Comparative genomic analysis of Lactobacillus plantarum ZJ316 reveals its genetic adaptation and potential probiotic profiles

Abstract

Abstract:Objective: In previous studies, Lactobacillus plantarum ZJ316 showed probiotic properties, such as antimicrobial activity against various pathogens and the capacity to significantly improve pig growth and pork quality. The purpose of this study was to reveal the genes potentially related to its genetic adaptation and probiotic profiles based on comparative genomic analysis. Methods: The genome sequence of L. plantarum ZJ316 was compared with those of eight L. plantarum strains deposited in GenBank. BLASTN, Mauve, and MUMmer programs were used for genome alignment and comparison. CRISPRFinder was applied for searching the clustered regularly interspaced short palindromic repeats (CRISPRs). Results: We identified genes that encode proteins related to genetic adaptation and probiotic profiles, including carbohydrate transport and metabolism, proteolytic enzyme systems and amino acid biosynthesis, CRISPR adaptive immunity, stress responses, bile salt resistance, ability to adhere to the host intestinal wall, exopolysaccharide (EPS) biosynthesis, and bacteriocin biosynthesis. Conclusions: Comparative characterization of the L. plantarum ZJ316 genome provided the genetic basis for further elucidating the functional mechanisms of its probiotic properties. ZJ316 could be considered a potential probiotic candidate.

Complete genome sequencing of exopolysaccharide-producing Lactobacillus plantarum K25 provides genetic evidence for the probiotic functionality and cold endurance capacity of the strain

“…Total 2713 CDSs are divided into 22 groups using COG class description ( Figure 2). Comparing with other strains of L. plantarum [11], strain K25 has larger numbers of genes in category V, J, D, T, N, O, H, F, I, W and X, and smaller numbers of genes in category E, P and L. L. plantarum K25 was weakly proteolytic in Cheddar cheese during ripening [20], probably due to its lack of genes for amino acid transport and metabolism, such as pepL (proline-specific peptidase) and pepQ (Xaa-Pro dipeptidase).…”

“…EPSs are extracellular biopolymers, which could act as cryoprotectant, host immune stimulating factor and antibacterial agent [11]. L. plantarum K25 produced 311.08 ± 30.5 mg/L of EPS in SDM medium at 37 °C for 24 h (unpublished data).…”

“…The cer7B belongs to class IIb bacteriocin lactobin A/cerein 7B family [44]. The conserved region contains the operons plnEFI and plnJL that encode plantaricins I and L, plnABCD that is responsible for the signal transduction, and pln GHSTUVW that is highly conserved in the pln gene locus, encoding ABC transporter and the secreted proteins [4,11]. There are four permeases (B5G53_0276, B5G53_2317, B5G53_2388 and B5G53_2421) of ABCtype antimicrobial peptide transport system in the chromosome.…”

“…The ftsH encoded a chaperone protease for stress resistance; the ctsR encoded a Class III stressor to control the ftsH expression; the hsp encoded a heat shock protein participating in membrane fluidity [10]. Bile hydrolases were considered to be important for bacterial survival in the intestinal tract [1, 11,12]. L. plantarum carried four bile salt hydrolase (bsh1, bsh2, bsh3, and bsh4) genes being responsible for bile salt hydrolyzing activity [13][14][15][16].…”

Antimicrobial Effects of Probiotics and Novel Probiotic-Based Approaches for Infectious Diseases

“…The ability of probiotics to establish in the gastrointestinal (GI) tract, maintain flora healthy, and reduce the growth of pathogens and colonization is enhanced by their ability to eliminate competitors. Probiotic strains release different antimicrobial molecules such as organic acids, hydrogen peroxide (Н 2 О 2 ), and antimicrobial peptide bacteriocins into the intestinal environment to limit the growth of bacterial and fungal pathogens [6,[39][40][41][42][43].…”