Comparative Genomic Analysis of Cold-Water Coral-Derived Sulfitobacter faviae: Insights into Their Habitat Adaptation and Metabolism

Lin, Shituan; Guo, Yunxue; Huang, Zheng‐Ming; Tang, Kaihao; Wang, Pengxia

doi:10.3390/md21050309

Cited by 4 publications

(1 citation statement)

References 106 publications

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“…Sulfitobacter bacteria were first discovered in 1995 from the H 2 S-O 2 interface of the Black Sea and were described as Gram-negative, strictly aerobic, heterotrophic bacteria that were capable of sulfite oxidation [1]. Subsequently, Sulfitobacter bacteria were isolated from various habitats, including marine sediments, tidal flats, the Arctic, Antarctic Ekho Lake, the Mediterranean Sea, deep seawater, marine phycosphere, coral, and sea grass [2][3][4][5][6]. This suggests that the bacteria of this genus can adapt to a very broad range of marine environments.…”

Section: Introductionmentioning

confidence: 99%

Genomic Analysis of Novel Sulfitobacter Bacterial Strains Isolated from Marine Biofilms

Cui,

Fan,

Ding

et al. 2024

Marine Drugs

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Bacteria from the genus Sulfitobacter are distributed across various marine habitats and play a significant role in sulfur cycling. However, the metabolic features of Sulfitobacter inhabiting marine biofilms are still not well understood. Here, complete genomes and paired metatranscriptomes of eight Sulfitobacter strains, isolated from biofilms on subtidal stones, have been analyzed to explore their central energy metabolism and potential of secondary metabolite biosynthesis. Based on average nucleotide identity and phylogenetic analysis, the eight strains were classified into six novel species and two novel strains. The reconstruction of the metabolic pathways indicated that all strains had a complete Entner–Doudoroff pathway, pentose phosphate pathway, and diverse pathways for amino acid metabolism, suggesting the presence of an optimized central carbon metabolism. Pangenome analysis further revealed the differences between the gene cluster distribution patterns among the eight strains, suggesting significant functional variation. Moreover, a total of 47 biosynthetic gene clusters were discovered, which were further classified into 37 gene cluster families that showed low similarity with previously documented clusters. Furthermore, metatranscriptomic analysis revealed the expressions of key functional genes involved in the biosynthesis of ribosomal peptides in in situ marine biofilms. Overall, this study sheds new light on the metabolic features, adaptive strategies, and value of genome mining in this group of biofilm-associated Sulfitobacter bacteria.

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Section: Introductionmentioning

confidence: 99%

Genomic Analysis of Novel Sulfitobacter Bacterial Strains Isolated from Marine Biofilms

Cui,

Fan,

Ding

et al. 2024

Marine Drugs

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Complete genome analysis of deep-sea hydrothermal sulfur-oxidizing bacterium Sulfitobacter sp. TCYB15 associated with mussel Bathymodiolus marisindicus and insights into its habitat adaptation

Deng,

Zhu,

et al. 2024

Marine Genomics

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Stronger together: harnessing natural algal communities as potential probiotics for inhibition of aquaculture pathogens

Smahajcsik,

Roager,

Strube

et al. 2024

Preprint

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Intensive fish rearing in aquaculture is challenged by infectious diseases, and although vaccines have been successfully developed for mature fish, alternative disease control measures are needed for fish larvae and juveniles. Probiotics offer a promising alternative to antibiotics, with the potential to reduce the risk of antibiotic resistance. Probiotics are typically isolated and used as pure cultures, however, in natural environments it is the concerted effort of the complex microbiome that keeps pathogens at bay. Here, we developed an in vitro assay to evaluate the anti-pathogen efficacy of mixed algal microbiomes from the live-feed microalgae Tetraselmis suecica and Isochrysis galbana. The inhibition of a GFP-tagged Vibrio anguillarum, a key fish pathogen, by microbial communities, was measured and quantified as reduction in fluorescence. The Isochrysis galbana microbiome was more inhibitory to V. anguillarum than the Tetraselmis suecica microbiome. During co-culture with the pathogen, the bacterial density of the Isochrysis microbiomes increased whilst the diversity was reduced as determined by metataxonomic analyses. Bacteria isolated from the fully inhibitory microbiomes were members of Alteromonadaceae, Halomonadaceae, Rhodobacteraceae, Vibrionaceae, Flavobacteriaceae, and Erythrobacteraceae. Although some strains individually inhibited the pathogen, these were not the key members of the microbiome and enhanced inhibition was observed when Sulfitobacter pontiacus D3 and Halomonas campaniensis D2 were co-cultured, even though neither were inhibitory as monocultures. Thus, this study demonstrates that microbial communities derived from natural algal microbiomes can have anti-pathogen effects, and that bacterial co-cultures may offer synergistic advantages over monocultures as probiotics, highlighting their promise for aquaculture health strategies.

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Comparative Genomic Analysis of Cold-Water Coral-Derived Sulfitobacter faviae: Insights into Their Habitat Adaptation and Metabolism

Cited by 4 publications

References 106 publications

Genomic Analysis of Novel Sulfitobacter Bacterial Strains Isolated from Marine Biofilms

Genomic Analysis of Novel Sulfitobacter Bacterial Strains Isolated from Marine Biofilms

Complete genome analysis of deep-sea hydrothermal sulfur-oxidizing bacterium Sulfitobacter sp. TCYB15 associated with mussel Bathymodiolus marisindicus and insights into its habitat adaptation

Stronger together: harnessing natural algal communities as potential probiotics for inhibition of aquaculture pathogens

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