four found to be reversible to the wild-type. Each isolate possessed mutations in one to three of the genes obg, oppF1 and gap and/or a non-coding region. Examination of the 4 reversible mutations by protein modeling predicted that only two of them (in obg and oppF1 genes) could potentially restore the function of the respective protein to that of the wild-type. Conclusions These results suggest that the majority of the MS-H mutations are stable after passage in vaccinated chickens. Characterisation of stable mutations found in MS-H could be utilised to develop rapid diagnostic techniques for differentiation of vaccine from field strains or ts- MS-H reisolates.

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Preliminary comparative analysis of the genomes of selected field reisolates of the Mycoplasma synoviae vaccine strain MS-H reveals both stable and unstable mutations after passage in vivo

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“…Interference with such transporters is likely to affect the growth and viability of mycoplasmas substantially, so essential transporters are likely to be promising targets in future rationale vaccine efforts. Candidates have already been identified in other Mycoplasma species that are present in the "Mycoplasma mycoides cluster", including the putative oligopeptide/dipeptide (opp/dpp) ATP-binding cassette (ABC) transporter 66,67 . Therefore, a combination of in silico analysis and OMICS data (proteomics, transcriptomics, metabolomics) is likely to enable us to identify transporters that should be target for future characterization.…”

Section: Candidate Vaccine Antigens and Attenuation Targets -Filling mentioning

confidence: 99%

“…npj Vaccines (2020)66 Published in partnership with the Sealy Center for Vaccine Development1234567890():,;…”

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Contagious Bovine and Caprine Pleuropneumonia: a research community’s recommendations for the development of better vaccines

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Contagious bovine pleuropneumonia (CBPP) and contagious caprine pleuropneumonia (CCPP) are major infectious diseases of ruminants caused by mycoplasmas in Africa and Asia. In contrast with the limited pathology in the respiratory tract of humans infected with mycoplasmas, CBPP and CCPP are devastating diseases associated with high morbidity and mortality. Beyond their obvious impact on animal health, CBPP and CCPP negatively impact the livelihood and wellbeing of a substantial proportion of livestock-dependent people affecting their culture, economy, trade and nutrition. The causative agents of CBPP and CCPP are Mycoplasma mycoides subspecies mycoides and Mycoplasma capricolum subspecies capripneumoniae, respectively, which have been eradicated in most of the developed world. The current vaccines used for disease control consist of a live attenuated CBPP vaccine and a bacterin vaccine for CCPP, which were developed in the 1960s and 1980s, respectively. Both of these vaccines have many limitations, so better vaccines are urgently needed to improve disease control. In this article the research community prioritized biomedical research needs related to challenge models, rational vaccine design and protective immune responses. Therefore, we scrutinized the current vaccines as well as the challenge-, pathogenicity-and immunity models. We highlight research gaps and provide recommendations towards developing safer and more efficacious vaccines against CBPP and CCPP.

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Mycoplasmas

Shil¹,

Wawegama²,

Browning³

et al. 2022

Pathogenesis of Bacterial Infections in Animals

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Comparative genomic analyses ofMycoplasma synoviaevaccine strain MS-H and its wild-type parent strain 86079/7NS: implications for the identification of virulence factors and applications in diagnosis ofM. synoviae

Cited by 15 publications

References 90 publications

Preliminary comparative analysis of the genomes of selected field reisolates of the Mycoplasma synoviae vaccine strain MS-H reveals both stable and unstable mutations after passage in vivo

Preliminary comparative analysis of the genomes of selected field reisolates of the Mycoplasma synoviae vaccine strain MS-H reveals both stable and unstable mutations after passage in vivo

Contagious Bovine and Caprine Pleuropneumonia: a research community’s recommendations for the development of better vaccines

Mycoplasmas

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