Comparative Genomics of Acetic Acid Bacteria within the Genus Bombella in Light of Beehive Habitat Adaptation

Härer, Luca; Hilgarth, Maik; Ehrmann, Matthias A.

doi:10.3390/microorganisms10051058

Cited by 7 publications

(7 citation statements)

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“…The genomes of these two bacteria, along with Snodgrassella , Bartonella , Lactobacillus , and Bombilactobacillus , encode genes that catalyze the reactions of a wide variety of polysaccharides and monosaccharides, including pectin-degrading enzymes and glycoside hydrolases, and therefore have the potential to aid in the breakdown of pollen, the release of nutrient-rich components thereof, and the removal of toxic sugars [ 99 , 100 ]. In contrast, Apibacter [ 101 , 102 ] and Bombella [ 103 , 104 ] genomes mainly encode enzymes for the utilization of simple mono-saccharides or sucrose. Bee symbionts also have diverging capacities for the biosynthesis of amino acids and other vitamins.…”

Section: Resultsmentioning

confidence: 99%

A phylogenomic and comparative genomic analysis of Commensalibacter, a versatile insect symbiont

et al. 2023

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Background To understand mechanisms of adaptation and plasticity of pollinators and other insects a better understanding of diversity and function of their key symbionts is required. Commensalibacter is a genus of acetic acid bacterial symbionts in the gut of honey bees and other insect species, yet little information is available on the diversity and function of Commensalibacter bacteria. In the present study, whole-genome sequences of 12 Commensalibacter isolates from bumble bees, butterflies, Asian hornets and rowan berries were determined, and publicly available genome assemblies of 14 Commensalibacter strains were used in a phylogenomic and comparative genomic analysis. Results The phylogenomic analysis revealed that the 26 Commensalibacter isolates represented four species, i.e. Commensalibacter intestini and three novel species for which we propose the names Commensalibacter melissae sp. nov., Commensalibacter communis sp. nov. and Commensalibacter papalotli sp. nov. Comparative genomic analysis revealed that the four Commensalibacter species had similar genetic pathways for central metabolism characterized by a complete tricarboxylic acid cycle and pentose phosphate pathway, but their genomes differed in size, G + C content, amino acid metabolism and carbohydrate-utilizing enzymes. The reduced genome size, the large number of species-specific gene clusters, and the small number of gene clusters shared between C. melissae and other Commensalibacter species suggested a unique evolutionary process in C. melissae, the Western honey bee symbiont. Conclusion The genus Commensalibacter is a widely distributed insect symbiont that consists of multiple species, each contributing in a species specific manner to the physiology of the holobiont host.

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Section: Resultsmentioning

confidence: 99%

A phylogenomic and comparative genomic analysis of Commensalibacter, a versatile insect symbiont

et al. 2023

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“…Honeybee endogenous Fructobacilli regulate larval and pupal storage proteins (hexamerin 70b), which contribute to developmental stages [ 50 ]. Acetic acid bacteria of the genus Bombella colonized various niches in beehives and are associated with larval protection against microbial pathogens [ 51 ]. It is worth noting that developing larvae have a discontinuous gut (the foregut is not connected to the hindgut) before pupation [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Gut Microbiota of Apis mellifera at Selected Ontogenetic Stages and Their Immunogenic Potential during Summer

Maigoro,

Lee,

Kim

et al. 2024

Pathogens

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Honeybees (Apis mellifera) are pollinating agents of economic importance. The role of the gut microbiome in honeybee health has become increasingly evident due to its relationship with immune function, growth, and development. Although their dynamics at various developmental stages have been documented, their dynamics during the era of colony collapse disorder and immunogenic potential, which are connected to the antagonistic immune response against pathogens, need to be elucidated. Using 16S rRNA gene Illumina sequencing, the results indicated changes in the gut microbiota with the developmental stage. The bacterial diversity of fifth stage larva was significantly different among the other age groups, in which Fructobacillus, Escherichia-Shigella, Bombella, and Tyzzerella were unique bacteria. In addition, the diversity of the worker bee microbiome was distinct from that of the younger microbiome. Lactobacillus and Gilliamella remained conserved throughout the developmental stages, while Bifidobacterium colonized only worker bees. Using an in silico approach, the production potential of lipopolysaccharide-endotoxin was predicted. Forager bees tend to have a higher abundance rate of Gram-negative bacteria. Our results revealed the evolutionary importance of some microbiome from the larval stage to the adult stage, providing insight into the potential dynamics of disease response and susceptibility. This finding provides a theoretical foundation for furthering the understanding of the function of the gut microbiota at various developmental stages related to probiotic development and immunogenic potential.

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“…The genus Bombella is part of the family of Acetobacteraceae ( Alphaproteobacteria ), more precisely of the clade of acetic acid bacteria (AAB) [1]. Species of the genus Bombella are described as symbionts of honey bees and highly adapted to high-sugar environments [2]. The host profits from the symbiosis in nutritional aspects and pathogen protection [3, 4].…”

Section: Full-textmentioning

confidence: 99%

“…Strains were grown on LMG 404 agar plates at temperatures between 10 °C and 45 °C at 5 °C intervals to determine temperature tolerance and optimum. Growth was observed after 24, 48 and 72 h. Growth at different pH values and glucose concentrations was tested in 96-well plates over 72 h in a plate photometer (adapted from [2], SPECTROstar nano , BMG Labtech). The pH range tested was between 3.5 and 10 at 1 pH unit intervals in buffered LMG404 media.…”

Section: Physiologymentioning

confidence: 99%

“…All strains of the not validly published species Parasaccharibacter apium have been reclassified as Bombella apis [7]. It has also been reported that the isolates ESL0385, ESL0378 and AS1 represent two as yet undescribed species of the genus [2,[6][7][8]. Several hundred bacterial isolates from the honey bee environment were analysed to investigate the diversity of members of the genus Bombella in the environment of the western honey bee Apis mellifera.…”

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Bombella pluederhausensis sp. nov., Bombella pollinis sp. nov., Bombella saccharophila sp. nov. and Bombella dulcis sp. nov., four Bombella species isolated from the environment of the western honey bee Apis mellifera

Härer,

Stýblová,

Ehrmann

2023

International Journal of Systematic and Evolutionary Microbiology

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Four strains of members of the genus Bombella were isolated from samples associated with the western honey bee Apis mellifera, which could not be assigned to a species with a validly published name. Strains TMW 2.2543T, TMW 2.2556T, TMW 2.2558T and TMW 2.2559T exhibit in silico DNA–DNA hybridisation (isDDH) and orthologous average nucleotide identity (orthoANI) values below species delineation thresholds compared with all described species of the genus Bombella and with each other. TMW 2.2556T and TMW 2.2558T form their own clade within the genus. The major respiratory quinone of all strains was Q-10. The composition of cellular fatty acids was diverse between strains. All strains stained Gram-negative, were rod-shaped, strictly aerobic, pellicle-forming, catalase-positive, oxidase-negative, mesophilic and grew over a wide pH range; they were halosensitive but glucose-tolerant. Unlike the other studied strains, TMW 2.2558T was non-motile. Phylogenetic, chemotaxonomic and physiological analyses revealed a clear distinction between all the strains and species with validly published names. All the data support the proposition of four novel species within the genus Bombella , namely Bombella pluederhausensis sp. nov., Bombella pollinis sp. nov., Bombella saccharophila sp. nov. and Bombella dulcis sp. nov., with the respective type strains Bombella pluederhausensis sp. nov. TMW 2.2543T (= DSM 114872T, = LMG 32791T), Bombella pollinis sp. nov. TMW 2.2556T (= DSM 114874T, = LMG 32792T), Bombella saccharophila sp. nov. TMW 2.2558T (= DSM 114875T, = LMG 32793T) and Bombella dulcis sp. nov. TMW 2.2559T (= DSM 114877T, = LMG 32794T). Moreover, three genomes available in the NCBI database that have not yet been described as species with validly published names could be assigned to the proposed species. Bombella sp. ESL0378 and Bombella sp. ESL0385 to Bombella pollinis sp. nov. and Bombella sp. AS1 to Bombella saccharophila sp. nov.

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Comparative Genomics of Acetic Acid Bacteria within the Genus Bombella in Light of Beehive Habitat Adaptation

Cited by 7 publications

References 63 publications

A phylogenomic and comparative genomic analysis of Commensalibacter, a versatile insect symbiont

A phylogenomic and comparative genomic analysis of Commensalibacter, a versatile insect symbiont

Gut Microbiota of Apis mellifera at Selected Ontogenetic Stages and Their Immunogenic Potential during Summer

Bombella pluederhausensis sp. nov., Bombella pollinis sp. nov., Bombella saccharophila sp. nov. and Bombella dulcis sp. nov., four Bombella species isolated from the environment of the western honey bee Apis mellifera

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