Comparative genomic analysis of the Dietzia genus: an insight into genomic diversity, and adaptation

Santos, Roselane Gonçalves dos; Castillo, Raquel Hurtado; Rodrigues, Diego Lucas Neres; Silva, Alessandra Lima da; Anjos, William Ferreira dos; Rifici, Claudia; Attili, Annarita; Tiwari, Sandeep; Jaiswal, Arun Kumar; Spier, Sharon J.; Mazzullo, G.; Morais-Rodrigues, Francielly; Gomide, Anne Cybelle Pinto; Jesus, Luís Cláudio Lima de; Aburjaile, Flávia Figueira; Brenig, Bertram; Cuteri, Vincenzo; Castro, Thiago Luiz de Paula; Seyffert, Núbia; Santos, Anderson; Goés-Neto, Aristóteles; Sousa, Thiago J.; Azevedo, Vasco

doi:10.1016/j.resmic.2022.103998

Cited by 4 publications

(1 citation statement)

References 71 publications

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“…This study showed that the 16S rRNA gene of the isolates exhibited the highest homology with that of B. porcorum but fairly low similarity to that of B. zoohelcum and B. porcorum. ANI and isDDH are the traditional "gold standards" for circumscribing a bacterial species, and pairwise comparisons of strains with ANI values ≥ 95% and isDDH values ≥ 70% are typically considered to indicate the same species [48]. In this study, lower ANI and isDDH values were detected between the isolated strain and B. zoohelcum, and between the isolated strain and B. cardium strains, which is consistent with the findings of the phylogenetic analyses and indicates that the isolated strain belongs to B. porcorum.…”

Section: Discussionmentioning

confidence: 99%

Characterization and the first complete genome sequence of a novel strain of Bergeyella porcorum isolated from pigs in China

Liu,

Chen,

Jiang

et al. 2024

BMC Microbiol

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Background Bergeyella porcorum is a newly identified bacterium that has an ambiguous relationship with pneumonia in pigs. However, few studies have adequately characterized this species. Results In this study, we analyzed the morphological, physiological, and genomic characteristics of the newly identified B. porcorum sp. nov. strain QD2021 isolated from pigs. The complete genome sequence of the B. porcorum QD2021 strain consists of a single circular chromosome (2,271,736 bp, 38.51% G + C content), which encodes 2,578 genes. One plasmid with a size of 70,040 bp was detected. A total of 121 scattered repeat sequences, 319 tandem repeat sequences, 4 genomic islands, 5 prophages, 3 CRISPR sequences, and 51 ncRNAs were predicted. The coding genes of the B. porcorum genome were successfully annotated across eight databases (NR, GO, KEGG, COG, TCDB, Pfam, Swiss-Prot and CAZy) and four pathogenicity-related databases (PHI, CARD, VFDB and ARDB). In addition, a comparative genome analysis was performed to explore the evolutionary relationships of B. porcorum QD2021. Conclusions To our knowledge, this is the first study to provide fundamental phenotypic and whole-genome sequences for B. porcorum. Our results extensively expand the current knowledge and could serve as a valuable genomic resource for future research on B. porcorum.

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Section: Discussionmentioning

confidence: 99%

Characterization and the first complete genome sequence of a novel strain of Bergeyella porcorum isolated from pigs in China

Liu,

Chen,

Jiang

et al. 2024

BMC Microbiol

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Investigating the genomic and metabolic abilities of PGPR Pseudomonas fluorescens in promoting plant growth and fire blight management

Mankoti,

Pandit,

Meena

et al. 2024

Mol Genet Genomics

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Screening and Identification of Protease-Producing Microorganisms in the Gut of Gryllotalpa orientalis (Orthoptera: Gryllotalpidae)

Zheng,

Zhao,

et al. 2024

Insects

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The insect gut harbors a diverse array of functional microorganisms that warrant further exploration and utilization. However, there is currently a paucity of research reports on the discovery of protease-producing microorganisms with industrial application value in the gut. Here, we employed microbial culturing to screen and identify the protease-producing microorganisms in the gut extract of Gryllotalpa orientalis. Based on morphological, physiological, and biochemical characterization, 16S rRNA sequencing, as well as ANI and dDDH values of whole genome, the protease-producing strains isolated from the insect gut were identified as Priestia aryahattai DBM-1 and DX-4, P. megaterium DX-3, and Serratia surfactantfaciens DBM-5. According to whole-genome analysis, strain DBM-5, which exhibited the highest enzyme activity, possesses abundant membrane transport genes and carbohydrate metabolism enzymes. In contrast, strains DX-3 and DX-4 not only have the ability to hydrolyze proteins but also demonstrate the capability to hydrolyze plant materials. Furthermore, strains that are closely related tend to have similar metabolic product gene clusters in their genomes. The screening and identification of protease resources are essential for the subsequent development and utilization of gut functional microorganisms and genetic resources in insects.

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Comparative genomic analysis of the Dietzia genus: an insight into genomic diversity, and adaptation

Cited by 4 publications

References 71 publications

Characterization and the first complete genome sequence of a novel strain of Bergeyella porcorum isolated from pigs in China

Characterization and the first complete genome sequence of a novel strain of Bergeyella porcorum isolated from pigs in China

Investigating the genomic and metabolic abilities of PGPR Pseudomonas fluorescens in promoting plant growth and fire blight management

Screening and Identification of Protease-Producing Microorganisms in the Gut of Gryllotalpa orientalis (Orthoptera: Gryllotalpidae)

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