Comparative genomics of Photobacterium species from terrestrial and marine habitats

Abstract

Highlights Photobacteria exploit horizontal gene transfer for niche adaptation. P. phosphoreum divides into two subspecies with no correlation to isolation origin or metabolism. P. phosphoreum has wider tools for stress response and antimicrobial activity. P. iliopiscarium divides in two clades (marine/terrestrial) with distinct environmental adaptation. P. carnosum forms by one single clade with wide sugar utilization.

Impact of Modified Atmospheres on Growth and Metabolism of Meat-Spoilage Relevant Photobacterium spp. as Predicted by Comparative Proteomics

“…Unlike P. phosphoreum , showing no differential detection, results suggest that P. carnosum has a higher sensitivity and, therefore, has an earlier response to stress. This was also supported by previously predicted higher sensitivity to oxidative stress (Fuertes-Perez et al, 2021 ) and the demonstrated sensitivity to other types of stress such as high pressure, temperature, and salt concentration (Hilgarth et al, 2018b ; Hauschild et al, 2020 ).…”

“…The synthesis of an additional proton-translocating NADH dehydrogenase complex by P. phosphoreum might influence the efficiency of the respiratory chain and explain, to some extent, the aerobically faster growth of the species in comparison to P. carnosum . As predicted before by Fuertes-Perez et al ( 2021 ), they use both the non-electrogenic and sodium-translocating version of Complex I, which is in agreement with the sodium requirement of these bacteria (Hilgarth et al, 2018a , b ). Additionally, P. phosphoreum also synthesizes the proton-translocating version.…”

“…According to a comparative genomics study on photobacteria reported by Fuertes-Perez et al ( 2021 ), all strains that were analyzed encode a complete respiratory chain in their genomes. Figure 2 contains an entire summary of genes present in the genomes of each strain and a summary of protein detection.…”

“…The accumulation of O 2 consuming acetolactate synthase enzyme in P. carnosum that strains under oxic conditions is also interpreted as an adaptive mechanism of the bacteria to the environmental gas atmosphere. In addition, the biosynthesis of valine, leucine, and isoleucine is upregulated under oxic conditions for P. carnosum ( Figure 3 ), among the most common amino acids in the proteome of the species (Fuertes-Perez et al, 2021 ).…”

“…Strains of both species were selected as representative isolates from raw meat. Two strains per species were chosen to cover their previously reported high intra-species variability (Fuertes-Perez et al, 2019 , 2021 ). P. carnosum TMW 2.2021 T (DSM 105454 T ) is the described type of strain of the species (Hilgarth et al, 2018b ) that was isolated from MAP raw chicken meat.…”