Comparative genomics of MRSA strains from human and canine origins reveals similar virulence gene repertoire

Penna, Bruno; Silva, Marcella B.; Soares, André E. R.; Vasconcelos, Ana Tereza Ribeiro de; Ramundo, Mariana Severo; Ferreira, Fabienne Antunes; Silva-Carvalho, Maria Cícera; Sousa, Viviane Santos de; Rabello, Renata F.; Bandeira, Paula Terra; Souza, Viviane Silva de; Planet, Paul J.; Vieira‐da‐Motta, Olney; Botelho, Ana; Figueiredo, Agnes Marie Sá

doi:10.1038/s41598-021-83993-5

Cited by 19 publications

(15 citation statements)

References 73 publications

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“…In this study, the overall oral carriage of MRSA from dogs was 15 % (15/100), which was higher than the reports by S a s a k i et al [32], A b b o t t et al [1], K o t t l e r et al [19], P e n n a et al [30] L o e f f l e r et al [22] and C h a h et al [4] who, respectively, recorded 1.7 %, 1.1 %, 3.3 %, 3.4 %, 9.0 % and 12.8 % of MRSA in the dogs in the Ireland, UK, the US, and Nigeria, respectively.…”

Section: Discussioncontrasting

confidence: 66%

Evidence of Methicillin Resistant Staphylococcus Aureus (MRSA) in Pet and Stray Dogs Within Sokoto Metropolis, Nigeria

Yakubu

Gaddafi²,

Musawa

et al. 2022

Folia Veterinaria

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Methicillin Resistant Staphylococcus aureus (MRSA) is an important zoonotic pathogen capable of causing life threatening disease conditions in humans. A cross-sectional study was conducted to investigate the presence of MRSA in both pet and stray dogs within the Sokoto metropolis. A total of 100 oral swabs comprising 50 each from pet and stray dogs were collected and analyzed using routine bacteriological cultures and molecular identifications. Out of the 100 samples examined, 15 % (15/100) were positive for MRSA with varying detection rates of 9/50 (18 %) and 6/50 (12 %) for the pet and stray dogs respectively. The statistical analysis showed no significant association between the occurrence of MRSA and the dogs (P = 0.401). The study revealed the presence of MRSA in dogs within the Sokoto metropolis, which presents health risks to pet dog owners, veterinarians, dog catchers and other individuals who may come into close contact with these dogs.

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Section: Discussioncontrasting

confidence: 66%

Evidence of Methicillin Resistant Staphylococcus Aureus (MRSA) in Pet and Stray Dogs Within Sokoto Metropolis, Nigeria

Yakubu

Gaddafi²,

Musawa

et al. 2022

Folia Veterinaria

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“…The inter-habitat traversing and the frequent contact between wild animals, livestock and the indirect contact with humans can increase bacterial transmission and often promote the risks of colonisation and infections in humans and animals [ 3 , 4 , 5 ]. Antimicrobial-resistant bacteria spread by anthropogenic sources, such as industrial and domestic wastewater effluents, agricultural runoff and garbage, have been suspected to be the primary link to wild animals [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Wild Animals Are Reservoirs and Sentinels of Staphylococcus aureus and MRSA Clones: A Problem with “One Health” Concern

et al. 2021

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Background: The availability of comprehensive data on the ecology and molecular epidemiology of Staphylococcus aureus/MRSA in wild animals is necessary to understand their relevance in the “One Health” domain. Objective: In this study, we determined the pooled prevalence of nasal, tracheal and/or oral (NTO) Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA) carriage in wild animals, with a special focus on mecA and mecC genes as well as the frequency of MRSA and methicillin susceptible S. aureus (MSSA) of the lineages CC398 and CC130 in wild animals. Methodology: This systematic review was executed on cross-sectional studies that reported S. aureus and MRSA in the NTO cavities of wild animals distributed in four groups: non-human primates (NHP), wild mammals (WM, excluding rodents and NHP), wild birds (WB) and wild rodents (WR). Appropriate and eligible articles published (in English) between 1 January 2011 to 30 August 2021 were searched for from PubMed, Scopus, Google Scholar, SciElo and Web of Science. Results: Of the 33 eligible and analysed studies, the pooled prevalence of NTO S. aureus and MRSA carriage was 18.5% (range: 0–100%) and 2.1% (range: 0.0–63.9%), respectively. The pooled prevalence of S. aureus/MRSA in WM, NHP, WB and WR groups was 15.8/1.6, 32.9/2.0, 10.3/3.4 and 24.2/3.4%, respectively. The prevalence of mecC-MRSA among WM/NHP/WB/WR was 1.64/0.0/2.1/0.59%, respectively, representing 89.9/0.0/59.1/25.0% of total MRSA detected in these groups of animals.The MRSA-CC398 and MRSA-CC130 lineages were most prevalent in wild birds (0.64 and 2.07%, respectively); none of these lineages were reported in NHP studies. The MRSA-CC398 (mainly of spa-type t011, 53%), MRSA-CC130 (mainly of spa types t843 and t1535, 73%), MSSA-CC398 (spa-types t571, t1451, t6606 and t034) and MSSA-CC130 (spa types t843, t1535, t3625 and t3256) lineages were mostly reported. Conclusion: Although the global prevalence of MRSA is low in wild animals, mecC-mediated resistance was particularly prevalent among MRSA isolates, especially among WM and WB. Considering the genetic diversity of MRSA in wild animals, they need to be monitored for effective control of the spread of antimicrobial resistance.

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“…Antimicrobial resistance is a relevant health problem worldwide that needs the development of new strategies. The human–animal–environment interface is currently being considered under the OneHealth approach to better understand the ecology and dissemination of AMR and to control the silent pandemy, as is considered by some authors ( Swift et al, 2019 ; Penna et al, 2021 ). In this context, the use of probiotics or new antimicrobial compounds, such as bacteriocins, is an interesting alternative to the use of conventional antibiotics.…”

Section: Discussionmentioning

confidence: 99%

Bacteriocin-Like Inhibitory Substances in Staphylococci of Different Origins and Species With Activity Against Relevant Pathogens

et al. 2022

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Bacteriocins are antimicrobial peptides with relevance in the modulation of human and animal microbiota that have gained interest in biomedical and biotechnological applications. In this study, the production of bacteriocin-like inhibitory substances (BLIS) was tested among a collection of 890 staphylococci of different origins (humans, animals, food, and the environment) and species, both coagulase-positive (CoPS, 238 isolates of 3 species) and coagulase-negative staphylococci (CoNS, 652 isolates of 26 species). Of the 890 staphylococci, 60 (6.7%) showed antimicrobial activity by the spot-on-lawn method against at least one of the 25 indicator bacteria tested. BLIS-producer (BLIS+) isolates were detected in 8.8% of CoPS and 6.0% of CoNS. The staphylococcal species with the highest percentages of BLIS+ isolates were S. chromogenes (38.5%), S. pseudintermedius (26.7%), and S. warneri (23.1%). The production of BLIS was more frequently detected among isolates of pets, wild animals, and food. Moreover, 13 BLIS+ isolates showed wide antimicrobial activiy spectrum, and 7 of these isolates (of species S. aureus, S. pseudintermedius, S. sciuri, and S. hominis) demonstrated antimicrobial activity against more than 70% of the indicator bacteria tested. The genetic characterization (by PCR and sequencing) of the 60 BLIS+ isolates revealed the detection of (a) 11 CoNS and CoPS isolates carrying putative lantibiotic-like genes; (b) 3 S. pseudintermedius isolates harboring the genes of BacSp222 bacteriocin; and (c) 2 S. chromogenes isolates that presented the gene of a putative cyclic bacteriocin (uberolysin-like), being the first report in this CoNS species. Antimicrobial susceptibility testing was performed in BLIS+ isolates and one-third of the CoNS isolates showed susceptibility to all antibiotics tested, which also lacked the virulence genes studied. These BLIS+ CoNS are good candidates for further characterization studies.

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Comparative genomics of MRSA strains from human and canine origins reveals similar virulence gene repertoire

Cited by 19 publications

References 73 publications

Evidence of Methicillin Resistant Staphylococcus Aureus (MRSA) in Pet and Stray Dogs Within Sokoto Metropolis, Nigeria

Evidence of Methicillin Resistant Staphylococcus Aureus (MRSA) in Pet and Stray Dogs Within Sokoto Metropolis, Nigeria

Wild Animals Are Reservoirs and Sentinels of Staphylococcus aureus and MRSA Clones: A Problem with “One Health” Concern

Bacteriocin-Like Inhibitory Substances in Staphylococci of Different Origins and Species With Activity Against Relevant Pathogens

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