Comparative genomic analysis of bacteriocin-producing Weissella cibaria 110

Abstract

Weissella cibaria 110 was isolated from plaa-som, a Thai fermented fish product, and known to produce the weissellicin 110 bacteriocin. We carried out comprehensive comparative genomic analysis of W. cibaria 110 with four other non-bacteriocin-producing W. cibaria strains and identified potential antibiotic-resistant genes. We further identified a type III restriction-modification system, a TA system, and a bacteriocin gene cluster that are unique in W. cibaria 110. Genes related to bacteriocin biosynthesis are organized in clusters and are encoded with minimum genetic machinery consisting of structural cognate immunity genes, including ABC transporter and immunity protein. Finally, we predicted W. cibaria 110 to produce a class IId bacteriocin, weissellicin 110, which is 31 amino acids in length and contains a 21-amino-acid N-terminal leader peptide. This is the first bacteriocin-producing sequencing genome in W. cibaria, and we describe the difference between the bacteriocin-producing and non bacteriocin-producing strains from genome point of view.

Characterization of Weissella koreensis SK Isolated from Kimchi Fermented at Low Temperature (around 0 °C) Based on Complete Genome Sequence and Corresponding Phenotype

“…RAST analysis identified a gene encoding a hemolysin Ⅲ in W. koreensis SK. There are multiple reports that most Weissella species are vancomycin-resistant, as they harbor a D-alanine-D-alanine ligase gene [ 9 ]. The D-alanine-D-alanine ligase gene contributing to vancomycin resistance was also identified in W. koreensis SK.…”

“…Members of the Weissella genus have been reported as one of the major lactic acid bacteria (LAB) responsible for kimchi fermentation [ 8 ]. Among Weissella species, Weissella confusa and Weissella cibaria species are being extensively studied for their use as probiotics based on functional and genomic characteristics [ 2 , 9 ], whereas only few studies have been conducted on the functional, genomic, and metabolic features of W. koreensis [ 4 , 10 ].…”

Genome Sequence of Weissella cibaria M2, a Potential Probiotic Strain Isolated from the Feces of a Giant Panda

“…Furthermore, several strains of W. cibaria have been widely researched for their probiotic potential. Besides their general probiotic properties (adhesion and bile salt resistance) ( 2 ), these microorganisms can also confer protection against bacterial and fungal infection through bacteriocinogeny, inhibition of colonization, and prevention of inflammation ( 3 – 6 ). Additionally, they are known to be effective in maintaining host health by their antitoxicity, antitumor activity, and immunomodulatory properties ( 7 – 11 ).…”

The Genome Sequence of Weissella cibaria DmW_103, Isolated from Wild Drosophila

“…For example, W. cibaria produces bacteriocins that have been studied for potential use as meat and dairy preservatives (1). Also, W. cibaria isolates have been screened for both general probiotic properties (adhesion and bile salt resistance) (2) and for conferring protection against a specific skin disease, atopic dermatitis (3).…”