Comparative genomic analysis of diverse rhizobia and effective nitrogen-fixing clover-nodulating Rhizobium strains adapted to Egyptian dry ecosystems

Shamseldin, Abdelaal; Epstein, Brendan; Sadowsky, Michael J.; Zhang, Qian

doi:10.1007/s13199-021-00764-6

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…Furthermore, Castaingts et al [40] identified Pvu-miR5942 as a newly discovered miRNA that plays a role in selecting Rhizobium etli strains for nodule colonization. Furthermore, Rhizobium etli CFN42 has the capacity to form a symbiotic association with the roots of Phaseolus vulgaris plants, resulting in the development of nitrogen-fixing nodules [41][42][43], in clover [44,45], and in Faba bean (Vicia faba L.), T. semipilosum, and white clover (T. repens L.). It seems that R. etli has high compatibility to nodulate various species of clover.…”

Section: Discussionmentioning

confidence: 99%

Insights into Genetic and Physiological Characteristics of Clover Rhizobia in Afghanistan Soils

Habibi,

Yasuda,

Aryan

et al. 2024

Applied Microbiology

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Livestock production in Afghanistan highly relies on grazing and clover feed, which is a key component of pastures and forage crops. This study elucidated the genetic diversity of clover-nodulating rhizobia in different ecological regions and their effects on clover growth. A total of 57 rhizobia were isolated and their genetic diversities were studied through 16S rRNA and nifD genes. The isolates were inoculated to clover (Afghan local variety), to investigate the potential of nitrogen fixation and influences of clover growth. The 16S rRNA gene analysis showed two distinct groups of Rhizobium (94.7%) and Ensifer (5.3%) species. The nifD phylogenetic relationship revealed a high similarity to Rhizobium and a novel lineage group close to Rhizobium leguminosarum species. In the plant test, different genotypes significantly (p < 0.01) exhibited an increase in plant biomass production, compared to the un-inoculated plants. Among genotypes, the highest plant biomass was recorded in PC8 (1769.0 mg/plant) and PC9 (1409.2 mg/plant) isolates as compared to un-inoculated plants (144.0 mg/plant). Moreover, these isolates showed maximum nitrogen fixation rates of 8.2 and 6.5 µM/plant, respectively. These isolates were identified as the most promising rhizobial strains for developing biofertilizers in the context of Afghanistan.

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Section: Discussionmentioning

confidence: 99%

Insights into Genetic and Physiological Characteristics of Clover Rhizobia in Afghanistan Soils

Habibi,

Yasuda,

Aryan

et al. 2024

Applied Microbiology

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“…It is suitable for silaging, haying, and grazing for livestock due to its high quality (Acharya et al, 2011). Meanwhile, white clover can also maintain soil fertility by providing nitrogen from its symbiotic interactions with rhizobia (Shamseldin et al, 2021). Additionally, it has well-developed and numerous stolons and/or shoots that are beneficial for water and soil conservation (Ballhorn and Elias, 2014;Zhang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Newly Isolated Paenibacillus monticola sp. nov., a Novel Plant Growth-Promoting Rhizobacteria Strain From High-Altitude Spruce Forests in the Qilian Mountains, China

Gan

Yue

et al. 2022

Front. Microbiol.

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Species in the genus Paenibacillus from special habitats have attracted great attention due to their plant growth-promoting traits. A novel plant growth-promoting rhizobacteria (PGPR) species in the genus Paenibacillus was isolated from spruce forest at the height of 3,150 m in the Qilian Mountains, Gansu province, China. The phylogenetic analysis based on 16S rRNA, rpoB, and nifH gene sequences demonstrated that strain LC-T2T was affiliated in the genus Paenibacillus and exhibited the highest sequence similarity with Paenibacillus donghaensis KCTC 13049T (97.4%). Average nucleotide identity (ANIb and ANIm) and digital DNA–DNA hybridization (dDDH) between strain LC-T2T and P. donghaensis KCTC 13049T were 72.6, 83.3, and 21.2%, respectively, indicating their genetic differences at the species level. These differences were further verified by polar lipids profiles, major fatty acid contents, and several distinct physiological characteristics. Meanwhile, the draft genome analysis provided insight into the genetic features to support its plant-associated lifestyle and habitat adaptation. Subsequently, the effects of volatile organic compound (VOC) emitted from strain LC-T2T on the growth of Arabidopsis were evaluated. Application of strain LC-T2T significantly improved root surface area, root projection area, and root fork numbers by 158.3, 158.3, and 241.2%, respectively, compared to control. Also, the effects of LC-T2T on the growth of white clover (Trifolium repens L.) were further assessed by pot experiment. Application of LC-T2T also significantly improved the growth of white clover with root fresh weight increased over three-folds compared to control. Furthermore, the viable bacterial genera of rhizosphere soil were detected in each treatment. The number of genera from LC-T2T-inoculated rhizosphere soil was 1.7-fold higher than that of control, and some isolates were similar to strain LC-T2T, indicating that LC-T2T inoculation was effective in the rhizosphere soil of white clover. Overall, strain LC-T2T should be attributed to a novel PGPR species within the genus Paenibacillus based on phylogenetic relatedness, genotypic features, and phenotypic and inoculation experiment, for which the name Paenibacillus monticola sp. nov. is proposed.

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How to manage a scientific project in biological nitrogen fixation (BNF) and the topics that needed to be searched?

Shamseldin

2023

Bull Natl Res Cent

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Planning to have a good scientific project generally or specifically in BNF is a main target for young scientists and PhD students worldwide. Therefore, it is hardly urgent to well manage the project, which defined the use of knowledge, skills and methods to achieve objectives of the project in the determined time without delay. Consequently, in this letter I will explain the main points that needed to be covered to manage a research project in BNF and which subjects of research are requested to be covered in the future.

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Comparative genomic analysis of diverse rhizobia and effective nitrogen-fixing clover-nodulating Rhizobium strains adapted to Egyptian dry ecosystems

Cited by 3 publications

References 18 publications

Insights into Genetic and Physiological Characteristics of Clover Rhizobia in Afghanistan Soils

Insights into Genetic and Physiological Characteristics of Clover Rhizobia in Afghanistan Soils

Newly Isolated Paenibacillus monticola sp. nov., a Novel Plant Growth-Promoting Rhizobacteria Strain From High-Altitude Spruce Forests in the Qilian Mountains, China

How to manage a scientific project in biological nitrogen fixation (BNF) and the topics that needed to be searched?

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