Comparative genomic analysis of Cohnella hashimotonis sp. nov. isolated from the International Space Station

Simpson, Anna; Eedara, V. V. Ramprasad; Singh, Nitin Kumar; Damle, Namita; Parker, Ceth W.; Karouia, Fathi; Mason, Christopher E.; Venkateswaran, Kasthuri

doi:10.3389/fmicb.2023.1166013

Cited by 4 publications

(1 citation statement)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The genome size of strain Cohnella sp. 56 VKM B-36720 (7.16 Mbp) is in the same range as strain NL03-T5, but has an unusually high GC content of 60.21% compared to other strains, although some Cohnella strains such as C. ginsengisoli DSM 18997 T (59.06% GC) and C. rhizosphaerae DSM 28161 T (59.48% GC) have similarly high GC contents ( Simpson et al, 2023 ). 5.24% of the 5,758 genes of strain Cohnella sp.…”

Section: Discussionmentioning

confidence: 99%

Identification of a novel xanthan-binding module of a multi-modular Cohnella sp. xanthanase

Han,

Baudrexl,

Ludwig

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

A new strain of xanthan-degrading bacteria identified as Cohnella sp. has been isolated from a xanthan thickener for food production. The strain was able to utilize xanthan as the only carbon source and to reduce the viscosity of xanthan-containing medium during cultivation. Comparative analysis of the secretomes of Cohnella sp. after growth on different media led to the identification of a xanthanase designated as CspXan9, which was isolated after recombinant production in Escherichia coli. CspXan9 could efficiently degrade the β-1,4-glucan backbone of xanthan after previous removal of pyruvylated mannose residues from the ends of the native xanthan side chains by xanthan lyase treatment (XLT-xanthan). Compared with xanthanase from Paenibacillus nanensis, xanthanase CspXan9 had a different module composition at the N- and C-terminal ends. The main putative oligosaccharides released from XLT-xanthan by CspXan9 cleavage were tetrasaccharides and octasaccharides. To explore the functions of the N- and C-terminal regions of the enzyme, truncated variants lacking some of the non-catalytic modules (CspXan9-C, CspXan9-N, CspXan9-C-N) were produced. Enzyme assays with the purified deletion derivatives, which all contained the catalytic glycoside hydrolase family 9 (GH9) module, demonstrated substantially reduced specific activity on XLT-xanthan of CspXan9-C-N compared with full-length CspXan9. The C-terminal module of CspXan9 was found to represent a novel carbohydrate-binding module of family CBM66 with binding affinity for XLT-xanthan, as was shown by native affinity polyacrylamide gel electrophoresis in the presence of various polysaccharides. The only previously known binding function of a CBM66 member is exo-type binding to the non-reducing fructose ends of the β-fructan polysaccharides inulin and levan.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of a novel xanthan-binding module of a multi-modular Cohnella sp. xanthanase

Han,

Baudrexl,

Ludwig

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

Validation List no. 214. Valid publication of new names and new combinations effectively published outside the IJSEM

Oren,

Göker

2023

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

Genomic, Functional, and Metabolic Enhancements in Multidrug-ResistantEnterobacter bugandensisFacilitating its Persistence and Succession in the International Space Station

Sengupta,

Sivabalan,

Singh

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Background: The International Space Station (ISS) stands as a testament to human achievement in space exploration. Within its highly controlled environment, characterized by microgravity, increased CO2 levels, and elevated solar radiation, microorganisms occupy a unique niche. These microbial inhabitants play a significant role in influencing the health and well-being of astronauts on board. One microorganism of particular interest in our study is Enterobacter bugandensis, primarily found in clinical specimens including human gastrointestinal tract, and also reported to exhibit pathogenic traits, leading to a plethora of infections. Results: Distinct from their Earth counterparts, ISS E. bugandensis strains have exhibited resistance mechanisms that categorize them within the ESKAPE pathogen group, a collection of pathogens recognized for their formidable resistance to antimicrobial treatments. During the two-year Microbial Tracking 1 mission, 12 strains of multidrug-resistant E. bugandensis were isolated from various locations within the ISS. We have carried out a comprehensive study to understand the genomic intricacies of ISS-derived E. bugandensis in comparison to terrestrial strains, with a keen focus on those associated with clinical infections. We aimed to unravel the evolutionary trajectories of pivotal genes, especially those contributing to functional adaptations and potential antimicrobial resistance. A hypothesis central to our study was that the singular stresses of the space environment, distinct from any on Earth, could be driving these genomic adaptations. In extending our investigation, we meticulously mapped the prevalence and distribution of E. bugandensis across the ISS over time. This temporal analysis provided insights into the persistence, succession, and potential patterns of colonization of E. bugandensis in space. Furthermore, by leveraging advanced analytical techniques, including metabolic modeling, we delved into the coexisting microbial communities alongside E. bugandensis in the ISS across multiple missions and spatial locations. This exploration revealed intricate microbial interactions, offering a window into the microbial ecosystem dynamics within the ISS. Conclusions: Our comprehensive analysis illuminated not only the ways these interactions sculpt microbial diversity but also the factors that might contribute to the potential dominance and succession of E. bugandensis within the ISS environment. The implications of these findings are twofold. Firstly, they shed light on microbial behavior, adaptation, and evolution in extreme, isolated environments. Secondly, they underscore the need for robust preventive measures, ensuring the health and safety of astronauts by mitigating risks associated with potential pathogenic threats.

show abstract

Comparative genomic analysis of Cohnella hashimotonis sp. nov. isolated from the International Space Station

Cited by 4 publications

References 85 publications

Identification of a novel xanthan-binding module of a multi-modular Cohnella sp. xanthanase

Identification of a novel xanthan-binding module of a multi-modular Cohnella sp. xanthanase

Validation List no. 214. Valid publication of new names and new combinations effectively published outside the IJSEM

Genomic, Functional, and Metabolic Enhancements in Multidrug-ResistantEnterobacter bugandensisFacilitating its Persistence and Succession in the International Space Station

Contact Info

Product

Resources

About