Comparative genomic analysis and proposal of Clostridium yunnanense sp. nov., Clostridium rhizosphaerae sp. nov., and Clostridium paridis sp. nov., three novel Clostridium sensu stricto endophytes with diverse capabilities of acetic acid and ethanol production

Li, Cong-Jian; Zhang, Zhen; Zhan, Peng-Chao; Ai-ping, LV; Li, Pan-Pan; Liu, Lan; Li, Wenjun; Yang, Ling–Ling; Zhi, Xiao‐Yang

doi:10.1016/j.anaerobe.2022.102686

Cited by 26 publications

(11 citation statements)

References 69 publications

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“…Notably, in this study, Clostridium sensu stricto 1 showed a positive correlation with muscle mass. Interestingly, Clostridium sensu stricto 1, identified as a producer of short-chain fatty acids (SCFAs), aligns with research demonstrating the muscle-protective benefits of dietary SCFA intake in older adults [59,60]. Butyrate, one of the representative SCFAs, promotes C2C12 myoblast proliferation [61] and alleviates muscle atrophy by enhancing gut barrier function and activating the PI3K/Akt/mTOR signaling cascade [62].…”

Section: Discussionmentioning

confidence: 64%

Cornflower Extract and Its Active Components Alleviate Dexamethasone-Induced Muscle Wasting by Targeting Cannabinoid Receptors and Modulating Gut Microbiota

Nguyen,

Le,

Kang

et al. 2024

Nutrients

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Sarcopenia, a decline in muscle mass and strength, can be triggered by aging or medications like glucocorticoids. This study investigated cornflower (Centaurea cyanus) water extract (CC) as a potential protective agent against DEX-induced muscle wasting in vitro and in vivo. CC and its isolated compounds mitigated oxidative stress, promoted myofiber growth, and boosted ATP production in C2C12 myotubes. Mechanistically, CC reduced protein degradation markers, increased mitochondrial content, and activated protein synthesis signaling. Docking analysis suggested cannabinoid receptors (CB) 1 and 2 as potential targets of CC compounds. Specifically, graveobioside A from CC inhibited CB1 and upregulated CB2, subsequently stimulating protein synthesis and suppressing degradation. In vivo, CC treatment attenuated DEX-induced muscle wasting, as evidenced by enhanced grip strength, exercise performance, and modulation of muscle gene expression related to differentiation, protein turnover, and exercise performance. Moreover, CC enriched gut microbial diversity, and the abundance of Clostridium sensu stricto 1 positively correlated with muscle mass. These findings suggest a multifaceted mode of action for CC: (1) direct modulation of the muscle cannabinoid receptor system favoring anabolic processes and (2) indirect modulation of muscle health through the gut microbiome. Overall, CC presents a promising therapeutic strategy for preventing and treating muscle atrophy.

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Section: Discussionmentioning

confidence: 64%

Cornflower Extract and Its Active Components Alleviate Dexamethasone-Induced Muscle Wasting by Targeting Cannabinoid Receptors and Modulating Gut Microbiota

Nguyen,

Le,

Kang

et al. 2024

Nutrients

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“…Their presence in the microbial community was in line with the accumulation of caproate, heptanoate, and caprylate in the fermentate. The presence of Clostridium sensu stricto 7 after adaptive evolution was interesting because related taxa possess the genetic basis involved in butanol production 31 . Unidentified members of Ruminococcaceae could be involved in the hydrolysis of polysaccharides to soluble sugars, and then Caproiciproducens and Clostridium sensu stricto 7 fermented the released sugars to short‐chain fatty acids and solvents, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…The presence of Clostridium sensu stricto 7 after adaptive evolution was interesting because related taxa possess the genetic basis involved in butanol production. 31 Unidentified members of Ruminococcaceae could be involved in the hydrolysis of polysaccharides to soluble sugars, and then Caproiciproducens and Clostridium sensu stricto 7 fermented the released sugars to short-chain fatty acids and solvents, respectively. In subcommunity II, the fermentative taxa Caproiciproducens and Anaerofilum, and the fibrolytic Bacteroides were the key nodes.…”

Section: Co-occurrence Networkmentioning

confidence: 99%

Butanol and caproate production by consolidated bioprocessing after adaptive evolution of a fermentative microbial community

González-Tenorio

Dudek

Valdez‐Vazquez

2023

Biofuels Bioprod Bioref

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Butanol production from cellulose by consolidated bioprocessing may accelerate the commercialization of lignocellulosic biofuels. This study applied adaptive evolution to a natural microbial community consisting of different taxa of fermenters and plant‐degrading bacteria to increase its production of butanol. To this end, the parental microbial community was subjected to the stepwise enrichment of butanol at four initial concentrations in repeated‐batch fermentations using pretreated corn stover as the sole substrate. After the adaptive evolution, the microbial community exposed to the highest concentration of butanol produced 13.8 g L‐1 butanol, which was nine times higher than the parental microbial community. The adapted microbial community also produced the medium‐chain fatty acid caproate (7.5 g L‐1) from the condensation of alcohols (ethanol/butanol) with short‐chain fatty acids at the end of the fermentation. A network analysis revealed that the largest subcommunities were represented by Clostridium sensu stricto 7 and Caproiciproducens, which are associated with the production of alcohols (ethanol/butanol) and medium‐chain fatty acids, respectively. In the smaller subcommunities, Pseudomonas, Sutterella, and Pandoraea became more important after adaptive evolution, suggesting the production of extracellular polymeric substances and cross‐stress protection as mechanisms of protection and adaptation against butanol. This study demonstrated that butanol production by the consolidated bioprocessing of cellulose required solventogenic bacteria, diverse taxa of plant‐degrading bacteria that established unique interactions with the fermenters and, more important, members with mechanisms of protection and tolerance to solvents.

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“…This genus describes strictly anaerobic fermenting spore-formers [51]. During the metabolisation of sugars and proteins, the main fermentation products are butyric and acetic acid [52]. The genus is also able to produce lactic acid and ethanol, propanol or butanol.…”

Section: Discussionmentioning

confidence: 99%

Microwave assisted acidification for green hydrogen and organic acids production

Barth,

Werner,

Otto

et al. 2023

Preprint

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Heat shocks are a favoured method used to trigger hydrogen production in dark fermentation. However, these treatments are often restricted to a very small volume, which is then inoculated into synthetic medium. Moreover, heat shocks are usually applied with an oven. This study explored the feasibility of applying heat shocks to the entire seed sludge using a microwave, a method not previously investigated. The experiments were conducted on a technical scale with 5 litre tank reactors. The highest productivity was achieved with an oven (0.95 mol H2/mol hexose), but the microwave was not significantly different and had the advantage of heating the sludge 6.4 times faster. The study also emphasized the repeatability of heat shocks, with microwave-assisted experiments showing low variation coefficients averaging 29%. The approach is already economically sustainable with high-temperature heat pumps with a COP of 4.3, enhancing its practical relevance.Highlights-The entire reactor content of 3.5 litre was heat shocked.-Heat shocks economically boosted dark fermentation hydrogen yield.-Heat pumps with COP of 4.3 would allow energetic net gain due to heat shocks.-Oven and microwave-based heat shocks are not significantly different.-Microwaves heat sludge 6.4 times faster than ovens.-Heat shocks inhibited methane production completely.-Heat shocks increased particularlyClostridium sensu stricto1 and 11.-Hydrogen could be enriched from sucrose, but not from lipids or proteins.

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Comparative genomic analysis and proposal of Clostridium yunnanense sp. nov., Clostridium rhizosphaerae sp. nov., and Clostridium paridis sp. nov., three novel Clostridium sensu stricto endophytes with diverse capabilities of acetic acid and ethanol production

Cited by 26 publications

References 69 publications

Cornflower Extract and Its Active Components Alleviate Dexamethasone-Induced Muscle Wasting by Targeting Cannabinoid Receptors and Modulating Gut Microbiota

Cornflower Extract and Its Active Components Alleviate Dexamethasone-Induced Muscle Wasting by Targeting Cannabinoid Receptors and Modulating Gut Microbiota

Butanol and caproate production by consolidated bioprocessing after adaptive evolution of a fermentative microbial community

Microwave assisted acidification for green hydrogen and organic acids production

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