Comparative Genomic Analysis for Genetic Variation in Sacbrood Virus of Apis cerana and Apis mellifera Honeybees From Different Regions of Vietnam

Reddy, Kondreddy Eswar; Thu, Hà Thị; Yoo, Mi Sun; Ramya, Mummadireddy; Reddy, Bheemireddy Anjana; Liên, Nguyễn Thị Kim; Trang, Nguyen Thi; Lee, Hyun-Jeong; Kang, Sang Wook; Quyền, Đồng Văn

doi:10.1093/jisesa/iex077

Cited by 10 publications

(19 citation statements)

References 35 publications

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“…Similar to AmCSBV-SDLY-2016, two Vietnamese SBVs strains (AmSBV-Vt-4-2014 and AmSBV-Viet6-2014) and two Korean strains (AmSBV-Kor19-2012 and AmSBV-Kor2-2016) isolated from A. mellifera also belong to the AC type (Fig. 6), consistent with previous reports (Reddy et al, 2017; Choe et al, 2012b). Although no reports are available to determine if this strain caused A. mellifera larval deaths, the results of the present study show that the long-term existence and widespread prevalence of the AC type SBV in nature may lead to exchange of viruses among host populations.…”

Section: Discussionsupporting

confidence: 90%

“…The differences between the AC and AM genotypes may result from the adaptation of the virus to different hosts and the existence of different subgroups of the AC genotype based on regional variations (Choe et al, 2012a; Grabensteiner et al, 2001; Ma et al, 2013b). The AC genotype SBV strains were mainly isolated from Asian countries, and their hosts include A. cerana (Zhang et al, 2001; Zhang, 2012; Reddy et al, 2016, 2017; Ma et al, 2011c; Nguyen & Le, 2013; Choe et al, 2012b; Xia, Zhou & Wei, 2015; Hu et al, 2016; Yu, Liu & Wang, 2016). However, the AM genotype SBV persists in the bee colony and may cause infection of A. mellifera (Allen & Ball, 1996; Berenyi et al, 2006; Ellis & Munn, 2005; Cavigli et al, 2016; Tsevegmid, Neumann & Yañez, 2016; Desai & Currie, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…The genetic characterization and phylogenetic relationship of SBV-infected honeybees collected from different hosts and various geographic regions have recently attracted attention. Previous studies have focused on the alignment, basic structure, and composition of SBV/CSBV genomes, and host-specificity and geographic differences of SBV/CSBV (Choe et al, 2012a, 2012b; Grabensteiner et al, 2001; Reddy et al, 2016, 2017; Ma et al, 2011c, 2013b; Nguyen & Le, 2013; Xia, Zhou & Wei, 2015; Hu et al, 2016; Yu, Liu & Wang, 2016; Zhang et al, 2001); however, it is unclear whether there is recombination between SBV and CSBV and if CSBV breaks through the species barrier, develops cross-infection, and causes disease (kills larvae) in A. mellifera . Using artificial infection experiments, Gong et al (2016) demonstrated that CSBV is able to infect A. mellifera , but did not observe obvious signs of the disease, which indicated low pathogenicity.…”

Section: Introductionmentioning

confidence: 99%

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Genetic and phylogenetic analysis of Chinese sacbrood virus isolates fromApis mellifera

Li¹,

Fei²,

Sun³

et al. 2019

PeerJ

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BackgroundSacbrood virus (SBV) is one of the most pathogenic honeybee viruses that exhibits host specificity and regional variations. The SBV strains that infect the Chinese honeybee Apis cerana are called Chinese SBVs (CSBVs).MethodsIn this study, a CSBV strain named AmCSBV-SDLY-2016 (GenBank accession No. ) infecting A. mellifera was identified by electron microscopy, its protein composition was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and agar gel immunodiffusion assay, and its nucleotide sequence was identified using a series of reverse-transcription polymerase chain reaction fragments of AmCSBV-SDLY-2016 generated using SBV/CSBV-specific primers. To investigate phylogenetic relationships of the CSBV isolates, a phylogenetic tree of the complete open reading frames (ORF) of the CSBV sequences was constructed using MEGA 6.0; then, the similarity and recombination events among the isolated CSBV strains were analyzed using SimPlot and RDP4 software, respectively.ResultsSequencing results revealed the complete 8,794-nucleotide long complete genomic RNA of the strain, with a single large ORF (189–8,717) encoding 2,843 amino acids. Comparison of the deduced amino acid sequence with the SBV/CSBV reference sequences deposited in the GenBank database identified helicase, protease, and RNA-dependent RNA polymerase domains; the structural genes were located at the 5′ end, whereas the non-structural genes were found at the 3′ end. Multiple sequence alignment showed that AmCSBV-SDLY-2016 had a 17-amino acid (aa) and a single aa deletion at positions 711–729 and 2,128, respectively, as compared with CSBV-GD-2002, and a 16-aa deletion (positions 711–713 and 715–728) as compared with AmSBV-UK-2000. However, AmCSBV-SDLY-2016 was similar to the CSBV-JLCBS-2014 strain, which infects A. cerana. AmCSBV-SDLY-2016 ORF shared 92.4–97.1% identity with the genomes of other CSBV strains (94.5–97.7% identity for deduced amino acids). AmCSBV-SDLY-2016 was least similar (89.5–90.4% identity) to other SBVs but showed maximum similarity with the previously reported CSBV-FZ-2014 strain. The phylogenetic tree constructed from AmCSBV-SDLY-2016 and 43 previously reported SBV/CSBV sequences indicated that SBV/CSBV strains clustered according to the host species and country of origin; AmCSBV-SDLY-2016 clustered with other previously reported Chinese and Asian strains (AC genotype SBV, as these strains originated from A. cerana) but was separate from the SBV genomes originating from Europe (AM genotype SBV, originating from A. mellifera). A SimPlot graph of SBV genomes confirmed the high variability, especially between the AC genotype SBV and AM genotype SBV. This genomic diversity may reflect the adaptation of SBV to specific hosts, ability of CSBV to cross the species barrier, and the spatial distances that separate CSBVs from other SBVs.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic and phylogenetic analysis of Chinese sacbrood virus isolates fromApis mellifera

Li¹,

Fei²,

Sun³

et al. 2019

PeerJ

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“…CSBV and SBV have similar genomes and sizes (26–30 nm in diameter), but they differ in their physiochemical characteristics, immunogenicity and pathogenicity in honeybees (Hu et al., ). CSBV belongs to the Picornavirales (order), Iflaviridae (family), and Iflavirus (genus) order, which represent a group of positive‐sense single‐stranded RNA viruses (Ahn et al., ; Hu et al., ; Reddy et al., ; Roy, Vidal‐Naquet, & Provost, ; Shan et al., ; Xia et al., ; Yoo et al., ). SBV infects mostly larvae, including adult bees, because the adult bees do not present disease symptoms and there is a failure of infection in pupae (Ahn et al., ; Choe, Nguyen, Hyun et al., ; Choe, Nguyen, Jin et al., ; Roy et al., ; Xia et al., ; Zhang et al., ).…”

Section: Discussionmentioning

confidence: 99%

Prevalence of bee viruses in Apis cerana cerana populations from different locations in the Fujian Province of China

Hassanyar

Huang

et al. 2019

MicrobiologyOpen

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Prevalence of honeybee viral diseases has recently been causing major problems in the beekeeping industry, causing economic losses worldwide. Honeybees are susceptible to a variety of diseases and various pathogens. Among these pathogens, prevalence viruses, along with other factors, are seriously threatening the health of bee species. In the present study, samples were collected from 80 Apis cerana cerana (A. c. cerana) colonies from three different locations, Cangshan, Fuan, and Yongtai, in the Fujian Province of China. All samples were screened using the reverse transcription polymerase chain reaction (RT‐PCR) method for detection of seven honeybee viruses, namely, Chinese sacbrood virus (CSBV), deformed wing virus (DWV), Israeli acute paralysis virus (IAPV), black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), acute bee paralysis virus (ABPV), and Kashmir bee virus (KBV). Our results showed that CSBV was the most prevalent as it was detected in (90%), of the samples, DWV was detected in (81.25%), and IAPV was detected in (26.25%). In contrast, insignificant prevalence results were obtained from all apiaries for BQCV, CBPV, APBV, and KBV, which were not detected in any sample. Here, we are providing the first report on the molecular detection of honeybee viruses, especially the prevalence of IAPV, from different regions in the Fujian Province of China with a high prevalence of bee viruses, on A. c. cerana, and there is great concern for the presence of honeybee viruses in the population of the native honeybee (A. c. cerana) in China.

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“…In Korea, six AmSBVs (Table 1) [24,36]. In 2017, a comparative genomic analysis among nine SBVs of A. cerana and A. mellifera was performed in Vietnam [30]. These reports identified different genomic features and revealed the genetic diversity among these SBVs, suggesting that viral cross-infections might occur between AcSBV and AmSBV.…”

Section: Introductionmentioning

confidence: 99%

Genomic Sequencing and Comparison of Sacbrood Viruses from Apis cerana and Apis mellifera in Taiwan

Chang

et al. 2020

Pathogens

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Sacbrood virus (SBV) was the first identified bee virus and shown to cause serious epizootic infections in the population of Apis cerana in Taiwan in 2015. Herein, the whole genome sequences of SBVs in A. cerana and A. mellifera were decoded and designated AcSBV-TW and AmSBV-TW, respectively. The whole genomes of AcSBV-TW and AmSBV-TW were 8776 and 8885 bp, respectively, and shared 90% identity. Each viral genome encoded a polyprotein, which consisted of 2841 aa in AcSBV-TW and 2859 aa in AmSBV-TW, and these sequences shared 95% identity. Compared to 54 other SBVs, the structural protein and protease regions showed high variation, while the helicase was the most highly conserved region among SBVs. Moreover, a 17-amino-acid deletion was found in viral protein 1 (VP1) region of AcSBV-TW compared to AmSBV-TW. The phylogenetic analysis based on the polyprotein sequences and partial VP1 region indicated that AcSBV-TW was grouped into the SBV clade with the AC-genotype (17-aa deletion) and was closely related to AmSBV-SDLY and CSBV-FZ, while AmSBV-TW was grouped into the AM-genotype clade but branched independently from other AmSBVs, indicating that the divergent genomic characteristics of AmSBV-TW might be a consequence of geographic distance driving evolution, and AcSBV-TW was closely related to CSBV-FZ, which originated from China. This 17-amino-acid deletion could be found in either AcSBV or AmSBV in Taiwan, indicating cross-infection between the two viruses. Our data revealed geographic and host specificities between SBVs. The amino acid difference in the VP1 region might serve as a molecular marker for describing SBV cross-infection.

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Comparative Genomic Analysis for Genetic Variation in Sacbrood Virus of Apis cerana and Apis mellifera Honeybees From Different Regions of Vietnam

Cited by 10 publications

References 35 publications

Genetic and phylogenetic analysis of Chinese sacbrood virus isolates fromApis mellifera

Genetic and phylogenetic analysis of Chinese sacbrood virus isolates fromApis mellifera

Prevalence of bee viruses in Apis cerana cerana populations from different locations in the Fujian Province of China

Genomic Sequencing and Comparison of Sacbrood Viruses from Apis cerana and Apis mellifera in Taiwan

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