Comparative Hepatology: A journal for all hepatologists with immediate Open Access to quality peer-reviewed research

Rocha, Eduardo Arrais; Hinton, David E.; Wisse, Eddie

doi:10.1186/1476-5926-3-1

Cited by 6 publications

(4 citation statements)

References 4 publications

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“…The Aryl hydrocarbon (AhR) receptor is activated upon exposure to several PBTs (including PAHs, PCBs and PBDEs) as a defense mechanism to aid metabolization 39 . After activation, AhR translocates to the nucleus to dimerize with ARNT, leading to changes in xenobiotic response-related gene transcription 40 .…”

Section: Resultsmentioning

confidence: 99%

Ingested plastic transfers hazardous chemicals to fish and induces hepatic stress

Rochman

Hoh

Kurobe

et al. 2013

Sci Rep

1,487

699

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Plastic debris litters aquatic habitats globally, the majority of which is microscopic (< 1 mm), and is ingested by a large range of species. Risks associated with such small fragments come from the material itself and from chemical pollutants that sorb to it from surrounding water. Hazards associated with the complex mixture of plastic and accumulated pollutants are largely unknown. Here, we show that fish, exposed to a mixture of polyethylene with chemical pollutants sorbed from the marine environment, bioaccumulate these chemical pollutants and suffer liver toxicity and pathology. Fish fed virgin polyethylene fragments also show signs of stress, although less severe than fish fed marine polyethylene fragments. We provide baseline information regarding the bioaccumulation of chemicals and associated health effects from plastic ingestion in fish and demonstrate that future assessments should consider the complex mixture of the plastic material and their associated chemical pollutants.

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Section: Resultsmentioning

confidence: 99%

Ingested plastic transfers hazardous chemicals to fish and induces hepatic stress

Rochman

Hoh

Kurobe

et al. 2013

Sci Rep

1,487

699

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“…However, an involvement of Kupffer cells in the pathophysiology of APAP hepatotoxicity has been highly controversial. Although it was originally reported that mice pretreated with gadolinium chloride (GdCl 3 ), an intervention that inactivates Kupffer cells, had reduced peroxynitrite formation and were protected against APAP-induced liver injury [88] , neither of these results could be reproduced by subsequent studies [89] , [90] , [91] . In support of these later reports, mice functionally deficient in NADPH oxidase activity (gp91phox -/- mice) did not show a decreased oxidative stress or reduced liver injury [92] , [93] .…”

Section: Sources and Relevance Of Oxidant Stress In Acetaminophen Hepmentioning

confidence: 99%

Oxidative stress during acetaminophen hepatotoxicity: Sources, pathophysiological role and therapeutic potential

2016

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Acetaminophen (APAP) hepatotoxicity is characterized by an extensive oxidative stress. However, its source, pathophysiological role and possible therapeutic potential if targeted, have been controversially described. Earlier studies argued for cytochrome P450-generated reactive oxygen species (ROS) during APAP metabolism, which resulted in massive lipid peroxidation and subsequent liver injury. However, subsequent studies convincingly challenged this assumption and the current paradigm suggests that mitochondria are the main source of ROS, which impair mitochondrial function and are responsible for cell signaling resulting in cell death. Although immune cells can be a source of ROS in other models, no reliable evidence exists to support a role for immune cell-derived ROS in APAP hepatotoxicity. Recent studies suggest that mitochondrial targeted antioxidants can be viable therapeutic agents against hepatotoxicity induced by APAP overdose, and re-purposing existing drugs to target oxidative stress and other concurrent signaling events can be a promising strategy to increase its potential application in patients with APAP overdose.

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“…LSEC line the sinusoidal wall and facilitate the exchange of fluids, solutes and particles between the liver sinusoidal blood and the space of Disse by dynamic filters, called fenestrae [ 12 ]. In addition, LSEC exhibit high endocytic capability and eliminate numerous substances from the blood by receptor-mediated endocytosis [ 13 ]. HSC reside in the subendothelial space [ 14 ] and were first described by Carl von Kupffer in 1876 as star-shaped cells that react to gold chloride [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

All-In-One: Advanced preparation of Human Parenchymal and Non-Parenchymal Liver Cells

et al. 2015

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Background & AimsLiver cells are key players in innate immunity. Thus, studying primary isolated liver cells is necessary for determining their role in liver physiology and pathophysiology. In particular, the quantity and quality of isolated cells are crucial to their function. Our aim was to isolate a large quantity of high-quality human parenchymal and non-parenchymal cells from a single liver specimen.MethodsHepatocytes, Kupffer cells, liver sinusoidal endothelial cells, and stellate cells were isolated from liver tissues by collagenase perfusion in combination with low-speed centrifugation, density gradient centrifugation, and magnetic-activated cell sorting. The purity and functionality of cultured cell populations were controlled by determining their morphology, discriminative cell marker expression, and functional activity.ResultsCell preparation yielded the following cell counts per gram of liver tissue: 2.0±0.4×107 hepatocytes, 1.8±0.5×106 Kupffer cells, 4.3±1.9×105 liver sinusoidal endothelial cells, and 3.2±0.5×105 stellate cells. Hepatocytes were identified by albumin (95.5±1.7%) and exhibited time-dependent activity of cytochrome P450 enzymes. Kupffer cells expressed CD68 (94.5±1.2%) and exhibited phagocytic activity, as determined with 1μm latex beads. Endothelial cells were CD146+ (97.8±1.1%) and exhibited efficient uptake of acetylated low-density lipoprotein. Hepatic stellate cells were identified by the expression of α-smooth muscle actin (97.1±1.5%). These cells further exhibited retinol (vitamin A)-mediated autofluorescence.ConclusionsOur isolation procedure for primary parenchymal and non-parenchymal liver cells resulted in cell populations of high purity and quality, with retained physiological functionality in vitro. Thus, this system may provide a valuable tool for determining liver function and disease.

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Comparative Hepatology: A journal for all hepatologists with immediate Open Access to quality peer-reviewed research

Cited by 6 publications

References 4 publications

Ingested plastic transfers hazardous chemicals to fish and induces hepatic stress

Ingested plastic transfers hazardous chemicals to fish and induces hepatic stress

Oxidative stress during acetaminophen hepatotoxicity: Sources, pathophysiological role and therapeutic potential

All-In-One: Advanced preparation of Human Parenchymal and Non-Parenchymal Liver Cells

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