Comparative Genomics of Canadian Epidemic Lineages of Methicillin-Resistant
            <i>Staphylococcus aureus</i>

Christianson, Sara; Golding, George R.; Campbell, Jennifer; Mulvey, Michael R.

doi:10.1128/jcm.02500-06

Cited by 82 publications

(88 citation statements)

References 22 publications

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“…When bsa genes were first identified, it was suggested that possession of these genes might represent an alternative distinctive feature of CA-MRSA isolates. However, as is evident from analyses of genome-sequenced strains (as described above) and comparative genomics studies, an exact correlation does not exist (8). Nonetheless, the production of the Bsa lantibiotic by CA-MRSA strains may be significant given the virulent nature of many of these isolates, in particular the PVL-positive (PVL ϩ ) forms due to their need to be more competitive than their HA-MRSA counterparts with respect to flourishing on the skin surface.…”

Section: Vol 192 2010mentioning

confidence: 99%

Production of the Bsa Lantibiotic by Community-Acquired Staphylococcus aureus Strains

Daly¹,

Upton

Sandiford

et al. 2010

J Bacteriol

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Lantibiotics are antimicrobial peptides that have been the focus of much attention in recent years with a view to clinical, veterinary, and food applications. Although many lantibiotics are produced by food-grade bacteria or bacteria generally regarded as safe, some lantibiotics are produced by pathogens and, rather than contributing to food safety and/or health, add to the virulence potential of the producing strains. Indeed, genome sequencing has revealed the presence of genes apparently encoding a lantibiotic, designated Bsa (bacteriocin of Staphylococcus aureus), among clinical isolates of S. aureus and those associated with community-acquired methicillin-resistant S. aureus (MRSA) infections in particular. Here, we establish for the first time, through a combination of reverse genetics, mass spectrometry, and mutagenesis, that these genes encode a functional lantibiotic. We also reveal that Bsa is identical to the previously identified bacteriocin staphylococcin Au-26, produced by an S. aureus strain of vaginal origin. Our examination of MRSA isolates that produce the Panton-Valentine leukocidin demonstrates that many community-acquired S. aureus strains, and representatives of ST8 and ST80 in particular, are producers of Bsa. While possession of Bsa immunity genes does not significantly enhance resistance to the related lantibiotic gallidermin, the broad antimicrobial spectrum of Bsa strongly indicates that production of this bacteriocin confers a competitive ecological advantage on communityacquired S. aureus.

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Section: Vol 192 2010mentioning

confidence: 99%

Production of the Bsa Lantibiotic by Community-Acquired Staphylococcus aureus Strains

Daly¹,

Upton

Sandiford

et al. 2010

J Bacteriol

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“…The mapping of bacterial clones confirmed the hypothesis of occupational exposure in horse medicine and food inspection [16,17] while nothing similar has been done in veterinarians working in pig farming.…”

Section: Introductionmentioning

confidence: 55%

Interspecific Epidemiology of MRSA in Pig Farming

2014

J Infect Dis Ther

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The populations of MRSA are classified in relation to their origin, distinguishing S. aureus Healthcare-Acquired aureus (HA), Community-Acquired (CA) and Livestock-Acquired (LA). For LA-MRSA livestock animals have an important epidemiological role. This has raised the suspicion that the intensive husbandry may be, for the frequency and intensity of use of antibacterial treatments, an elective field of clonal selection of antibiotic resistance. The presence of LA-MRSA in pig production is sure in many countries and the pig is considered a reservoir for transmission to humans and other animals. In fact, farmers and production workers have a higher rate of colonization than the rest of the population. This work reports the presence of MRSA in slaughter pigs (2.3% positivity of the tonsils), sows (10% positivity of nasal swabs) and veterinarians employed in pig production (25% of nasal carriers). Many of the isolates from pigs and man belong to the same genomic patterns.

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“…By comparison, CA-MRSA carried only SCCmec type IV, were predominantly PVL-positive and belonged to either the CMRSA7 (USA400) or CMRSA10 (USA300) genotypes. CMRSA7 (USA400) and CMRSA10 (USA300) are the predominant CA-MRSA strains circulating in North America and, although not as prevalent as they are in many centres in the United States, have been increasingly isolated in Canada since 2004 (8,18,19). Presence of the PVL toxin has frequently been linked to CA-MRSA infections and is hypothesized to play a significant role in increased disease severity (2,20).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Community‐Associated and Health Care‐Associated Methicillin‐Resistant Staphylococcus aureus in Canada: Results from CANWARD 2007

Nichol

McCracken

DeCorby

et al. 2009

Canadian Journal of Infectious Diseases and Medical Microbiolog

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BACKGROUND: Community-associated methicillin-resistantStaphylococcus aureus(CA-MRSA) differ from health care-associated MRSA (HA-MRSA) in their genotypic and phenotypic characteristics. The purpose of the present study was to compare the demographics, antimicrobial susceptibilities and molecular epidemiology of CA-MRSA and HA-MRSA in Canada. METHODS: In 2007, 385 MRSA isolates were collected from Canadian patients attending hospital clinics, emergency rooms, medical/ surgical wards and intensive care units. Susceptibilities to betalactams, clarithromycin, clindamycin, daptomycin, levofloxacin, linezolid, moxifloxacin, tigecycline, trimethoprim-sulfamethoxazole and vancomycin were determined by Clinical and Laboratory Standards Institute broth microdilution. Strain typing was performed by pulsed-field gel electrophoresis (PFGE) and themecA,nucandpvlgenes were detected by polymerase chain reaction. RESULTS: Of the 385 MRSA, 19.5% were CA-MRSA and 79.2% were HA-MRSA as determined by PFGE. CA-MRSA belonged to PFGE types CMRSA10/USA300 (66.7%) and CMRSA7/USA400 (33.3%); PFGE types identified among HA-MRSA included CMRSA2/USA100/800 (81.6%), CMRSA6 (13.1%), CMRSA1/ USA600 (3.3%), CMRSA5/USA500 (1.3%), CMRSA3 (0.3%) and CMRSA9 (0.3%). Panton-Valentine leukocidin (PVL) was detected in 94.7% of CA-MRSA and 0.7% of HA-MRSA. Resistance rates (CA-MRSA versus HA-MRSA) were 61.3% versus 97.7% to levofloxacin, 73.3% versus 96.7% to clarithromycin, 12.0% versus 74.8% to clindamycin and 0.0% versus 15.4% to trimethoprim-sulfamethoxazole. No MRSA were resistant to vancomycin, linezolid, tigecycline or daptomycin. CONCLUSIONS: CA-MRSA represented 19.5% of all MRSA. CA-MRSA was significantly more susceptible to levofloxacin, clarithromycin, clindamycin and trimethoprim-sulfamethoxazole than HA-MRSA. Of CA-MRSA, 94.7% were PVL-positive while 99.3% of HA-MRSA were PVL-negative. CA-MRSA is an emerging pathogen in Canadian hospitals.

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Comparative Genomics of Canadian Epidemic Lineages of Methicillin-Resistant Staphylococcus aureus

Cited by 82 publications

References 22 publications

Production of the Bsa Lantibiotic by Community-Acquired Staphylococcus aureus Strains

Production of the Bsa Lantibiotic by Community-Acquired Staphylococcus aureus Strains

Interspecific Epidemiology of MRSA in Pig Farming

Comparison of Community‐Associated and Health Care‐Associated Methicillin‐Resistant Staphylococcus aureus in Canada: Results from CANWARD 2007

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